Neuroprotective effects of anti-aging oriental medicine Lycium barbarum against β-amyloid peptide neurotoxicity

Yu, Man-Shan; Leung, Sarana Ka-Yan; Lai, Sau-Wan; Che, Chi‐Ming; Zee, S. Y.; So, Kwok-Fai; Yuen, Wai-Hung; Chang, Raymond Chuen‐Chung

doi:10.1016/j.exger.2005.06.010

Cited by 187 publications

(160 citation statements)

References 65 publications

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“…We have proved that the extract isolated from the fruits of F. lycii markedly attenuated Aß-induced apoptosis in cultured cortical neurons (19). Also, we have shown that Lycium barbarum extract was able to protect neurons against DTTinduced cell death (20).…”

Section: Introductionmentioning

confidence: 77%

Characterization of the effects of anti-aging medicine Fructus lycii on β-amyloid peptide neurotoxicity

Lai²,

Ho³

et al. 2007

Int J Mol Med

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Abstract. Alzheimer's disease (AD) is an age-related neurodegenerative disease. There are increasing lines of evidence showing that the molecular signaling pathways in aged cells are altered so that cells are susceptible to injury. We and other laboratories have demonstrated the significant involvement of double-stranded RNA-dependent protein kinase (PKR) in ß-amyloid (Aß) peptide neurotoxicity and in AD. Fructus lycii (the fruit of Lycium barbarum) has long been used in oriental medicine as an anti-aging agent. Our previous studies demonstrated that the aqueous extract isolated from L. barbarum exhibited significant protection on cultured neurons against harmful chemical toxins such as Aß and dithiothreitol. We also showed that the polysaccharidecontaining extract (LBP) from L. barbarum exhibited neuroprotective effects in the retina against ocular hypertension in a laser-induced glaucoma animal model. In this study, we aimed to investigate whether LBP can elicit neuroprotection to neurons stressed by Aß peptides. Furthermore, we planned to isolate and identify the neuroprotective agent from LBP using chromatographic methods. Our results showed that pretreatment of LBP effectively protected neurons against Aß-induced apoptosis by reducing the activity of both caspase-3 and -2, but not caspase-8 and -9. A new arabinogalactan-protein (LBP-III) was isolated from LBP and attenuated Aß peptide-activated caspase-3-like activity.LBP-III markedly reduced the phosphorylation of PKR triggered by Aß peptide. Since the phosphorylation state of PKR increased with age, reduction of its phosphorylation triggered by Aß peptide may implicate that LBP-III from Fructus lycii is a potential neuroprotective agent in AD. As herbal medicine has received increasing attention for the treatment of AD, our study will open a window for the development of a neuroprotective agent for anti-aging from Chinese medicine.

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Section: Introductionmentioning

confidence: 77%

Characterization of the effects of anti-aging medicine Fructus lycii on β-amyloid peptide neurotoxicity

Lai²,

Ho³

et al. 2007

Int J Mol Med

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“…The PBS (0.01 M, pH 7.4) was prepared from 0.1 M phosphate buffer (PB; pH 7.4), which was prepared from sodium phosphate dibasic dodecahydrate and sodium phosphate monobasic monohydrate (Sigma-Aldrich, St. Louis, MO, USA) and sodium chloride (Affymetrix USB, Brockville, Canada). The preparation of LBP powder was as previously described (56). The LBP powder was stored in a dry box and freshly dissolved in PBS before use.…”

Section: Animalsmentioning

confidence: 99%

“…In our laboratory, we use Lycium barbarum bought from the Ning Xia Huizu Autonomous Region, People's Republic of China. We have previously shown that the polysaccharides extracted from Lycium barbarum (LBP) reduced the death of cultured cortical neurons challenged by bamyloid, glutamate, and homocysteine (23,(54)(55)(56) and alleviated depression of rats by enhancing synaptic plasticity (57). LBP also delayed the degeneration of RGCs in the acute ischemia/reperfusion model (20,36), the chronic ocular hypertension model (5,35,37), and the mouse transient middle cerebral artery occlusion model (32).…”

Section: Introductionmentioning

confidence: 99%

Effect of Lycium barbarum (Wolfberry) on Alleviating Axonal Degeneration after Partial Optic Nerve Transection

