Neuroprotective effects of carnosine-loaded elastic liposomes in cerebral ischemia rat model

Zeb, Alam; Cha, Ji-Hye; Noh, Ah Reum; Qureshi, Omer Salman; Kim, Kyoung-Won; Choe, Yeong-Hwan; Shin, Dong Bin; Shah, Fawad Ali; Majid, Arshad; Bae, Ok‐Nam; Kim, Jin‐Ki

doi:10.1007/s40005-019-00462-y

Cited by 16 publications

(13 citation statements)

References 48 publications

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“…Deformability is the most important parameter that allows for the internalization of vesicles across the biomembrane [ 42 ]. The special ability of elastic liposomes, including deformation and reformation, can be explained via the deformability index [ 43 ]. The relative deformability index of elastic liposomes (SC-EL and SGDC-EL) as compared with the 5% STC liposomes are listed in Table 2 .…”

Section: Discussionmentioning

confidence: 99%

Development, Characterization, and Ex Vivo Assessment of Elastic Liposomes for Enhancing the Buccal Delivery of Insulin

et al. 2021

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Buccal drug delivery is a suitable alternative to invasive routes of drug administration. The buccal administration of insulin for the management of diabetes has received substantial attention worldwide. The main aim of this study was to develop and characterize elastic liposomes and assess their permeability across porcine buccal tissues. Sodium-cholate-incorporated elastic liposomes (SC-EL) and sodium-glycodeoxycholate-incorporated elastic liposomes (SGDC-EL) were prepared using the thin-film hydration method. The prepared liposomes were characterized and their ex vivo permeability attributes were investigated. The distribution of the SC-EL and SGDC-EL across porcine buccal tissues was evaluated using confocal laser scanning microscopy (CLSM). The SGDC-EL were the most superior nanocarriers since they significantly enhanced the permeation of insulin across porcine buccal tissues, displaying a 4.33-fold increase in the permeability coefficient compared with the insulin solution. Compared with the SC-EL, the SGDC-EL were better at facilitating insulin permeability, with a 3.70-fold increase in the permeability coefficient across porcine buccal tissue. These findings were further corroborated based on bioimaging analysis using CLSM. SGDC-ELs showed the greatest fluorescence intensity in buccal tissues, as evidenced by the greater shift of fluorescence intensity toward the inner buccal tissue over time. The fluorescence intensity ranked as follows: SGDC-EL > SC-EL > FITC–insulin solution. Conclusively, this study highlighted the potential nanocarriers for enhancing the buccal permeability of insulin.

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Section: Discussionmentioning

confidence: 99%

Development, Characterization, and Ex Vivo Assessment of Elastic Liposomes for Enhancing the Buccal Delivery of Insulin

et al. 2021

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“…The presence of surfactant in NLC formulation could contribute to the enhanced membrane fluidity, stopping brain endothelial cell efflux pumps, as well as better attachment to lipoprotein receptors that support brain delivery [28]. Besides, NLCs transport across BBB could be mediated via endocytosis, transcytosis, or paracellular opening of tight junctions [1,92]. The combination of these mechanisms might lead to the enhanced brain uptake of temazepam-NLCs.…”

Section: Pharmacokinetics and Brain Distributionmentioning

confidence: 99%

Nanostructured Lipid Carriers to Mediate Brain Delivery of Temazepam: Design and In Vivo Study

