Neuroprotective effects of Camellia nitidissima Chi leaf extract in hydrogen peroxide‐treated human neuroblastoma cells and its molecule mechanisms

Abstract

Camellia nitidissima Chi (CNC) is a famous medicinal and edible plant with the name of “Tea for Longevity” in Guangxi province of China. In present study, we determined the protective effect of extract from CNC leaves on H2O2‐induced cell injury and its underlying mechanisms in human neuroblastoma (SH‐SY5Y) cells. The ethyl acetate fraction of CNC leaves (CLE, 50–200 μg/ml) treatment significantly increased the cell viability of H2O2‐treated SH‐SY5Y cells and reduced the leakage of LDH in a reversed “U”‐shape manner. It was confirmed by Hoechst 33,342 staining that CLE attenuated H2O2‐induced apoptosis in SH‐SY5Y cells. The CLE (100 and 150 μg/ml) treatment significantly relieved H2O2‐induced oxidative stress by decreasing intracellular ROS level, and increasing the activities of superoxide dismutase (SOD) and catalase (CAT). Western blot analysis demonstrated that the CLE treatment reserved H2O2‐induced decrease of pCREB (Ser133) expression, and its downstream protein BDNF. In addition, 37 phenolic compounds in CLE were identified by UPLC‐TOF MS/MS, and the main active phytochemicals seemed to be catechins, quercetin, kaempferol, and their derivatives. In conclusion, the data analysis showed that the neuroprotective effect of CNC leaves might be achieved via synergistically boosting endogenous antioxidant defenses and neurotrophic signaling pathway. These results suggest that CNC leaves are valuable resources for functional foods and beverages.

Quercetin: A Bioactive Compound Imparting Cardiovascular and Neuroprotective Benefits: Scope for Exploring Fresh Produce, Their Wastes, and By-Products

“…This treatment reduces the effects of diabetes on nNOS-IR neurons and VIP-IR varicosities in the myenteric plexus of experimental diabetic rats, as reported by Martins-Perles et al [87]. The presence of quercetin in Camellia nitidissima Chi, 'Tea for Longevity' a well-known plant consumed as both medicine and food in China, was studied for its neuroprotective effect by An et al [88]. Based on two used terms, 'synergistically boosting endogenous antioxidant defences' and 'neurotrophic signalling pathway' by An et al [88], this plant has been cited as an important source for phytonutrients, including quercetin for edible foods and beverages.…”

“…The presence of quercetin in Camellia nitidissima Chi, 'Tea for Longevity' a well-known plant consumed as both medicine and food in China, was studied for its neuroprotective effect by An et al [88]. Based on two used terms, 'synergistically boosting endogenous antioxidant defences' and 'neurotrophic signalling pathway' by An et al [88], this plant has been cited as an important source for phytonutrients, including quercetin for edible foods and beverages. Soxhlet extraction to yield quercetin along with other phytoconstituents from Pinus roxburghii Sarg.…”

Toxicological Evaluation of Camellia euphlebia Leaves Aqueous Extract Using Acute and Subacute Toxicity Studies in Mice and Genotoxicity Studies

“…Camellia euphlebia Merr. ex Sealy (genus Camellia ), praised as “flora panda,” is not only a rare ornamental species limitedly distributed in Southwest China and Northern Vietnam but also a novel food source authorized by the Chinese Ministry of Health [ 4 , 5 ]. The great potential of C. euphlebia , either in respect of traditional folk medicine (urinary tract infection, dysentery, faucitis, and irregular menstruation) or modern pharmacological findings (antitumor, hypolipidemic, antidepressant, and antioxidant), has attracted considerable attention worldwide as a valuable resource for natural drugs [ 6 ].…”

Application of FT-IR spectroscopy and chemometric technique for the identification of three different parts of Camellia nitidissima and discrimination of its authenticated product

“…In addition to offering functional benefits to the community via non-pharmacological and lifestyle adaptations, it has been discovered to exert a wide range of pharmacological effects. It can be an useful agent with anticancer, antioxidant, hypolipidemic, antidiabetic, antiallergic, antibacterial, and anxiolytic properties ( He et al, 2017 ; An et al, 2020 ). Previous research study has shown that C.nitidissima has neuroprotective benefits through a synergistic interaction between antioxidant and neurotrophic signaling pathways ( An et al, 2020 ).…”