Ruan

Kau

et al. 2015

Cell Transplant

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Our previous results showed that the polysaccharides extracted from Lycium barbarum (LBP) could delay secondary degeneration of retinal ganglion cell bodies and improve the function of the retinas after partial optic nerve transection (PONT). Although the common degeneration mechanisms were believed to be shared by both neuronal bodies and axons, recently published data from slow Wallerian degeneration mutant (Wld s ) mice supported the divergence in the mechanisms of them. Therefore, we want to determine if LBP could also delay the degeneration of axons after PONT. Microglia/macrophages were thought to be a source of reactive oxygen species after central nervous system (CNS) injury. After PONT, however, oxidative stress was believed to occur prior to the activation of microglia/macrophages in the areas vulnerable to secondary degeneration both in the optic nerves (ONs) and the retinas. But the results did not take into account the morphological changes of microglia/macrophages after their activation. So we examined the morphology in addition to the response magnitude of microglia/macrophages to determine their time point of activation. In addition, the effects of LBP on the activation of microglia/macrophages were investigated. The results showed that (1) LBP reduced the loss of axons in the central ONs and preserved the g-ratio (axon diameter/ fiber diameter) in the ventral ONs although no significant effect was detected in the dorsal ONs; (2) microglia/macrophages were activated in the ONs by 12 h after PONT; (3) LBP decreased the response magnitude of microglia/macrophages 4 weeks after PONT. In conclusion, our results showed that LBP could delay secondary degeneration of the axons, and LBP could also inhibit the activation of microglia/macrophages. Therefore, LBP could be a promising herbal medicine to delay secondary degeneration in the CNS via modulating the function of microglia/macrophages.

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“…It is an important risk factor of several neurodegenerative diseases including Alzheimer's disease (AD) (Yu et al, 2005). AD is a neurodegenerative disorder with increasing prevalence in the elderly population in the western world and the most common cause of age-related intellectual impairment occurring after the age of 60 (Silva et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Aging is one of the factors which results in the dysfunction of normal cellular regulation, affecting both central nervous and immune systems (Kawahami et al, 1999;Yu et al, 2005). It is an important risk factor of several neurodegenerative diseases including Alzheimer's disease (AD) (Yu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and acetylcholinesterase inhibitory activity of an isolated crinine alkaloid from Boophane disticha (Amaryllidaceae)

Adewusi

Fouché

Steenkamp

2012

Journal of Ethnopharmacology

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Ethnopharmacological relevance: Boophane disticha of the family Amaryllidaceae is used traditionally in southern Africa in the treatment of several neurological disorders.Aim of the study: Although acetylcholinesterase (AChE) inhibitory activity has been reported for this plant, the aim of the study was to identify and characterize the compound responsible for this activity using bioassay guided fractionation. Toxicity of the isolated compound was also assessed. Materials and methods:Bioassay guided isolation of the active compound from the methanol extract was carried out using column chromatography, TLC and preparative thin layer chromatography. Structural elucidation was carried out using high field 1D and 2D NMR and mass spectroscopy. AChE inhibitory activity was determined using the Ellman's colorimetric method. Cytotoxicity assessment was determined in human neuroblastoma (SH-SY5Y) cells using the MTT and neutral red uptake assays. Results:The data obtained from the integration of the 1 H spectra confirmed the compound to be a 3:1 mixture of two epimers, with epimer A, 6α-hydroxycrinamine as the major epimer. The IC 50 value for AChE inhibitory activity of the compound was 445 µM. The compound was observed to be cytotoxic in both the MTT and neutral red assays with IC 50 values of 54.5 and 61.7 µM, respectively. Conclusion:The present study describes for the first time, the isolation of 6-hydroxycrinamine, a crinine alkaloid, from the methanol extract of the bulbs of B. disticha. Although this compound possessed AChE inhibitory activity, it was found to be toxic to the neuroblastoma cells. 4Quantitative structure-activity relationship studies could be carried out to modify the structure in order to make it less toxic and improve its activity.

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Neuroprotective effects of anti-aging oriental medicine Lycium barbarum against β-amyloid peptide neurotoxicity

Cited by 187 publications

References 65 publications

Characterization of the effects of anti-aging medicine Fructus lycii on β-amyloid peptide neurotoxicity

Characterization of the effects of anti-aging medicine Fructus lycii on β-amyloid peptide neurotoxicity

Effect of Lycium barbarum (Wolfberry) on Alleviating Axonal Degeneration after Partial Optic Nerve Transection

Cytotoxicity and acetylcholinesterase inhibitory activity of an isolated crinine alkaloid from Boophane disticha (Amaryllidaceae)

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