Neuroprotective effect of Centella asiatica leaf extract on hippocampal CA3 neurons: A quantitative study in mice

Hemamalini,; Rao, Muddanna S.

doi:10.1016/j.jasi.2016.08.134

Cited by 5 publications

(9 citation statements)

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“…Neurons are the basic functional and structural components of the nervous system. Several factors, such as stress, alter the structural makeup of neurons, in particular their dendritic arborization, synaptic junctions, neurochemical components, and functions ( Hemamalini, 2013 ). Chronic stress exposure dynamically alters the complexity of dendrites, specifically in the neurons of the PFC, amygdala, and hippocampus.…”

Section: Discussionmentioning

confidence: 99%

Ameliorating Effect of Morin Hydrate on Chronic Restraint Stress-induced Biochemical Disruption, Neuronal, and Behavioral Dysfunctions in BALB/c Mice

Akinluyi

Aderibigbe

Adeoluwa

et al. 2022

BCN

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Introduction: Morin hydrate (MH) is a bioflavonoid component of many fruits and vegetables. Our previous research demonstrated that MH provides neuroprotection in mouse models of acute restraint stress and sleep deprivation by attenuating hippocampal neuronal damage and enhancing memory. Based on these findings, our study investigated the role of MH in chronic stress-induced neuronal and biochemical perturbations in BALB/c mice. Methods: Male BALB/c mice were divided into 6 groups (n=6). Groups 1 and 2 received vehicle (10 mL/kg normal saline), groups 3-5 received MH (5, 10, 20 mg/kg IP), while group 6 received ginseng (25 mg/kg) daily and 30 minutes afterward were restrained in a plastic cylindrical restrainer for 14 days. Results: Immobility time in the forced swim test increased in the MH-treated group, indicating an antidepressant-like effect. Also, a reduction in frequency and duration of open arms exploration was observed in the elevated plus-maze (EPM) test in stressed mice, and administration of MH (5, 10, 20 mg/kg, IP) reversed these effects. An increase in blood levels of glucose, triglycerides, total cholesterol, and brain malondialdehyde and nitrite levels was observed in the stressed groups, which was reversed by MH. Furthermore, MH reversed the stress-induced reduction in HDL cholesterol and glutathione (GSH) levels and attenuated stress-induced alterations in the prefrontal cortex and hippocampus. Conclusion: Our findings suggest that MH attenuated chronic restraint stress-behavioral and biochemical perturbations, probably due to its capability to decrease oxidative stress and brain neuronal damage

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Section: Discussionmentioning

confidence: 99%

Ameliorating Effect of Morin Hydrate on Chronic Restraint Stress-induced Biochemical Disruption, Neuronal, and Behavioral Dysfunctions in BALB/c Mice

Akinluyi

Aderibigbe

Adeoluwa

et al. 2022

BCN

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“…They found that, at low concentrations, this extract can ensure neuron cells against oxidative pressure. This may point to the limit of this herb in restraining the caspase-9 pathway by blocking the creation of proapoptotic proteins, advancing antiapoptotic proteins and copying the last mentioned [43,44]. Despite the fact that this mechanism is evidently centered around neuroprotective impacts against apoptosis, such a system could be a subset in the recovery and upgrade of nerve cell wounds.…”

Section: Discussionmentioning

confidence: 99%

“…[43] But the cytoprotective property of Centella asiatica on the morphology of the hippocampal neuron has not been reported. [44] In this study numbers of mice were selected to receive high dose ketamine to induce anesthesia which is considered as stress, repeatedly for seven days in one group. In other group of mice received high dose ketamine with extract of Centella asiatica.…”

Section: Introductionmentioning

confidence: 99%

Histomorphological Effect of Centella Asiatica on Swiss Albino Mice Brain upon Ketamine Anesthetic Induced Memory Impairment

