Neuroprotective effects of Fomes officinalis Ames polysaccharides on Aβ25–35-induced cytotoxicity in PC12 cells through suppression of mitochondria-mediated apoptotic pathway

Habaike, Ayijiang; Mirensha, Yakufu; Yao, Cong; Gahafu, Yimin; Li, Zhen; Abulizi, Palida

doi:10.1007/s10616-020-00400-z

Cited by 13 publications

(15 citation statements)

References 24 publications

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“…As an anti-apoptotic member of the Bcl-2 family, Bcl-2 can bind Bax to form Bax/Bcl-2 heterodimers, thereby, attenuating the pro-apoptotic effect of Bax. Habaike et al found polysaccharides extracted from Laricifomes officinalis Ames could attenuating cell apoptosis, increasing the ratio of Bcl-2/Bax and inhibiting cytochrome C release from mitochondria to cytosol in PC12 cells [23]. An acid-soluble polysaccharide (GFAP) prepared from Grifola frondosa could upregulate the expressions of Bax in HCC cells and induced the cell apoptosis [24].…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Fucoidan against MPP+-Induced SH-SY5Y Cells Apoptosis by Affecting the PI3K/Akt Pathway

et al. 2020

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The main pathologic changes of the Parkinson’s disease (PD) is dopaminergic (DA) neurons lost. Apoptosis was one of the important reasons involved in the DA lost. Our previous study found a fucoidan fraction sulfated heterosaccharide (UF) had neuroprotective activity. The aim of this study was to clarify the mechanism of UF on DA neurons using human dopaminergic neuroblastoma (SH-SY5Y) cells a typical as a PD cellular model. Results showed that UF prevented MPP+-induced SH-SY5Y cells apoptosis and cell death. Additionally, UF pretreated cells increased phosphorylation of Akt, PI3K and NGF, which means UF-treated active PI3K–Akt pathway. Moreover, UF treated cells decreased the expression of apoptosis-associated protein, such as the ratio of Bax/Bcl-2, GSK3β, caspase-3 and p53 nuclear induced by MPP+. This effect was partially blocked by PI3K inhibitor LY294002. Our data suggested that protective effect of UF against MPP+-induced SH-SY5Y cells death by affecting the PI3K–Akt pathway. These findings contribute to a better understanding of the critical roles of UF in treating PD and may elucidate the molecular mechanisms of UF effects in PD.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Fucoidan against MPP+-Induced SH-SY5Y Cells Apoptosis by Affecting the PI3K/Akt Pathway

et al. 2020

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“…Polysaccharide fractions significantly inhibited the over accumulation of ROS induced by Aβ25-35 and the release of LDH and MDA, dependent on their intake [149]. FOAPs may also prevent cell apoptosis [149]. Translocation of cytochrome C from mitochondria to the cytosol was decreased, and the Bcl-2/Bax ratio was raised in neuronal cells in response to FOAPs.…”

Section: Antioxidant Activity Of Polysaccharidesmentioning

confidence: 99%

“…Habaike et al [149] aimed to identify the protective roles of various components in Fomes officinalis Ames polysaccharides (FOAPs) in neuronal cells. Various concentrations of FOAPs were applied to neuronal cells two hours prior to direct exposure to a β-amyloid protein fragment [25][26][27][28][29][30][31][32][33][34][35].…”

Section: Antioxidant Activity Of Polysaccharidesmentioning

confidence: 99%

“…Various concentrations of FOAPs were applied to neuronal cells two hours prior to direct exposure to a β-amyloid protein fragment [25][26][27][28][29][30][31][32][33][34][35]. The AD disease model of neuronal cells was developed at cellular level using Aβ25-35 [149]. Polysaccharide fractions significantly inhibited the over accumulation of ROS induced by Aβ25-35 and the release of LDH and MDA, dependent on their intake [149].…”

Section: Antioxidant Activity Of Polysaccharidesmentioning

confidence: 99%

“…The AD disease model of neuronal cells was developed at cellular level using Aβ25-35 [149]. Polysaccharide fractions significantly inhibited the over accumulation of ROS induced by Aβ25-35 and the release of LDH and MDA, dependent on their intake [149]. FOAPs may also prevent cell apoptosis [149].…”

