Neuroprotective effect of ginkgolide K on glutamate-induced cytotoxicity in PC 12 cells via inhibition of ROS generation and Ca2+ influx

Ma, Shuwei; Liu, Hongxia; Jiao, Haoyan; Wang, Liyan; Chen, Lvyi; Liang, Jun; Zhao, Ming; Zhang, Xiantao

doi:10.1016/j.neuro.2011.11.003

Cited by 86 publications

(53 citation statements)

References 53 publications

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“…4c) in the glutamate group markedly increased compared to those in the control group. Our results are similar to what has been reported after glutamate-induced excitotoxicity (Ma et al 2012). Notably, the administration of KHG26693 significantly prevented the formation of MDA (Fig.…”

Section: Khg26693 Attenuated Ros Nox Activity and Lipid Peroxidatiosupporting

confidence: 92%

“…Antioxidant enzymes such as superoxide dismutase (SOD), glutathione reductase (GR), catalase, nuclear factor erythroid 2-related factor 2 (Nrf2), glutamate cysteine ligase catalytic subunit (GCLC), and heme oxygenase-1 (HO-1) act as a cellular defense mechanism against oxidative stress (Babu et al 2011;Zhang et al 2013). In addition, reduced glutathione (GSH) has an important role in the antioxidant defense of cells by acting as a substrate for various peroxidases or by directly detoxifying ROS (Conrad and Sato 2012;Ma et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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N-Adamantyl-4-Methylthiazol-2-Amine Attenuates Glutamate-Induced Oxidative Stress and Inflammation in the Brain

et al. 2017

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In this study, we explored the possible mechanisms underlying the neuroprotective and anti-oxidative effects of N-adamantyl-4-methylthiazol-2-amine (KHG26693) against in vivo glutamate-induced toxicity in the rat cerebral cortex. Our results showed that pretreatment with KHG26693 significantly attenuated glutamate-induced elevation of lipid peroxidation, tumor necrosis factor-α, interferon gamma, IFN-γ, interleukin-1β, nitric oxide, reactive oxygen species, NADPH oxidase, caspase-3, calpain activity, and Bax. Furthermore, KHG26693 pretreatment attenuated key antioxidant parameters such as levels of superoxide dismutase, catalase, glutathione, and glutathione reductase. KHG26693 also attenuated the protein levels of inducible nitric oxide synthase, neuronal nitric oxide synthase, nuclear factor erythroid 2-related factor 2, heme oxygenase-1, and glutamate cysteine ligase catalytic subunit caused by glutamate toxicity. Finally, KHG26693 mitigated glutamate-induced changes in mitochondrial ATP level and cytochrome oxidase c. Thus, KHG26693 functions as neuroprotective and anti-oxidative agent against glutamate-induced toxicity through its antioxidant and anti-inflammatory activities in rat brain at least in part.

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Section: Khg26693 Attenuated Ros Nox Activity and Lipid Peroxidatiosupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

N-Adamantyl-4-Methylthiazol-2-Amine Attenuates Glutamate-Induced Oxidative Stress and Inflammation in the Brain

et al. 2017

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“…PC12 cell line is derived from a pheochromocytoma of rat adrenal medulla and can be induced to stop dividing and terminally differentiate when treated with high concentrations of glutamate [5,8,11]. This feature makes PC12 cells to be a useful model system for neuronal damage studies.…”

Section: Communicating Authormentioning

confidence: 99%

Original article Protective effect of paeoniflorin against glutamate-induced neurotoxicity in PC12 cells via Bcl-2/Bax signal pathway

Sun

Wang²,

Wu³

et al. 2012

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A b s t r a c t Paeoniflorin (PF), a monoterpene glycoside isolated from the aqueous extract of Radix Paeoniae Alba, is widely used in Traditional Chinese Medicine (TCM) for the treatment of neurodegenerative disorders such as Alzheimer's disease (AD) and Parkinson's disease (PD). In this study, we investigated the protective mechanism of PF on glutamate-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells. PC12 cells were cultured in vitro, cell viability was assessed by MTT assay, cell apoptosis as well as mitochondrial membrane potential (MMP) were detected by flow cytometric analysis and the expression profiles of apoptosis-related proteins including Bcl-2 and Bax were investigated by western blot. The results showed that PF could protect PC12 cells against glutamate-induced injury in a concentration-dependent manner and the mechanism of neuroprotective effect of PF was closely associated with up-regulation of

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“…This cell model is also generally employed to study cellular glutamate toxicity [18]. PC12 cells are highly sensitive to glutamate or hypoxia or A-Beta induced injury, therefore, we assumed that it is appropriate for PC12 to examine whether AEURA offers protection against glutamate or hypoxia or A-Beta induced cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

“…As expected, the survival of PC12 cells decreased with the increasing of concentrations of glutamate. We chose the optimal doses of 10 mM glutamate for cell viability test [18]. At 10 mM glutamate, about 55% of survival PC12 cells was observed from 76% at 10 μM glutamate to 18% at 150 mM glutamate.…”

Section: Glutamate Excitotoxicity Is Dose-dependent In Pc-12 Cell Culmentioning

confidence: 99%

Protective functions of AEURA in Cell Based Model of Stroke and Alzheimer disease

Jang-Yen¹

2017

J Neurosci Neurol Disord

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Stroke and neurodegenerative diseases including Alzheimer's disease (AD) are responsible for a major proportion of mortalities in the elderly. We have previously investigated novel mechanism-based therapies of AEURA in cell culture models against viral infection and in glutamate excitotoxity. In our new studies, we propose that the homeopathic formula AEURA could serve as a potential therapeutic agent for stroke & for AD. In examining AEURA treatment of PC12 cells exposed to glutamate excitotoxicity, hypoxia /re-oxygenation injury and A-Beta toxicity. We demonstrated an increased survival rate in AEURA treated cells by comparison to control cells. In examining the therapeutic potential of AEURA in PC12 cells this homeopathic agent was found to be neuroprotective against either glutamate induced toxicity, hypoxia /re-oxygenation stress or cell stress resulting from viral infection (with either HSV-1 or rhinovirus). Our ongoing studies involve examining the neuroprotective potential AEURA in vivo using rodent models of stroke & AD.

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Neuroprotective effect of ginkgolide K on glutamate-induced cytotoxicity in PC 12 cells via inhibition of ROS generation and Ca2+ influx

Cited by 86 publications

References 53 publications

N-Adamantyl-4-Methylthiazol-2-Amine Attenuates Glutamate-Induced Oxidative Stress and Inflammation in the Brain

N-Adamantyl-4-Methylthiazol-2-Amine Attenuates Glutamate-Induced Oxidative Stress and Inflammation in the Brain

Original article Protective effect of paeoniflorin against glutamate-induced neurotoxicity in PC12 cells via Bcl-2/Bax signal pathway

Protective functions of AEURA in Cell Based Model of Stroke and Alzheimer disease

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