Neuroprotective effects of neuropeptide Y-Y2 and Y5 receptor agonists in vitro and in vivo

Śmiałowska, Maria; Domin, Helena; Zieba, Barbara A.; Koźniewska, Ewa; Michalik, R; Piotrowski, Piotr; Kajta, Małgorzata

doi:10.1016/j.npep.2009.02.002

Cited by 59 publications

(47 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5,95,96 In rat and mouse organotypic hippocampal cultures, the involvement of NPY Y 1 , Y 2 , and/or Y 5 receptors have been shown in the protection against cell death induced by glutamate. 95,97,98 In contrast, we have shown that NPY has a protective role against 3,4-methylenedioxy-N-methylamphetamine (MDMA)-induced toxicity in rat retinal cells. 31 86 Western blot analysis of rat hippocampus, rat whole brain, and dentate gyrus neurospherederived cultures reveals a 50 kDa single band.…”

Section: Discussionmentioning

confidence: 97%

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Santos-Carvalho

Aveleira²,

Elvas

et al. 2013

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE. Neuropeptide Y (NPY) is one of the most abundant peptides in the central nervous system (CNS), including the retina. This peptide activates various different G-coupled receptors (NPY Y 1 , Y 2 , Y 4 , and Y 5 ) that are also present in the retina. However, the localization of NPY receptors in the several types of retinal cells is not completely known. In this study, we have looked at the distribution of NPY Y 1 and Y 2 receptors in rat retinal cells to reveal new perspectives on the role of NPY receptors in retina physiology. METHODS.Rat retinal neural cell cultures were prepared from newborn Wistar rats (P3-P5) and pure rat Müller cell culture was obtained after treatment of these cells with ascorbic acid. 1 This 36-amino acid peptide was first isolated from the pig brain in 1982 by Tatemoto.2 NPY is one of the most abundant peptides in the central nervous system (CNS), including the retina, and is involved in numerous physiologic functions, such as feeding, memory processing, and cognition.3,4 NPY actions are mediated by G protein-coupled receptors, which have been named NPY Y 1 , Y 2 , Y 4 , Y 5 , and y 6 . 3,5 NPY is expressed in the retina of mammals and nonmammals. [6][7][8][9][10][11][12] In rodent retinas, NPY-immunoreactivity (NPY-ir) is present in the inner retina, it is localized in cell bodies at both inner nuclear layer (INL) and ganglion cell layer (GCL), and is also present in processes located in the inner plexiform layer (IPL).11,13 NPY shows one of the highest degrees of phylogenetic preservation, compared with other neuropeptides, and does not show marked differences between species.14,16 From a consideration of the phylogenetic preservation, it is expected that the NPY and NPY receptor distribution in the retina does not vary significantly between species. NPY-ir is present in amacrine cells in the majority of species studied (fish, frogs, lizards, rodents, baboons, pigs, cats, chickens, and pigeons). Cats, dogs, dolphins, and humans also show NPY in their ganglion cells, 9,17-20 while some turtles, lizards, and frogs present NPY-ir in bipolar cells. 8,21,22 Few studies have analyzed the distribution of NPY receptors either in the retina or in specific retinal cell types. The presence of both Y 1 and Y 2 receptors in the mouse retina has been confirmed by mRNA analysis 13,23 ; however, it is unclear which cell types express these receptors. Moreover, in primary cultures of Müller cells isolated from the retina of guinea pig, a functional assay of cell proliferation suggested the presence of NPY Y 1 receptor in these glial cells. 24 In human retinal pigment epithelium (RPE), mRNA encoding for NPY Y 1 , Y 2 , and Y 5 receptors has been detected, while in bovine RPE only mRNAs encoding for NPY Y 1 and Y 2 receptors have been found. 25 Although the presence of NPY receptors in the rat retina has been described, in particular in NPY Y 1 , Y 2 , Y 4 , and Y 5 mRNA expression, 26,27 little is known about their localization. The NPY Y 1 receptor-ir is localized in horizontal and amacrine ce...

show abstract

Section: Discussionmentioning

confidence: 97%

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Santos-Carvalho

Aveleira²,

Elvas

et al. 2013

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…Interestingly, some studies have discovered that NPY is able to inhibit neuronal excitability in in vivo and in vitro models [48] , through a mechanism involving the inhibition of the voltage-dependent Ca 2+ influx in presynaptic nerve terminals [49][50][51][52] , an effect probably mediated by activation of the NPY Y(2) receptor [53,54,11] . It is generally assumed that glutamatergic overactivation may lead to excitotoxic cell death and that inhibition of the toxic glutamatergic hyperactivity may produce neuroprotection.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effect of Neuropeptide Y against Beta-Amyloid 25–35 Toxicity in SH-SY5Y Neuroblastoma Cells Is Associated with Increased Neurotrophin Production

