Neuroprotective effect of osthole against oxygen and glucose deprivation in rat cortical neurons: involvement of mitogen-activated protein kinase pathway

Chen, T.; Liu, W.; Chao, Xiaodong; Qu, Yan; Zhang, L.; Luo, Peng; Xie, Keliang; Huo, Jianhua; Fei, Z.

doi:10.1016/j.neuroscience.2011.03.038

Cited by 94 publications

(56 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…35,60) A volume of interesting studies have revealed the neuroprotective effects of Ost on some experimental models of cerebral ischemia/reperfusion and brain injury via anti-oxidative and anti-inflammatory activities. 33,35,60) Also Ost attenuated clinical severity and CNS inflammation and demyelination of EAE mice by blocking reduction of NGF and suppressed IFN-γ increase. 36) In spite of the large volume of known activities of Ost, its effect on adult hippocampal neurogenesis is still remained unknown.…”

Section: Discussionmentioning

confidence: 99%

“…After 7-14 d of culture, NSCs changed morphology and developed markers of neurons, astrocytes and oligodendroglia as verified by immunocytochemistry staining. 7,10) Cell Viability and Proliferation Assays Single NSCs at 5th passage were seeded at 5.0×10 4 cells/mL in proliferation media and exposed to various concentrations of Ost (0, 10, 50, and 100 µM) (34)(35)(36). After 3 d of co-culture, cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) assay as previously described.…”

Section: Preparation Of Ostmentioning

confidence: 99%

“…110822-200305; Beijing, China), dissolved in 0.05% carboxymethyl cellulose sodium (CMC-Na) and stored at 4°C. [34][35][36] Generation and Characterization of Adult NSCs Adult NSCs were isolated and expanded from the SVZ region of adult naïve C57BL/6 mice and cultured in NSC proliferation media as described in our laboratory. 7) Briefly, C57BL/6 mice at 4-6 weeks of age were euthanized; the SVZ regions of the fresh brain were harvested and cut into 1 mm 3 pieces, then suspended in 2 mL 0.25% Trypsin with ethylenediaminetetraacetic acid (EDTA) (Invitrogen), mechanically dissociated for 2 min and incubated at 37°C for 30 min.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Coumarin Derivative Osthole Stimulates Adult Neural Stem Cells, Promotes Neurogenesis in the Hippocampus, and Ameliorates Cognitive Impairment in APP/PS1 Transgenic Mice

Kong

Yao

et al. 2015

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

It is believed that neuronal death caused by abnormal deposition of amyloid-beta peptide is the major cause of the cognitive decline in Alzheimer's disease. Adult neurogenesis plays a key role in the rescue of impaired neurons and amelioration of cognitive impairment. In the present study, we demonstrated that osthole, a natural coumarin derivative, was capable of promoting neuronal stem cell (NSC) survival and inducing NSC proliferation in vitro. In osthole-treated APP/PS1 transgenic mice, a significant improvement in learning and memory function was seen, which was associated with a significant increase in the number of new neurons (Ki67 /NF-M ) and a decrease in apoptotic cells in the hippocampal region of the brain. These observations suggested that osthole promoted NSC proliferation, supported neurogenesis, and thus efficiently rescued impaired neurons in the hippocampus and ameliorated cognitive impairment. We also found that osthole treatment activated the Notch pathway and upregulated the expression of self-renewal genes Notch 1 and Hes 1 mRNA in NSCs. However, when Notch activity was blocked by the γ-secretase inhibitor DAPT, the augmentation of Notch 1 and Hes 1 protein was ameliorated, and the proliferation-inducing effect of osthole was abolished, suggesting that the effects of osthole are at least in part mediated by activation of the Notch pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Ostmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

The Coumarin Derivative Osthole Stimulates Adult Neural Stem Cells, Promotes Neurogenesis in the Hippocampus, and Ameliorates Cognitive Impairment in APP/PS1 Transgenic Mice

Kong

Yao

et al. 2015

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…19) A recent study also showed that osthole played a role in protecting neural cells against oxygen and glucose deprivation in rat cortical neurons, through regulating the expression of mitogen-activated protein kinase (MAPK) proteins. 20) However, the effect of osthole on Aβ-induced neurotoxicity in neural cells has been rarely reported so far. To gain further insight into the biological role (s) of osthole, in this study we attempted to assess the neuroprotective effects of osthole on a cell model of AD induced by exogenous Aβ and elucidate the correlationship between neuroprotection and CREB phosphorylation.…”

Section: )mentioning

confidence: 99%

“…After 10 d's culture, neurons were exposed to Aβ [25][26][27][28][29][30][31][32][33][34][35] at different concentrations (10,20,30,40 and 50 µM) for 24 h, then MTT assay was used to determine the cell viability. Results showed that Aβ [25][26][27][28][29][30][31][32][33][34][35] insult decreased cell survival in a concentration dependent manner (Fig.…”

Section: Osthole Reduced Intracellular Aβ Levels In Neural Cellsmentioning

confidence: 99%

Osthole Reverses Beta-Amyloid Peptide Cytotoxicity on Neural Cells by Enhancing Cyclic AMP Response Element-Binding Protein Phosphorylation

