Neuroprotective effects of corn silk maysin via inhibition of H2O2-induced apoptotic cell death in SK-N-MC cells

Choi, Doo Jin; Kim, Sun-Lim; Choi, Ji Won; Park, Yong Il

doi:10.1016/j.lfs.2014.05.020

Cited by 55 publications

(36 citation statements)

References 55 publications

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“…tive correlation (23). CAT is an enzyme scavenger that is able to remove hydrogen peroxide from the body to prevent cells from hydrogen peroxide-induced damage (24). Therefore, the results of the present study indicate that DCPT exerts strong antioxidative effects in vitro and induces effective oxidation resistance in vivo.…”

Section: Discussionmentioning

confidence: 53%

Antioxidant-mediated preventative effect of Dragon-pearl tea crude polyphenol extract on reserpine-induced gastric ulcers

Wang

Sun

2015

Experimental and Therapeutic Medicine

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Abstract. Dragon-pearl tea is a type of green tea commonly consumed in Southwest China. In the present study, the antioxidative and anti-gastric ulcer effects of Dragon-pearl tea crude polyphenols (DTCP) were determined in vitro and in vivo. Treatment with 25, 50 or 100 µg/ml DTCP resulted in notable antioxidant effects in vitro, which manifested as 2,2-diphenyl-1-picrylhydrazyl and OH radical-scavenging activity. Furthermore, using an in vivo mouse model, DTCP was shown to reduce the gastric ulcer area in the stomach, in which the 200 mg/kg DTCP dose exhibited the most marked effect, with a gastric ulcer index inhibitory rate of 72.63%. In addition, DTCP was demonstrated to improve stomach acidity conditions in vivo by increasing the pH and reducing the level of gastric juice, as compared with the reserpine-induced gastric ulcer control mice. Furthermore, DTCP altered the serum levels of a number of oxidation-related biomolecules, including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), lipid peroxidation (LPO) and catalase (CAT), to subsequently exert an anti-gastric ulcer effect. Treatment with 50, 100 and 200 mg/kg DTCP increased the SOD, GSH-Px and CAT levels and reduced the MDA and LPO levels in the mouse model of gastric ulcers. These serum level alterations resulted in the modified serum levels of prostaglandin E2 (PGE2) and nitric oxide (NO), which are associated with gastric mucosal protection. A reverse transcription-quantitative polymerase chain reaction (RT-PCR) assay is a molecular biology experiment which could determine the changes of mRNA in tissues. Using the RT-PCR assay, DTCP was observed to increase the mRNA expression levels of certain genes associated with gastric ulcers: Epidermal growth factor, epidermal growth factor receptor, vascular endothelial growth factor and vascular endothelial growth factor receptor 1, while reducing gastrin expression levels. Therefore, the results indicated that DTCP induced a marked preventative effect on reserpine-induced gastric ulcers in vivo, as a result of its antioxidative capacity.

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Section: Discussionmentioning

confidence: 53%

Antioxidant-mediated preventative effect of Dragon-pearl tea crude polyphenol extract on reserpine-induced gastric ulcers

Wang

Sun

2015

Experimental and Therapeutic Medicine

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“…As a typical ROS, hydrogen peroxide (H 2 O 2 ) is widely used as an apoptosis inducer16. It has been shown that H 2 O 2 induced DNA damage and apoptosis in human neuroblastoma SK-N-MC cells25. H 2 O 2 could also elicit Ca 2+ influx through store-operated channels (SOCs) in HEK-293 cells26.…”

Section: Discussionmentioning

confidence: 99%

The interplay between p16 serine phosphorylation and arginine methylation determines its function in modulating cellular apoptosis and senescence

Yang

et al. 2017

Sci Rep

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Cyclin-dependent kinase inhibitor p16INK4a (p16) primarily functions as a negative regulator of the retinoblastoma protein (Rb) -E2F pathway, thus plays critical role in cell cycle progression, cellular senescence and apoptosis. In this study, we showed that the methylation of Arg 138 and the phosphorylation of Ser 140 on p16 were critical for the control of cell proliferation and apoptosis. Compared to wild type p16, mutant p16R138K possessed improved function in preventing cell proliferation and inducing apoptosis, while the Ser 140 mutation (p16S140A) exhibited the opposite alteration. We also demonstrated that H2O2 was able to induce the phosphorylation of p16, which facilitated the interaction between CDK4 (Cyclin-dependent protein kinase) and p16, in 293T (human emborynic kidney) cells. Furthermore, the elevated arginine methylation in p16S140A mutant and increased serine phosphorylation in p16R138K mutant suggest that a antagonizing mechanism coordinating Arg 138 methylation and Ser 140 phosphorylation to regulates p16 function as well as cellular apoptosis and senescence. These findings will therefore contribute to therapeutic treatment for p16-related gene therapy by providing theoretical and experimental evidence.

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“…Among these compounds, maysin [rhamnosy-6-C-(4-ketofusyl)-5,7,3′ 4′ tetrahydroxyflavone], a flavonoid glycoside containing a rhamnose residue, is the most abundant flavonoid [22]. Maysin from corn silk has shown anticancer [23], neuroprotective [24], immunomodulating [23], and antiobesity activities [7]. The contour plot for maysin contents of NOV-treated UCSE as functions of NOV concentration, reaction temperature, and reaction time is shown in Figure 3.…”

Section: Effects Of Nov Treatment On Maysin Contentsmentioning

confidence: 99%

“…Corn silk has been used for the treatment of several diseases with multiple pharmacological activities reported, such as antioxidant [2], antidiabetic [3], antitumor [4], immune enhancement [5], antifatigue [6], antiobesity [7], and neuroprotective effects [8]. Heat reflux, organic solvent and ultrasound assistance are common methods for extracting polysaccharides from plant tissues; however, these processes generally require long extraction times, high extraction temperatures, and/or result in substantial environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

Enzymolysis-Assisted Treatments for Improved Phytochemical Contents and Bioactivities Levels from Unfertilized Corn Silk Extracts

Hs¹,

Sl²,

Hj³

et al. 2017

J Food Process Technol

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Neuroprotective effects of corn silk maysin via inhibition of H2O2-induced apoptotic cell death in SK-N-MC cells

Cited by 55 publications

References 55 publications

Antioxidant-mediated preventative effect of Dragon-pearl tea crude polyphenol extract on reserpine-induced gastric ulcers

Antioxidant-mediated preventative effect of Dragon-pearl tea crude polyphenol extract on reserpine-induced gastric ulcers

The interplay between p16 serine phosphorylation and arginine methylation determines its function in modulating cellular apoptosis and senescence

Enzymolysis-Assisted Treatments for Improved Phytochemical Contents and Bioactivities Levels from Unfertilized Corn Silk Extracts

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