Neuroprotective effects of hydrogen saline in neonatal hypoxia–ischemia rat model

Jian-Hua, Cai; Kang, Zhiwei; Liu, Kan; Liu, Wenwu; Li, Runping; Zhang, Junyi; Luo, Xu; Sun, Xuejun

doi:10.1016/j.brainres.2008.11.048

Cited by 206 publications

(175 citation statements)

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“…Therefore, the results suggest that the protective roles of H 2 treatment may be associated with reduced SGN apoptosis. In addition, H 2 treatment can reduce the levels of oxidative products and inflammatory cytokines, as well as increase antioxidant enzymatic activities in many diseases [4,6,[8][9][10][11][12][13][14] . Further studies are needed to determine whether these mechanisms contribute to the protective effects of H 2 treatment against AN.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, many studies, including ours, have found that H 2 has therapeutic roles in many diseases via its ability to reduce oxidative stress, inflammation and apoptosis; these diseases include cerebral damage, spinal cord injury, sepsis, multiple organ dysfunction, chronic allograft nephropathy, tumors and type 2 diabetes [4][5][6][7][8][9][10][11][12][13][14] . Furthermore, H 2 can effectively protect against the morphological and functional cochlear hair cell damage induced by ROS [15] .…”

Section: Introductionmentioning

confidence: 97%

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Inhalation of hydrogen gas attenuates ouabain-induced auditory neuropathy in gerbils

Xie

et al. 2012

Acta Pharmacol Sin

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Aim: Auditory neuropathy (AN) is a hearing disorder characterized by abnormal auditory nerve function with preservation of normal cochlear hair cells. This study was designed to investigate whether treatment with molecular hydrogen (H 2 ), which can remedy damage in various organs via reducing oxidative stress, inflammation and apoptosis, is beneficial to ouabain-induced AN in gerbils. Methods: AN model was made by local application of ouabain (1 mmol/L, 20 mL) to the round window membrane in male Mongolian gerbils. H 2 treatment was given twice by exposing the animals to H 2 (1%, 2%, and 4%) for 60 min at 1 h and 6 h after ouabain application. Before and 7 d after ouabain application, the hearing status of the animals was evaluated using the auditory brainstem response (ABR) approach, the hear cell function was evaluated with distortion product otoacoustic emissions (DPOAE). Seven days after ouabain application, the changes in the cochleae, especially the spiral ganglion neurons (SGNs), were morphologically studied. TUNEL staining and immunofluorescent staining for activated caspase-3 were used to assess the apoptosis of SGNs. Results: Treatment with H 2 (2% and 4%) markedly attenuated the click and tone burst-evoked ABR threshold shift at 4, 8, and 16 kHz in ouabain-exposed animals. Neither local ouabain application, nor H 2 treatment changed the amplitude of DPOAE at 4, 8, and 16 kHz. Morphological study showed that treatment with H 2 (2%) significantly alleviated SGN damage and attenuated the loss of SGN density for each turn of cochlea in ouabain-exposed animals. Furthermore, ouabain caused significantly higher numbers of apoptotic SGNs in the cochlea, which was significantly attenuated by the H 2 treatment. However, ouabain did not change the morphology of cochlear hair cells. Conclusion:The results demonstrate that H 2 treatment is beneficial to ouabain-induced AN via reducing apoptosis. Thus, H 2 might be a potential agent for treating hearing impairment in AN patients.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Inhalation of hydrogen gas attenuates ouabain-induced auditory neuropathy in gerbils

Xie

et al. 2012

Acta Pharmacol Sin

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“…Preparation of HW HW was produced as that in previous studies. 21) In short, purified hydrogen gas was dissolved into water under the pressure of 0.6 MPa for 2 h and stored in an aluminum bag without dead volume at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Hydrogen Rich Water Attenuates Renal Injury and Fibrosis by Regulation Transforming Growth Factor-β Induced Sirt1

Zhou¹,

Pan

Zhang

et al. 2017

Biological & Pharmaceutical Bulletin

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“…There are several recent studies reported that molecular hydrogen reduced oxidative stress and its associated disorders. Molecular hydrogen in the form of gas or H 2 -saturated saline reduced the cerebral infarction (Ohsawa et al,2007) and decreased apoptosis in neonatal hypoxic brain injury in rats (Cai et al,2008;Cai et al,2009). Molecular hydrogen dissolved in drinking water similarly attenuated sclerotic lesions (Ohsawa et al,2008) and prevented cisplatin-induced nephrotoxicity in mice (Nakashima et al,2009).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer's disease by reduction of oxidative stress

et al. 2010

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Cao, Xue-jun Sun, Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer's disease by reduction of oxidative stress, Brain Research (2010Research ( ), doi: 10.1016Research ( /j.brainres.2010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A C C E P T E D M A N U S C R I P T A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 2 AbstractThis study is to examine if hydrogen-rich saline reduced amyloid β (Aβ) induced neural inflammation, and learning and memory deficits in a rat model. S-D male rats (n = 84, 280-330g) were divided into three groups, sham operated, Aβ1-42 injected and Aβ1-42 plus hydrogen-rich saline treated animals. Hydrogen-rich saline (5 ml/kg, i.p., daily) was injected for 14 days after intracerebroventricular injection of Aβ1-42. The levels of MDA, IL-6 and TNF-α were assessed by biochemical and ELISA analysis. Morris Water Maze and open field task were used to assess the memory dysfunction and motor dysfunction, respectively. LTP were used to detect the electrophysiology changes, HNE and GFAP immunohistochemistry were used to assess the oxidative stress and glial cell activation. After Aβ1-42 injection, the levels of MDA, IL-6, and TNF-α were increased in brain tissues and hydrogen-rich saline treatment suppressed MDA, IL-6, and TNF-α concentration. Hydrogen-rich saline treatment improved Morris Water Maze and enhanced LTP in hippocampus blocked by Aβ1-42.Furthermore, hydrogen-rich saline treatment also decreased the immunoreactivitiy of HNE and GFAP in hippocampus induced by Aβ1-42. In conclusion, hydrogen-rich saline prevented Aβ-induced neuroinflammation and oxidative stress, which may contribute to the improvement of memory dysfunction in this rat model.

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Neuroprotective effects of hydrogen saline in neonatal hypoxia–ischemia rat model

Cited by 206 publications

References 23 publications

Inhalation of hydrogen gas attenuates ouabain-induced auditory neuropathy in gerbils

Inhalation of hydrogen gas attenuates ouabain-induced auditory neuropathy in gerbils

Hydrogen Rich Water Attenuates Renal Injury and Fibrosis by Regulation Transforming Growth Factor-β Induced Sirt1

Hydrogen-rich saline improves memory function in a rat model of amyloid-beta-induced Alzheimer's disease by reduction of oxidative stress

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