Wen

Liang

et al. 2013

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Accumulation of β-amyloid peptide (Aβ) in the brain plays an important role in the pathogenesis of Alzheimer's disease (AD). Previous studies have demonstrated the neuroprotective role of osthole against oxygen and glucose deprivation in cortical neurons. However, the effects of osthole on Aβ-induced neurotoxicity in neural cells have rarely been reported. The current study was designed to investigate the protective effects of osthole on a cell model of AD insulted by exogenous Aβ 25-35 and the potential mechanism(s). In this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, immunofluorescence analysis, apoptosis assay, reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) techniques were used in primary cortical neurons and SH-SY5Y cells. Our data showed that osthole reduced intracellular Aβ levels in neural cells, which was associated with decreased BACE1 protein; osthole reversed exogenous Aβ 25-35 -induced cell viability loss, apoptosis, and synapsin-1 reduction, which was related to the reestablishment of phosphorylation of cyclic AMP response element-binding protein (CREB). The collective evidence indicates that osthole possesses the ability to protect cortical neurons and SH-SY5Y cells against Aβ injury, and the underlying mechanism may be attributed to the enhancement of CREB phosphorylation.Key words osthole; Alzheimer's disease; cyclic AMP response element-binding protein; β-amyloid peptide [25][26][27][28][29][30][31][32][33][34][35] Alzheimer's disease (AD) is an irreversible neurodegenerative disorder of the brain characterized by progressive cognitive loss and memory decline. The abnormal deposition of β-amyloid peptide (Aβ) is one of the most notable neuropathological hallmarks of AD. Aβ-Induced synaptic loss and subsequent neuronal death have been implicated as a major cause of the cognitive decline associated with AD.1-4) Accumulating evidence suggests that multiple cellular targets and signaling pathways mediate Aβ-induced neurotoxicity, including N-methyl-D-aspartate (NMDA) receptors and cyclic AMP response element-binding protein (CREB).5,6) CREB is a prosurvival transcription factor responsible for the expression of a large number of downstream genes, 7) such as brain-derived neurotrophic factor (BDNF), an important neurotrophic factor that possessed ability to promote neural protection, plasticity, and regeneration in a variety of conditions, such as central nervous system (CNS) injury, cerebral ischemia, and electrical stimulation.8-10) It is well established that several protein kinase pathways converge to co-regulate CREB activity. Aβ has been shown to interfere with events downstream of activated CREB, especially gene expression of BDNF.11) Moreover, reduced phosphorylation of CREB (p-CREB) has been observed in postmortem brains of AD patients and in transgenic (Tg)-AD mice overexpressing Aβ.12,13) It has been reported that enhanced CREB function in the CA1 region of the dorsal hippocampus rescues the spatia...

show abstract

A rapid and sensitive LC‐MS/MS method for the determination of osthole in rat plasma: application to pharmacokinetic study

Fei

Shan

et al. 2013

Biomedical Chromatography

View full text Add to dashboard Cite

Osthole, a major component isolated from the fruit of Cnidium monnieri (L.) Cusson, has been widely used in traditional Chinese medicine. We developed and validated a rapid and sensitive LC-MS/MS method for the quantification of osthole in rat plasma. Sample preparation involved simple liquid-liquid extraction by ethyl acetate after addition of imperatorin as internal standard (IS). The analyte was separated using a C(18) column with the mobile phase of methanol-0.1% formic acid (80:20, v/v) at a flow rate of 0.4 mL/min. The elutes were detected under positive electrospray ionization in multiple reaction monitoring mode. The method was sensitive with 0.5 ng/mL as the lower limit of detection. Good linearity was obtained over the range of 1.0-500.0 ng/mL. The intra and inter-batch accuracy for osthole in rat plasma samples ranged from 99.5 to 108.1% and the variation was <8.9%. The stability, extraction efficiency and matrix effect were also acceptable. This method was successfully applied to the pharmacokinetic study of osthole in rat after intravenous and oral administration.

show abstract

Neuroprotective effect of osthole against oxygen and glucose deprivation in rat cortical neurons: involvement of mitogen-activated protein kinase pathway

Cited by 94 publications

References 38 publications

The Coumarin Derivative Osthole Stimulates Adult Neural Stem Cells, Promotes Neurogenesis in the Hippocampus, and Ameliorates Cognitive Impairment in APP/PS1 Transgenic Mice

The Coumarin Derivative Osthole Stimulates Adult Neural Stem Cells, Promotes Neurogenesis in the Hippocampus, and Ameliorates Cognitive Impairment in APP/PS1 Transgenic Mice

Osthole Reverses Beta-Amyloid Peptide Cytotoxicity on Neural Cells by Enhancing Cyclic AMP Response Element-Binding Protein Phosphorylation

A rapid and sensitive LC‐MS/MS method for the determination of osthole in rat plasma: application to pharmacokinetic study

Contact Info

Product

Resources

About