Croce

Dinallo²,

Ricci

et al. 2011

Neurodegener Dis

View full text Add to dashboard Cite

Background: In the central nervous system, several neuropeptides are believed to be involved in the pathophysiology of Alzheimer’s disease (AD). Among them, neuropeptide Y (NPY) is a small peptide widely distributed throughout the brain, where it serves as a neurotransmitter and/or a modulator of several neuroendocrine functions. More recently, NPY has generated interest because of its role in neuroprotection against excitotoxicity and modulation of neurogenesis. Interestingly, these effects are also influenced by neurotrophins, critical molecules for the function and survival of neurons that degenerate in AD. Objective: Our purpose was to investigate whether NPY might be a neuroprotective agent in AD and whether neurotrophins are involved in NPY-induced neuroprotection. Methods: To test this hypothesis, we exposed the SH-SY5Y neuroblastoma cell line to toxic concentrations of β-amyloid (Aβ) peptide fragment 25–35 (Aβ_25–35) and measured cell survival and neurotrophin expression before and after a preincubation with NPY in the growth medium. Results: Our results demonstrated that preincubation with NPY prevented cell loss due to the toxic effect of Aβ_25–35. Moreover, while intracellular production of nerve growth factor and brain-derived neurotrophic factor were reduced by Aβ, NPY restored or even increased neurotrophin protein and mRNA in SH-SY5Y cells. Conclusion: In conclusion, this study demonstrates that NPY increases the survival of SH-SY5Y neuroblastoma cells and counteracts the toxic effect of Aβ. In addition, NPY restores the neurotrophin levels in these cells. Although preliminary, these observations might be useful to understand the pathology of Alzheimer’s and/or develop new therapeutic strategies.

show abstract

“…Since specific agonists and antagonists of those receptors have been synthesized (Aguirre et al, 1990;Cabrele et al, 2000;Doods et al, 1999;Dumont et al, 2000;Fuhlendorff et al, 1990;Rudolf et al, 1994;Wieland et al, 1998) the studies on the role of particular receptors in neuroprotection became possible. Most of evidences demonstrated neuroprotective effects after Y2 and Y5 receptor agonists (Silva et al, 2003b(Silva et al, , 2005aŚmialowska et al, 2009;Wu & Li, 2005). Neuroprotective role of Y2 and Y5 receptor stimulation was also suggested by electrophysiological and behavioral studies in which the activation of these receptors suppressed epileptiform bursting or seizures (Bijak, 1999;Klapstein & Colmers, 1997;Marsh et al, 1999;Woldbye et al, 1997;Vezzani et al, 1999).…”

Section: The Role Of Particular Y Receptors In Neuroprotectionmentioning

confidence: 98%

Neuroprotective Effects of Neuropeptide Y and Y2 and Y5 Receptor Agonists In Vitro and In Vivo

Śmiałowska¹,

Domi²

2012

Neurodegeneration

View full text Add to dashboard Cite

Neuroprotective effects of neuropeptide Y-Y2 and Y5 receptor agonists in vitro and in vivo

Cited by 59 publications

References 72 publications

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Neuroprotective Effect of Neuropeptide Y against Beta-Amyloid 25–35 Toxicity in SH-SY5Y Neuroblastoma Cells Is Associated with Increased Neurotrophin Production

Neuroprotective Effects of Neuropeptide Y and Y2 and Y5 Receptor Agonists In Vitro and In Vivo

Contact Info

Product

Resources

About

Neuroprotective effects of neuropeptide Y-Y2 and Y5 receptor agonists in vitro and in vivo

Cited by 59 publications

References 72 publications

Neuropeptide Y Receptors Y1and Y2are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Neuropeptide Y Receptors Y1and Y2are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Neuroprotective Effect of Neuropeptide Y against Beta-Amyloid 25–35 Toxicity in SH-SY5Y Neuroblastoma Cells Is Associated with Increased Neurotrophin Production

Neuroprotective Effects of Neuropeptide Y and Y2 and Y5 Receptor Agonists In Vitro and In Vivo

Contact Info

Product

Resources

About

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture