Neuroprotective Effect of Pedalium murex Linn. Leaf against Lipopolysaccharide Induced Behavioural Disorders in Rats

Abstract

1Endotoxic shock can be elicited by a systemic injection of LPS which induces the production and release of several cytokines.2 In response to these cytokines, several reactive oxygen species (ROS) are produced from cells such as neutrophils and other phagocytic cells, creating a status of oxidative stress.3 Thus, this type of stress may hypothetically support the assumption that LPSinduced cell injury would be retarded by modifying free radical metabolism with the aid of potent antioxidant pre-treatment from nature. Certain reports have claimed that a few herbal extracts can act on the central nervous system, thereby enhancing the faculties of learning and memory. 4 The . At the end of the study, rats were sacrificed, brain hippocampal region was removed and biochemical parameters were measured. Results: In WMT swimming length (cm) was increased in LPS-treated rats when compared to control animals, the swimming length (EEPM; 400 mg/kg) was found to be significant; in RAM, different doses of EEPM at 100, 200 and 400 mg/kg decreased the number of errors in entry 4.00±0.36, 4.16±0.16and 3.33±2.79 respectively when compared with control animals (2.66± 0.21). EEPM at 400 mg/kg showed significant activity, in CRT apparatus increased incorrect lever pressing was observed in LPS-treated rats when compared to control animals. Incorrect lever pressing was minimized by EEPM at 400 mg/kg (43.5±2.40). Conclusions: Our results showed that EEPM is a hopeful aspirant for hindrance of infection and inflammation induced brain damage by LPS.

Neuroprotective Effect of Ethanolic Extract of Pedalium murex Linn Leaf in 3-Nitropropionic acid Induced Neurodegeneration

“…Hence, the present study was carried at two dose levels, i.e., at 200 and 400 mg/kg body weight. To prepare the test drug, required quantity of the EEPML was dissolved in distilled water to have a desired dose in 1mL solution [20]. 3-NP was dissolved in 5% dimethyl sulfoxide saline (pH 7.4) [21].…”

“…On day 14, group II to V received an injection of 3-Nitropropionic acid (10 mg/kg/i.p.) in normal saline to cause neurodegeneration [20]. Animals were given behavioural tests two hours after receiving 3-Nitropropionic acid, and then euthanised by mild halogenated ether anesthesia and the brain was removed for biochemical study.…”

Neuroprotective effect of ethanolic leaf extract of Commiphora caudata (Wight & Arn) against lipopolysaccharide-induced neurotoxicity in Wistar rats

“…A radial arm maze is a method to assess the working memory of animals (21). It consists of a wooden apparatus with eight elevated-arm radial maze, which extends from the central platform having a diameter of 26 cm.…”