Neuroprotective effect of Centella asiatica extract (CAE) on experimentally induced parkinsonism in aged Sprague-Dawley rats

Abstract

-Reactive oxygen species (ROS) play an important role in ageing and age-related neurodegenerative changes including Parkinson's disease (PD). PD is characterized by signs of major oxidative stress and mitochondrial damage in the pars compacta of the substantia nigra. Present study was designed to investigate whether the Centella asiatica extract (CAE) would prevent 1-methyl-4-phenyl-1,2,3,6 -tetrahydropyridine (MPTP) -induced neurotoxicity in aged Sprague-Dawley rats. Adult, male Sprague-dawley rats of 300-350 g were divided into control, C. asiatica alone, MPTP alone (20 mg/kg, for 21 days) and MPTP with C. asiatica (300 mg/kg for 21 days) groups. Effect of aqueous extract of C. asiatica on oxidative biomarker levels in corpus striatum and hippocampus homogenate was examined. MPTP-chal---asiatica was effective in protecting the brain against neurodegenerative disorders such as Parkinsonism.

Anti-Parkinsonian drug discovery from herbal medicines: What have we got from neurotoxic models?

“…MPTP is typically administered systemically in mice because it is able to cross the BBB (Cannon and Greenamyre, 2010). Although rats are resistant to MPTP for unknown reasons, they were also used in some studies (Yang et al, 2001;Fujikawa et al, 2005b;Haleagrahara and Ponnusamy, 2010). Rotenone, which produces systemic complex I inhibition, is often chronically systemic administered in rats (Fujikawa et al, 2005a;Sanchez-Reus et al, 2007).…”

Efficiency of an eastern traditional medicine- asiatic acid on traumatic spinal cord injury: an experimental study

“…It also has protective effects against neurotoxixity. These effects of AA have been shown in stroke and some degenerative neurodisorders such as Parkinsonism and Alzheimer Disease (7,10,12).…”

“…These are flavonoids, triterpenes and polyacetylenes. Some of the triterpenoids compounds are madecassoside, madecassic acid, asiaticoside, and AA (7,14).…”

Decreasing α-synuclein aggregation by methanolic extract of Centella asiatica in zebrafish Parkinson's model

“…Aqueous extract of C. asiatica on intra cerebrovascular streptozocin-induced memory associated with the sporadic type of Alzheimer's disease and pentylenetetrazoleinduced memory loss in rats showed the increasing neuroprotection through suppression of malondialdehyde and increasing antioxidant enzyme catalase, superoxide dismutase and glutathione [51,52] . Another experiment showed that C. asiatica protects rotenone-induced cells through the stabilization of mitochondrial membrane potential and inhibiting voltage-dependent anion channel enzyme [34,35]. It seems that antioxidant and anti-inflammatory property of C. asiatica could protect rotenone-induced zebrafish motility through the stabilization of dopamine synthesis, availability and its kinetics.…”

“…This condition may be caused by antioxidant and anti-inflammatory property of C. asiatica [33]. Haleagrahara and Ponnusamy [34] reported that supplementation of C. asiatica decreased lipid hydroperoxide, protein carbonyl content, and significantly increased total antioxidant and antioxidant enzyme level in rat induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Asiatic acid, one of the major components in C. asiatica could protect rotenone-induced SH-SY5Y cell through the stabilization of mitochondrial membrane potential, decreasing voltagedependent anion channel both in mRNA and protein level [35].…”