Neuroprotective effect of Tinospora cordifolia ethanol extract on 6-hydroxy dopamine induced Parkinsonism

Kosaraju, Jayasankar; Chinni, Santhivardhan; Roy, Priyankur; Elango, K; Antony, A Shanish; Kumar, M N Satish

doi:10.4103/0253-7613.129312

Cited by 87 publications

(40 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…T . cordifolia ethanol extract exhibited significant neuroprotection by increasing the dopamine levels (1.96 ± 0.20 and 2.45 ± 0.40 ng/mg of protein) and complex I activity (77.14 ± 0.89 and 78.50 ± 0.96 nmol/min/mg of protein) at 200 and 400 mg/kg, respectively [ 87 ]. Combination of ethanolic extracts of T .…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Genus Tinospora: Ethnopharmacology, Phytochemistry, and Pharmacology

Chi

She

Han

et al. 2016

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

The genus Tinospora includes 34 species, in which several herbs were used as traditional medicines by indigenous groups throughout the tropical and subtropical parts of Asia, Africa, and Australia. The extensive literature survey revealed Tinospora species to be a group of important medicinal plants used for the ethnomedical treatment of colds, headaches, pharyngitis, fever, diarrhea, oral ulcer, diabetes, digestive disorder, and rheumatoid arthritis. Indian ethnopharmacological data points to the therapeutic potential of the T. cordifolia for the treatment of diabetic conditions. While Tinospora species are confusing in individual ingredients and their mechanisms of action, the ethnopharmacological history of those plants indicated that they exhibit antidiabetic, antioxidation, antitumor, anti-inflammation, antimicrobial, antiosteoporosis, and immunostimulation activities. While the clinical applications in modern medicine are lacking convincing evidence and support, this review is aimed at summarizing the current knowledge of the traditional uses, phytochemistry, biological activities, and toxicities of the genus Tinospora to reveal its therapeutic potentials and gaps, offering opportunities for future researches.

show abstract

Section: Pharmacological Activitiesmentioning

confidence: 99%

Genus Tinospora: Ethnopharmacology, Phytochemistry, and Pharmacology

Chi

She

Han

et al. 2016

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…However, neuroprotective activity of this plant is least explored. Recently, the ethanolic extract of T. cordifolia was reported to exhibit neuroprotective activity against 6-OHDA induced Parkinsonism [ 15 ]. Recent studies from our lab have reported that 50% aqueous ethanolic extract of T. cordifolia (TCE) ameliorated anxiety, improved exploratory behavior and modulated synaptic plasticity in sleep-deprived rats [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Tinospora cordifolia as a potential neuroregenerative candidate against glutamate induced excitotoxicity: an in vitro perspective

Sharma

Kaur

2018

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundGlutamate, the major excitatory neurotransmitter of CNS acts as a neurotoxin at higher concentrations. Prolonged activation of glutamate receptors results in progressive neuronal damage by aggravating calcium influx, inducing mitochondrial damage and oxidative stress. Excitotoxic cell death is associated with the pathogenesis of various neurodegenerative disorders such as trauma, brain injury and neurodegenerative diseases. The current study was designed to investigate the neuroprotective and neuroregenerative potential of Tinospora cordifolia against glutamate-induced excitotoxicity using primary cerebellar neuronal cultures as a model system.MethodsMonosodium salt of glutamate was used to induce neurotoxic injury in primary cerebellar neurons. Four extracts including Hexane extract, Chloroform extract, Ethyl acetate, and Butanol extract were obtained from fractionation of previously reported aqueous ethanolic extract of T. cordifolia and tested for neuroprotective activity. Out of the four fractions, Butanol extract of T. cordifolia (B-TCE) exhibited neuroprotective potential by preventing degeneration of neurons induced by glutamate. Expression of different neuronal, apoptotic, inflammatory, cell cycle regulatory and plasticity markers was studied by immunostaining and Western blotting. Neurite outgrowth and migration were also studied using primary explant cultures, wound scratch and gelatin zymogram assay.ResultsAt molecular level, B-TCE pretreatment of glutamate-treated cultures normalized the stress-induced downregulation in the expression of neuronal markers (MAP-2, GAP-43, NF200) and anti-apoptotic marker (Bcl-xL). Further, cells exposed to glutamate showed enhanced expression of inflammatory (NF-κB, AP-1) and senescence markers (HSP70, Mortalin) as well as the extent of mitochondrial damage. However, B-TCE pretreatment prevented this increase and inhibited glutamate-induced onset of inflammation, stress and mitochondrial membrane damage. Furthermore, B-TCE was observed to promote regeneration, migration and plasticity of cerebellar neurons, which was otherwise significantly inhibited by glutamate treatment.ConclusionThese results suggest that B-TCE may have neuroprotective and neuroregenerative potential against catastrophic consequences of glutamate-mediated excitotoxicity and could be a potential therapeutic candidate for neurodegenerative diseases.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2330-6) contains supplementary material, which is available to authorized users.

show abstract

“…Along with its radio- and chemoprotective effects, T. cordifolia has also been reported to show neuroprotective potential by modulating anti-oxidant enzyme system of brain tissue 10 . Oral dose of ethanolic extract of T. cordifolia has been found to enhance the dopamine level and complex-I activity in 6-hydroxy dopamine (6-OHDA) induced Parkinson model of rat along with reduced oxidative stress and improved locomotor activity 11 . Petroleum ether extract of T. cordifolia has been reported to reverse the depression-like behavior in mice and reduced the monoamine oxidase activities in the brain, resulting in increased levels of brain monoamines 12 .…”

mentioning

confidence: 99%

Tinospora cordifolia ameliorates anxiety-like behavior and improves cognitive functions in acute sleep deprived rats

Mishra

Manchanda

Gupta

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Sleep deprivation (SD) leads to the spectrum of mood disorders like anxiety, cognitive dysfunctions and motor coordination impairment in many individuals. However, there is no effective pharmacological remedy to negate the effects of SD. The current study examined whether 50% ethanolic extract of Tinospora cordifolia (TCE) can attenuate these negative effects of SD. Three groups of adult Wistar female rats - (1) vehicle treated-sleep undisturbed (VUD), (2) vehicle treated-sleep deprived (VSD) and (3) TCE treated-sleep deprived (TSD) animals were tested behaviorally for cognitive functions, anxiety and motor coordination. TSD animals showed improved behavioral response in EPM and NOR tests for anxiety and cognitive functions, respectively as compared to VSD animals. TCE pretreatment modulated the stress induced-expression of plasticity markers PSA-NCAM, NCAM and GAP-43 along with proteins involved in the maintenance of LTP i.e., CamKII-α and calcineurin (CaN) in hippocampus and PC regions of the brain. Interestingly, contrary to VSD animals, TSD animals showed downregulated expression of inflammatory markers such as CD11b/c, MHC-1 and cytokines along with inhibition of apoptotic markers. This data suggests that TCE alone or in combination with other memory enhancing agents may help in managing sleep deprivation associated stress and improving cognitive functions.

show abstract

Neuroprotective effect of Tinospora cordifolia ethanol extract on 6-hydroxy dopamine induced Parkinsonism

Cited by 87 publications

References 16 publications

Genus Tinospora: Ethnopharmacology, Phytochemistry, and Pharmacology

Genus Tinospora: Ethnopharmacology, Phytochemistry, and Pharmacology

Tinospora cordifolia as a potential neuroregenerative candidate against glutamate induced excitotoxicity: an in vitro perspective

Tinospora cordifolia ameliorates anxiety-like behavior and improves cognitive functions in acute sleep deprived rats

Contact Info

Product

Resources

About