Section: Antioxidant Activity Of Polysaccharidesmentioning

confidence: 99%

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Natural Polysaccharides as Preventive and Therapeutic Horizon for Neurodegenerative Diseases

et al. 2021

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Neurodegenerative diseases are a serious and widespread global public health burden amongst aging populations. The total estimated worldwide global cost of dementia was US$818 billion in 2015 and has been projected to rise to 2 trillion US$ by 2030. While advances have been made to understand different neurodegenerative disease mechanisms, effective therapeutic strategies do not generally exist. Several drugs have been proposed in the last two decades for the treatment of different types of neurodegenerative diseases, with little therapeutic benefit, and often with severe adverse and side effects. Thus, the search for novel drugs with higher efficacy and fewer drawbacks is an ongoing challenge in the treatment of neurodegenerative disease. Several natural compounds including polysaccharides have demonstrated neuroprotective and even therapeutic effects. Natural polysaccharides are widely distributed in plants, animals, algae, bacterial and fungal species, and have received considerable attention for their wide-ranging bioactivity, including their antioxidant, anti-neuroinflammatory, anticholinesterase and anti-amyloidogenic effects. In this review, we summarize different mechanisms involved in neurodegenerative diseases and the neuroprotective effects of natural polysaccharides, highlighting their potential role in the prevention and therapy of neurodegenerative disease.

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Characterisation and identification of chemical constituents in aqueous extract of Fomes officinalis Ames based on ultrahigh‐performance liquid chromatography tandem quadrupole‐Orbitrap high‐resolution mass spectrometry

Zhang,

Aisa,

Liu

et al. 2023

Phytochemical Analysis

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IntroductionFungal species are an attractive resource for physiologically functional food and drug precursor. Fomes officinalis Ames, a medicinal fungus, is traditionally used as a folk medicine in traditional Chinese medicine prescription for the therapy of cough and asthma. The water‐soluble substances in Chinese herbal medicines are likely to play an important physiological function. However, information on probing and identifying chemical components of the aqueous extract of Fomes officinalis Ames (AFO) remains unknown.ObjectiveThis study was conducted to screen and characterise the chemical components of AFO.Material and MethodsAn effective and sensitive ultrahigh‐performance liquid chromatography tandem quadrupole‐Orbitrap high‐resolution mass spectrometry (UHPLC‐Q‐Orbitrap‐HRMS) method with the Full MS/PIL/dd‐MS2 acquisition approach was applied for the profiling of chemical components in AFO. An HSS T3 column was used for component separation, and a strategy of simultaneous targeted and untargeted multicomponent characterisation was implemented. Multiple identification approaches were used, including accurate molecular mass and elemental composition matching, literature and database searching, and fragmentation rules elucidation.ResultsA total of 115 components, including 20 amino acids and derivatives, six nucleobases, nine nucleosides, 75 dipeptides, two tripeptides, and three other components, were tentatively identified. Among them, the targeted exploring method screened six nucleobases and nine nucleosides including modified nucleosides. To our best knowledge, this is the first time a report has been done on the presence of the 115 compounds in AFO.ConclusionProfiling and characterisation compounds of AFO enriched its material basis, which would lay the foundation for improving potential medicinal and nutritional values and effecting comprehensive quality control of Fomes officinalis Ames.

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Neuroprotective effects of Fomes officinalis Ames polysaccharides on Aβ25–35-induced cytotoxicity in PC12 cells through suppression of mitochondria-mediated apoptotic pathway

Cited by 13 publications

References 24 publications

Protective Effect of Fucoidan against MPP+-Induced SH-SY5Y Cells Apoptosis by Affecting the PI3K/Akt Pathway

Protective Effect of Fucoidan against MPP+-Induced SH-SY5Y Cells Apoptosis by Affecting the PI3K/Akt Pathway

Natural Polysaccharides as Preventive and Therapeutic Horizon for Neurodegenerative Diseases

Characterisation and identification of chemical constituents in aqueous extract of Fomes officinalis Ames based on ultrahigh‐performance liquid chromatography tandem quadrupole‐Orbitrap high‐resolution mass spectrometry

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