Neuroprotective effect of <i>Annona muricata</i>-derived polysaccharides in neuronal HT22 cell damage induced by hydrogen peroxide

Kim, Woo Sik; Kim, Yi-Eun; Cho, Eun-Ji; Byun, Eui‐Baek; Park, Woo‐Yong; Song, Ha-Yeon; Park, Sang‐Hyun; Byun, Eui-Hong

doi:10.1080/09168451.2020.1715201

Cited by 18 publications

(15 citation statements)

References 44 publications

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“…Similarly, there are studies on the content of alkaloids and vanoids with strong antioxidant effects [20,39]. In this study, we investigated the radioprotective effect of AME as an agent shown to have anticarcinogenic, anticonvulsant, antiin ammatory, antioxidant, radioprotective (against Gamma-ray) and neuroprotective properties [10,22,28]. There are only a few studies on the radioprotective effect of AM in the literature [10,17,21].…”

Section: Discussionmentioning

confidence: 99%

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The Neuroprotective and Anti-Inflammatory Effects of Annona Muricata (Graviola) on Radiation-Induced Rat Sciatic Nerve Injury.

Keskin¹,

Elmas²,

Şahin³

et al. 2021

Preprint

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Background: In radiotherapy (RT) exposure area, normal tissues are also affected that may cause serious complications in the patients. This study aimed to evaluate Annona muricata’s radioprotective effects on sciatic nerve injury due to ionizing radiation (IR). Methods and Results: 32 adult female Wistar albino rats separated into 4 equal groups; Control (C), Annona muricata leaf extracts (AME), radiation (RAD), radiation and Annona muricata leaf extracts (AME+RAD). In groups AME and AME+RAD, Annona muricata leaf extracts were administered at a dose of 300 mg/kg for the first day and 50 mg/kg everyday for following one week intraperitoneally. In RAD and AME+RAD, rats were exposed to a single dose of 20 Gray IR to their right legs. All the subjects were sacrified at the end of the first month. Oxidative stress biochemical parameters (SOD, CAT and GPx) from blood samples were analyzed. Right sciatic nerves extracted and histomorphology evaluated. Statistically significant vasculature, degenerative and necrotic changes were observed in RAD, compared to C and AME (p<0,01). Swelling in myelin sheath was predominantly seen in RAD. Alterations in the level of CAT (p<0,01), SOD (p<0,01) and GPx (p<0,05) in AME+RAD group compared to RAD group were found to be statistically significant.Conclusion: Our study unveiled that AM could have a potential of biochemically and histomorphology healing on sciatic nerve injury due to ionizing radiation.

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Section: Discussionmentioning

confidence: 99%

“…The rst defense mechanism against these harmful effects due to oxidative stress is provided by SOD. SOD neutralizes ROS to the less harmful compound hydrogen peroxide (H 2 O 2 ) [22]. However, excessive cellular H 2 O 2 accumulation increases oxidative damage by causing the formation of reactive free hydroxyl (OH-) radical [34,43].…”

Section: Discussionmentioning

confidence: 99%

The Neuroprotective and Anti-Inflammatory Effects of Annona Muricata (Graviola) on Radiation-Induced Rat Sciatic Nerve Injury.

Keskin¹,

Elmas²,

Şahin³

et al. 2021

Preprint

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“…In addition, AM, which contains many flavonoids, alkaloids and acetogenins in its leaves, is also used to manage infections, diarrhoea, dermatitis, diabetes, fever and cardiovascular problems [18]. In support of this traditional use, various studies have shown the anticancer, antioxidant, antiviral, antihaemolytic, sedative and neuroprotective effects of AM [19,20]. Radiation-induced peripheral nerve damage (RIPND) is permanent since nerve cells do not have the regeneration ability [21,22].…”

Section: Marmara Medical Journalmentioning

confidence: 99%

The neuroprotective and anti-inflammatory effects of Annona muricata (Graviola) on radiation-induced rat sciatic nerve injury

