Neuroprotective Effect ofBrassica oleraceaSprouts Crude Juice in a Cellular Model of Alzheimer’s Disease

Abstract

β-Amyloid peptide (Aβ) aberrant production and aggregation are major factors implicated in the pathogenesis of Alzheimer's disease (AD), causing neuronal death via oxidative stress. Several studies have highlighted the importance of polyphenolic antioxidant compounds in the treatment of AD, but complex food matrices, characterized by a different relative content of these phytochemicals, have been neglected. In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25–35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions. Both juices protected against Aβ-induced cytotoxicity and apoptotic cell death as evidenced by cell viability, nuclear chromatin condensation, and apoptotic body formation measurements. These effects were mediated by the modulation of the mitochondrial function and of the HSP70 gene transcription and expression. Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and NAD(P)H:quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2). Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

Multiple mechanisms of dimethyl fumarate in amyloid β‐induced neurotoxicity in human neuronal cells

“…Newly, it was identified that insufficient Nrf2 activation in humans is linked to chronic diseases such as PD [31,69,70], AD [71,72] and amyotrophic lateral sclerosis [73]. Our findings provide elucidation of the molecular mechanisms involved in the neuroprotective action of DMF, evidencing the importance of the cell survival signalling pathways regulated by Nrf2 in term of oxidative stress.…”

Nutraceutical Improvement Increases the Protective Activity of Broccoli Sprout Juice in a Human Intestinal Cell Model of Gut Inflammation

“…In addition, we report that juices produced from elicited sprouts were more effective in providing cell protection. Interestingly, broccoli sprout juices, prepared under the same controlled conditions used in this study, were also shown to be protective in a cellular model of Alzheimer’s disease and in Spontaneously Hypertensive Stroke Prone rats [ 65 , 66 ].…”

Functional Ingredients From Brassicaceae Species: Overview and Perspectives

“…Other authors have evaluated different biological activities of Brassicaceae juices. Broccoli sprout juice has been studied as a potential therapeutic strategy for Alzheimer's disease [92]. A broccoli-cabbage juice was also efficient as a lowering LDL-cholesterol agent [93].…”