Neuroprotective Effect of ent-Kaur-15-en-17-al-18-oic Acid on Amyloid Beta Peptide-Induced Oxidative Apoptosis in Alzheimer’s Disease

Zhang, Caiyun; Zhao, Xing‐Ming; Lin, Shiqi; Liu, Fangyuan; Ma, Jiahui; Han, Zhuo; Jia, Fu-Juan; Xie, Wenrui; Zhang, Qian; Li, Xia

doi:10.3390/molecules25010142

Cited by 14 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, their anti-amyloid capacity is similar to extracts from other medicinal plants that are considered neuroprotective, as well [ 74 , 75 , 76 ]. However, SFDE and SFEA do not completely restore the viability of the cells, as other individual compounds or specific extracts from other plants do [ 77 , 78 ].…”

Section: Discussionmentioning

confidence: 99%

Chemical Profiling and Antioxidant and Anti-Amyloid Capacities of Salvia fruticosa Extracts from Greece

Ververis,

Kyriakou,

Ioannou

et al. 2023

Plants

View full text Add to dashboard Cite

An increasingly common ailment in elderly persons is Alzheimer’s disease (AD), a neurodegenerative illness. Present treatment is restricted to alleviating symptoms; hence, there is a requirement to develop an effective approach to AD treatment. Salvia fruticosa (SF) is a medicinal plant with a documented neuroprotective potential. To identify extracts of increased neuroprotectivity, we partitioned the methanolic extract of SF aerial parts from Greece into several fractions, by employing solvents of different polarities. The fractions were chemically identified and evaluated for their antioxidancy and anti-neurotoxic potential against amyloid beta peptides 25–35 (Aβ25–35). Carnosol and carnosic acid were among the prominent compounds, while all partitions showed significant antioxidant capacity, with the diethyl ether and ethyl acetate partitions being the most potent. These, along with the aqueous and the butanolic fractions, demonstrated statistically significant anti-neurotoxic potential. Thus, our findings further validate the neuroprotective potential of SF and support its ethnopharmacological usage as an antioxidant. The particular properties found define SF as a promising source for obtaining extracts or bioactive compounds, possibly beneficial for generating AD-related functional foods or medications. Finally, our results encourage plant extract partitioning for acquiring fractions of enhanced biological properties.

show abstract

Section: Discussionmentioning

confidence: 99%

Chemical Profiling and Antioxidant and Anti-Amyloid Capacities of Salvia fruticosa Extracts from Greece

Ververis,

Kyriakou,

Ioannou

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…The blue crystals were solubilized, and the colorimetric intensity was measured at 570 nm and 690 nm on a microplate reader (Thermo Scientific Multiskan Spectrum). The percentage of cell viability was calculated relative to the colorimetric intensity of control cells (Zhang et al, 2020). MTT is used to assess cell viability and proliferation.…”

Section: Methodsmentioning

confidence: 99%

Microglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration

Goksu,

Kocanci,

Akinci

et al. 2024

Eur J of Neuroscience

View full text Add to dashboard Cite

A common pathological hallmark of neurodegenerative disorders is neuronal cell death, accompanied by neuroinflammation and oxidative stress. The vasoactive intestinal peptide (VIP) is a pleiotropic peptide that combines neuroprotective and immunomodulatory actions. The gene therapy field shows long‐term promise for treating a wide range of neurodegenerative diseases (ND). In this study, we aimed to investigate the in vitro efficacy of transduction of microglia using lentiviral gene therapy vectors encoding VIP (LentiVIP). Additionally, we tested the protective effects of the secretome derived from LentiVIP‐infected “immortalized human” microglia HMC3 cells, and cells treated with Synthetic VIP (SynVIP), against toxin‐induced neurodegeneration. First, LentiVIP, which stably expresses VIP, was generated and purified. VIP secretion in microglial conditioned media (MG CM) for LentiVIP‐infected HMC3 microglia cells was confirmed. Microglia cells were activated with lipopolysaccharide, and groups were formed as follows: 1) Control, 2) SynVIP‐treated, or 3) LentiVIP‐transduced. These MG CM were applied on an in vitro neurodegenerative model formed by differentiated (d)‐SH‐SY5Y cells. Then, cell survival analysis and apoptotic nuclear staining, besides measurement of oxidative/inflammatory parameters in CM of cells were performed. Activated MG CM reduced survival rates of both control and toxin‐applied (d)‐SH‐SY5Y cells, whereas LentiVIP‐infected MG CM and SynVIP‐treated ones exhibited better survival rates. These findings were supported by apoptotic nuclear evaluations of (d)‐SH‐SY5Y cells, alongside oxidative/inflammatory parameters in their CM. LentiVIP seems worthy of further studies for the treatment of ND because of the potential of gene therapy to treat diseases effectively with a single injection.