KESKIN¹,

ELMAS²,

SAHIN³

et al. 2022

Marmara Medical Journal

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Objective: This study aimed to evaluate Annona muricata's (AM) radioprotective effects on sciatic nerve injury due to ionising radiation (IR). Materials and Methods: Thirty-two adult female Wistar albino rats were separated into four equal groups; Control (C), Annona muricata leaf extracts (AME), radiation (RAD), radiation and AME (AME+RAD). In groups AME and AME+RAD, AME was administered at a dose of 300 mg/kg for the first day and 50 mg/every day for the following one week intraperitoneally. In RAD and AME+RAD, rats were exposed to a single dose of 20 Gray (Gy) IR to their right legs. All the subjects were sacrificed at the end of the first month. Oxidative stress biochemical parameters from blood samples were analysed. In addition, right sciatic nerves were extracted and histomorphology was evaluated. Results: Statistically significant vasculature, degenerative and necrotic changes were observed in RAD, compared to C and AME (p<0.01). Swelling in myelin sheath was predominantly seen in RAD. Alterations in the level of catalase (p<0.01), superoxide dismutase (p<0.01) and glutathione peroxidase (p<0.05) in the AME+RAD group compared to the RAD group were found to be statistically significant. Conclusion: Our study unveiled that AM could potentially enhance biochemical and histomorphological healing in the acute period on sciatic nerve injury due to IR.

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“…Our studies have also shown that galactose (68.4%) is the major monosaccharide among the A. muricata leaf polysaccharides (ALPS), followed by glucose (24.37%), mannose (9.81%), and other sugars. ALPS exert protective effects against oxidative stress-induced cellular damage and radiation-induced skin injury ( 20 , 21 ). However, there is no evidence supporting the cytoprotective role of ALPS against chemotherapy-induced toxicity.…”

Section: Introductionmentioning

confidence: 99%

Polysaccharides from Annona muricata leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction

et al. 2022

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cisplatin is a prominent chemotherapeutic agent that can induce significant damage to normal cells. Therefore, it is important to develop agents that protect normal cells without influencing the chemotherapeutic effect of cisplatin. The present study was conducted to explore the protective effects of Annona muricata leaf polysaccharides (ALPS) against cisplatin-induced toxicity in macrophages. Apoptosis was assessed in macrophages and lung cancer cells to investigate the cytoprotective effect of ALPS, their effect on the production of cisplatin-induced reactive oxygen species (ROS) and the loss of the mitochondrial transmembrane potential (MTP). Cisplatin, when used alone or in combination with ALPS, showed significant toxicity against A549 and H460 lung cancer cells. However, cisplatin-induced cytotoxicity was suppressed by cotreatment of RAW 264.7 macrophages with ALPS. ALPS significantly inhibited the upregulation of Bax, cytosolic cytochrome c and caspases-3, -8 and -9. Moreover, ALPS resulted in the cleavage of PARP and downregulation of Bcl-2 levels in a concentration-dependent manner, which ultimately led to a reduction in the apoptotic and necrotic populations of cisplatin-treated RAW 264.7 macrophages. The suppression of the apoptotic signaling pathways was mediated through the reduction of ROS and MTP loss in cisplatin-treated RAW 264.7 macrophages. In addition, ALPS alleviated cell damage by suppressing the mitochondrial apoptotic pathways in cisplatin-treated bone marrow-derived macrophages. Together, these findings suggested that ALPS may alleviate the toxic side effects of chemotherapeutic agents and act as a potential candidate for use as an effective adjuvant therapy.

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Neuroprotective effect of Annona muricata-derived polysaccharides in neuronal HT22 cell damage induced by hydrogen peroxide

Cited by 18 publications

References 44 publications

The Neuroprotective and Anti-Inflammatory Effects of Annona Muricata (Graviola) on Radiation-Induced Rat Sciatic Nerve Injury.

The Neuroprotective and Anti-Inflammatory Effects of Annona Muricata (Graviola) on Radiation-Induced Rat Sciatic Nerve Injury.

The neuroprotective and anti-inflammatory effects of Annona muricata (Graviola) on radiation-induced rat sciatic nerve injury

Polysaccharides from Annona muricata leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction

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