2020

ALST

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Introduction: Ketamine is a medication mainly used for starting and maintaining anesthesia. It induces a trancelike state while providing pain relief, sedation and memory loss. Ketamine causes morphological changes in the neuronal cells of growing rodent. Centella asiatica is used for memory enhancement in the ayurvadic system of medicine. It has been shown to be useful in improving learning and memory. Centella asiatica leaf extract treatment enhances neuronal dendritic arborization in hippocampus of growing rodent.Materials and Methods: Swiss albino mice were used for our experiment. Ketamine was administered subcutaneously and Centella-asiatica leaf extract was administered orally. Mice brain collected, grossed, processed and finally stained by Hematoxylin and Eosin (H&E) staining process for viewing cellular and tissue structure detail by pathologists. Results: Focal cellular damage was seen in CA2 and CA4 area of cornu ammonis (CA), expanded cytoplasmic eosinophilia saw in the pyramidal cell layers (PY) of CA2, expanded cytoplasmic eosinophilia found with obliteration of neurifibrilary components (NFE), typical pyramidal cell (NPY) in the CA4, ordinary engineering of dentate granule layers (DG) and hardly any cells were found with diminished size in regards to granule layer cells (GC) in the brain hippocampus of the high ketamine prompted mice. In the mouse brain hippocampus for high ketamine-Centella asiatica summed up cell destruction found in the CA1 to CA4 of CA. Expanded cytoplasmic eosinophilia found in the pyramidal cell layers of CA2 than high dose ketamine group. The vast majority of the pyramidal cells were necrotic (PYN) with annihilation of neurofibrilary components (NFE) in the pyramidal cell layers of CA4.

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“…may alter glucocorticoid activities and the adaptation response to stress. [38][39][40][41] Although a speculation, the reciprocal relationship between the cerebellum and HPA-axis was recently established by the discovery of dense glucocorticoids binding sites on the vermis. 42 In this study, we added biochemical evidence that C. asiatica (L.) Urb.…”

Section: Discussionmentioning

confidence: 99%

Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress

Sari¹,

Juananda²,

Ar-Rochmah³

et al. 2018

JmedScie

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Chronic stress produces glucocorticoid-induced neurotoxicity that may lead to alterations of the brain-derived neurotrophic factor (BDNF) concentration in the brain. Cerebellum is known to be severely affected by glucocorticoids-associated oxidative damage. Centella asiatica (L.) Urb. may protect neurons from oxidative damage. This study aimed to investigate the effect of ethanolic extract of C. asiatica (L.) Urb. leaf on the rat cerebellar BDNF levels following stress. Twenty young-adult male Sprague Dawley rats were randomly assigned into four experimental groups. The stress control group received aquadest, and the other groups were treated with different doses of the C. asiatica (L.) Urb. extract i.e 150 (CeA150), 300 (CeA300) and 600 (CeA600) mg/kg body weight/ day orally, respectively and followed by chronic footshock stress for 28 days. Upon completion of the experimental period, all animals were sacrificed and the cerebellar was isolated. The BDNF levels from the cerebellar tissue lysate was measured using ELISA. The mean BDNF levels of the cerebellar tissue in the stress control, CeA150, CeA300 and CeA600 groups were 1217.10±301.40; 771.46±241.45; 757.05±268.29; and 627.00±246.02 pg/mL, respectively. Post-hoc analysis showed a significant difference between the control and treatment groups (p< 0.05). In conclusion, the ethanolic extracts of the C. asiatica (L.) Urb. leaf decrease the cerebellar BDNF levels in rats after chronic stress.

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Neuroprotective effect of Centella asiatica leaf extract on hippocampal CA3 neurons: A quantitative study in mice

Cited by 5 publications

References 0 publications

Ameliorating Effect of Morin Hydrate on Chronic Restraint Stress-induced Biochemical Disruption, Neuronal, and Behavioral Dysfunctions in BALB/c Mice

Ameliorating Effect of Morin Hydrate on Chronic Restraint Stress-induced Biochemical Disruption, Neuronal, and Behavioral Dysfunctions in BALB/c Mice

Histomorphological Effect of Centella Asiatica on Swiss Albino Mice Brain upon Ketamine Anesthetic Induced Memory Impairment

Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress

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