et al. 2020

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The opposing effect of the blood–brain barrier against the delivery of most drugs warrants the need for an efficient brain targeted drug delivery system for the successful management of neurological disorders. Temazepam-loaded nanostructured lipid carriers (NLCs) have shown possibilities for enhancing bioavailability and brain targeting affinity after oral administration. This study aimed to investigate these properties for insomnia treatment. Temazepam-NLCs were prepared by the solvent injection method and optimized using a 42 full factorial design. The optimum formulation (NLC-1) consisted of; Compritol® 888 ATO (75 mg), oleic acid (25 mg), and Poloxamer® 407 (0.3 g), with an entrapment efficiency of 75.2 ± 0.1%. The average size, zeta potential, and polydispersity index were determined to be 306.6 ± 49.6 nm, −10.2 ± 0.3 mV, and 0.09 ± 0.10, respectively. Moreover, an in vitro release study showed that the optimized temazepam NLC-1 formulation had a sustained release profile. Scintigraphy images showed evident improvement in brain uptake for the oral 99mTc-temazepam NLC-1 formulation versus the 99mTc-temazepam suspension. Pharmacokinetic data revealed a significant increase in the relative bioavailability of 99mTc-temazepam NLC-1 formulation (292.7%), compared to that of oral 99mTc-temazepam suspension. Besides, the NLC formulation exhibited a distinct targeting affinity to rat brain. In conclusion, our results indicate that the developed temazepam NLC formulation can be considered as a potential nanocarrier for brain-mediated drug delivery in the out-patient management of insomnia.

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“…The deformability of the bilayer membranes decreased at high TW80 concentration (3 and 4 mg/mL), possibly because of the coexistence of mixed micelles with less flexible structures 38 . On the other hand, the complete absence of an edge activator in the bilayer membranes of CORM-2-CLs rendered them too rigid to penetrate small pores 29 . CORM-2-UDLs-T2 formulation (SPC:TW80 of 8:2), which had the highest deformability, was chosen as the optimized formulation and used in all further experiments.…”

Section: Discussionmentioning

confidence: 99%

“…The excess CORM-2-UDLs were removed from the grid, and then we added a drop of 1% phosphotungstic acid solution for negative staining. The negatively stained sample was properly dried at room temperature and analyzed with TEM at an accelerating voltage of 200 kV 29 .…”

Section: Methodsmentioning

confidence: 99%

CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery

Lee

Zeb

Kim

et al. 2020

Acta Pharmaceutica Sinica B

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The short release half-life of carbon monoxide (CO) is a major obstacle to the effective therapeutic use of carbon monoxide-releasing molecule-2 (CORM-2). The potential of CORM-2-entrapped ultradeformable liposomes (CORM-2-UDLs) to enhance the release half-life of CO and alleviate skin inflammation was investigated in the present study. CORM-2-UDLs were prepared by using soy phosphatidylcholine to form lipid bilayers and Tween 80 as an edge activator. The deformability of CORM-2-UDLs was measured and compared with that of conventional liposomes by passing formulations through a filter device at a constant pressure. The release profile of CO from CORM-2-UDLs was evaluated by myoglobin assay. In vitro and in vivo anti-inflammatory effects of CORM-2-UDLs were assessed in lipopolysaccharide-stimulated macrophages and TPA-induced ear edema model, respectively. The deformability of the optimized CORM-2-UDLs was 2.3 times higher than conventional liposomes. CORM-2-UDLs significantly prolonged the release half-life of CO from 30 s in a CORM-2 solution to 21.6 min. CORM-2-UDLs demonstrated in vitro anti-inflammatory activity by decreasing nitrite production and pro-inflammatory cytokine levels. Furthermore, CORM-2-UDLs successfully ameliorated skin inflammation by reducing ear edema, pathological scores, neutrophil accumulation, and inflammatory cytokines expression. The results demonstrate that CORM-2-UDLs could be used as promising therapeutics against acute skin inflammation.

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Neuroprotective effects of carnosine-loaded elastic liposomes in cerebral ischemia rat model

Cited by 16 publications

References 48 publications

Development, Characterization, and Ex Vivo Assessment of Elastic Liposomes for Enhancing the Buccal Delivery of Insulin

Development, Characterization, and Ex Vivo Assessment of Elastic Liposomes for Enhancing the Buccal Delivery of Insulin

Nanostructured Lipid Carriers to Mediate Brain Delivery of Temazepam: Design and In Vivo Study

CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery

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