Neuroprotective Effects of a Butanol Fraction of Rosa hybrida Petals in a Middle Cerebral Artery Occlusion Model

Abstract

The neuroprotective effects of a butanol fraction of white rose petal extract (WRPE-BF) were investigated in a middle cerebral artery occlusion (MCAO) model. Seven week-old male rats were orally administered WRPE-BF for 2 weeks and subjected to MCAO for 2 h, followed by reperfusion. Twenty-four h later, MCAO-induced behavioral dysfunctions were markedly improved in a dose-dependent manner by pretreatment with WRPE-BF. Moreover, higher dose of WRPE-BF not only decreased infarction area but also effectively reduced astrogliosis. The expression of inducible nitric oxide synthase, cyclooxygenase-2, and glial fibrillary acidic protein in MCAO model were markedly inhibited by WRPE-BF treatment. Notably, WRPE-BF decreased nitric oxide and malondialdehyde levels in the striatum and subventricular zone of stroke-challenged brains. These data suggested that WRPE-BF may exert its neuroprotective effects via anti-oxidative and anti-inflammatory activities against ischemia-reperfusion brain injury and could be a good candidate as a therapeutic target for ischemic stroke.

Rosa hybrida extract suppresses vascular smooth muscle cell responses by the targeting of signaling pathways, cell cycle regulation and matrix metalloproteinase-9 expression

“…A natural product such as WERH that targets PDGF responses in VSMCs is a potential therapeutic strategy in the prevention or inhibition of vascular diseases. The extract of Rosa hybrida is known to be used in the cosmetics industry (20), and it is also known to exert various pharmacological effects, including antioxidant, anti-inflammatory, antimicrobial and neuroprotective effects (20)(21)(22). However, the effect of Rosa hybrida extract on cardiovascular diseases has not yet been examined to the best of our knowledge.…”

“…Previously, researchers have identified gallic acid and volatiles (1-butanol, dodecyl acrylate and cyclododecane) as the main components of R. hybrida (21). Previous studies have established various pharmacological effects for the extract of R. hybrida: it has been noted for its antioxidant, antimicrobial, anti-inflammatory and neuroprotective effects (20)(21)(22). However, the inhibitory effect of water extract of Rosa hybrida (WERH) on vascular diseases has not previously been studied to the best of our knowledge.…”

“…Rosa hybrida is used in the cosmetics industry as a source of aromatic oils (20). Previously, researchers have identified gallic acid and volatiles (1-butanol, dodecyl acrylate and cyclododecane) as the main components of R. hybrida (21). Previous studies have established various pharmacological effects for the extract of R. hybrida: it has been noted for its antioxidant, antimicrobial, anti-inflammatory and neuroprotective effects (20)(21)(22).…”

Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction

“…Several studies have reported that inhibiting inflammatory processes that produce cyclooxygenase (COX) -2 (COX-2) and inducible nitric oxide synthase (iNOS) reduce the number of strokes in animal models [2]. Many pro-inflammation enzymes such as iNOS, COXs and xanthine oxidase, participate in oxidative injury during cerebral ischemia [3].…”

Extraction conditions of white rose petals for the inhibition of enzymes related to skin aging

“…We have reported that extracts of flowers from Rosa hybrida and Rosa rugosa exerted antiallergic effects in vitro cell culture system as well as in vivo systemic and local hypersensitivity models including atopic dermatitis [ 10 11 12 ]. Especially, n-hexane and butanol fractions of white rose petal extract (WRPE) were found to have strong antioxidative activity [ 13 14 ] so that substantially prevented brain infarction in an ischemia-reperfusion model of stroke [ 15 ].…”