Neuroprotective Effect of Resveratrol on Acute Brain Ischemia Reperfusion Injury by Measuring Annexin V, p53, Bcl-2 Levels in Rats

Abstract

BackgroundCerebral ischemia is as a result of insufficient cerebral blood flow for cerebral metabolic functions. Resveratrol is a natural phytoalexin that can be extracted from grape's skin and had potent role in treating the cerebral ischemia. Apoptosis, a genetically programmed cellular event which occurs after ischemia and leads to biochemical and morphological changes in cells. There are some useful markers for apoptosis like Bcl-2, bax, and p53. The last reports, researchers verify the apoptosis with early markers like Annexin V.MethodsWe preferred in this experimental study a model of global cerebral infarction which was induced by bilateral common carotid artery occlusion method. Rats were randomly divided into 4 groups : sham, ischemia-reperfusion (I/R), I/R plus 20 mg/kg resveratrol and I/R plus 40 mg/kg resveratrol. Statistical analysis was performed using Sigmastat 3.5 ve IBM SPSS Statistics 20. We considered a result significant when p<0.001.ResultsAfter administration of resveratrol, Bcl-2 and Annexin levels were significantly increased (p<0.001). Depending on the dose of resveratrol, Bcl2 levels increased, p53 levels decreased but Annexin V did not effected. P53 levels were significantly increased in ishemia group, so apoptosis is higher compared to other groups.ConclusionIn the acute period, Annexin V levels misleading us because the apoptotic cell counts could not reach a certain level. Therefore we should support our results with bcl-2 and p53.

Apurinic/apyrimidinic endonuclease 1 (APE1) contributes to resveratrol-induced neuroprotection against oxygen-glucose deprivation and re-oxygenation injury in HT22 cells: Involvement in reducing oxidative DNA damage

“…Resveratrol, a stilbene formed in many plants in response to various stressors, elicits multiple beneficial effects including anti-oxidative, anti-apoptotic and anti-inflammatory properties in dozens of diseases (28). Particularly, resveratrol was shown to have therapeutic properties in neuronal following ischemia reperfusion injury (29,30). Resveratrol post-treatment protects against neonatal brain injury after hypoxia-ischemia (20).…”

The GABAergic system as a therapeutic target for Alzheimer's disease

“…Resveratrol (3,5,4 0 -trihydroxy-trans-stilbene) is a plantbased phytoalexin with antioxidant, anti-inflammatory, antidiabetic, and anti-aging properties (Kizmazoglu et al 2015). This compound, together with its dimer trans-e-viniferin, may improve cognition by modulating GABA A R-induced currents (Moussa et al 2017).…”

Identification of novel biomarkers for prediction of neurological prognosis following cardiac arrest

“…Cerebral ischemia is a result of insufficient cerebral blood flow for cerebral metabolic functions. Reperfusion stimulates many pathological mechanisms such as leukocyte infiltration, oxidative stress, inflammation, destruction of blood-brain barrier, platelet activation, nitric oxide release, and apoptosis [19]. …”

“…They are divided on two distinct groups, anti-apoptotic members such as Bcl-2 and Bcl-Xl that prevent cell death and pro-apoptotic members like Bax and Bak [30, 31]. The animal studies of brain ischemia-reperfusion injury showed that the level of Bcl-2 was more decreased in severe brain injury [19, 32]. Like these findings from other studies, our results show that the gene expression of anti-apoptotic protein, Bcl-2 showed greater decreases in poor neurological group compared with good neurological group.…”