show abstract

“…The blue crystals were solublized and the intensity was measured colorimetrically at 570 nm and 690 nm on a microplate reader (Thermo Scienti c Multiskan Spectrum). The percentage of cell viability was calculated relative to the colorimetric intensity of control cells [33].…”

Section: Cell Viability Assaymentioning

confidence: 99%

Cromolyn sodium and masitinib combination inhibits fibroblast-myofibroblast transition and exerts additive cell-protective and antioxidant effects in a bleomycin-induced in vitro fibrosis model

Erol

Dirol

2022

Preprint

View full text Add to dashboard Cite

Background: Several in vitro and in vivo studies suggest that tyrosine kinase inhibitors may be effective in the treatment of idiopathic pulmonary fibrosis. In our previous study, we reported that combined treatment with masitinib, a tyrosine kinase inhibitor used to treat mastocytoma, and cromolyn sodium, a mast cell stabilizer drug used in allergic diseases, exerts synergistic cell-protective and antioxidant effects on an in vitro neurodegenerative model. In this study, we aimed to investigate the anti-fibrotic, cell-protective, and antioxidant effects of this drug combination on a bleomycin-induced in vitro model of pulmonary fibrosis. Methods: HFF-1 fibroblast cells were cultured and were divided into groups according to bleomycin and hydrogen peroxide (H2O2) applications, followed by treatment with cromolyn sodium/masitinib. The cell viability rates and metabolic activity were assessed with an MTT assay. Cell groups were compared morphologically under an inverted microscope. Additionally, Hoechst staining was performed for apoptotic nuclear assessment. Triple-immunofluorescence labeling (alpha-smooth muscle actin, CD44, DAPI) was performed to observe fibroblast-myofibroblast transition. Total oxidant and antioxidant capacities were measured in conditioned media of cell groups.Results: Cromolyn sodium and masitinib mono treatments significantly increased the cell viability rate of fibroblasts which was decreased by bleomycin and H2O2. Combined treatments of cromolyn sodium and masitinib increased cell viability rates more than monotherapy. Combined cromolyn sodium and masitinib exerted a protective effect on bleomycin-induced changes by preserving cell morphology with fewer apoptotic cells and a lower rate of fibroblast to myofibroblast transition, and improved arrangement of fibroblasts. Masitinib, cromolyn sodium, and their combination reduced the mean number of apoptotic cells. Combined cromolyn sodium and masitinib decreased the expression of α-SMA that was induced by bleomycin and increased the total antioxidant capacity while significantly decreasing the total oxidant capacity level that was increased by bleomycin and H2O2.Conclusions: We observed the additive anti-fibrotic, cell-protective and antioxidant effects of masitinib and cromolyn sodium combination in an in vitro fibrosis model. New combination therapies may offer an innovative treatment approach for pulmonary fibrosis, with the advantages of lower doses of both drugs, fewer side effects, and different mechanisms of action at the same time.

show abstract

Neuroprotective Effect of ent-Kaur-15-en-17-al-18-oic Acid on Amyloid Beta Peptide-Induced Oxidative Apoptosis in Alzheimer’s Disease

Cited by 14 publications

References 41 publications

Chemical Profiling and Antioxidant and Anti-Amyloid Capacities of Salvia fruticosa Extracts from Greece

Chemical Profiling and Antioxidant and Anti-Amyloid Capacities of Salvia fruticosa Extracts from Greece

Microglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration

Cromolyn sodium and masitinib combination inhibits fibroblast-myofibroblast transition and exerts additive cell-protective and antioxidant effects in a bleomycin-induced in vitro fibrosis model

Contact Info

Product

Resources

About