Neuroprotective Assessment of Moringa oleifera Leaves Extract against Oxidative-Stress-Induced Cytotoxicity in SHSY5Y Neuroblastoma Cells

Hashim, Farah J.; Vichitphan, Sukanda; Boonsiri, Patcharee; Vichitphan, Kanit

doi:10.3390/plants10050889

Cited by 21 publications

(11 citation statements)

References 68 publications

(42 reference statements)

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“…Oxidative stress is a major cause of neurodegenerative disease; thus, this inhibition is considered a promising strategy to prevent neurodegenerative disease. Ample evidence showed the potent antioxidant effect of M. oleifera in most of the parts of this plant ( 45 ), as previously described also elsewhere in human neuroblastoma (SH-SY5Y cell) ( 46 ). In this study, the TAC level in serum, which represented oxidative status in the brain, was analyzed; and found that both MOO and MOE failed to ameliorate the increased TAC serum levels in mice treated with Sco.…”

Section: Discussionsupporting

confidence: 67%

Effects of Moringa oleifera in Two Independents Formulation and as Neuroprotective Agent Against Scopolamine-Induced Memory Impairment in Mice

et al. 2022

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BackgroundThe cognitive deficit has frequently been found in the elderly population. Several studies have shown that every single part of Moringa oleifera, including leaves, roots, and seeds, has abundant micronutrients, such as flavonoids, which improve the neurobehavioral capacity. However, herb parts that display optimal neuropharmacological properties remain unknown.ObjectiveWe investigate whether M. oleifera seed oil (MOO) or aqueous M. oleifera leaves extracts (MOEs) may ameliorate memory impairment in mice induced with scopolamine (Sco). Additionally, the phytochemical analyses of those two independent formulations were analyzed.MethodsIn this study, 2 ml/kg body weight (BW) of MOO and 500 mg/kg BW of MOE were orally administered to the mice for 28 days, followed by intraperitoneal injection of Sco (1 mg/kg) at the day 22–28 to induce cognitive impairment in those mice.ResultsThe Sco group showed memory retention impairment represented by the Y-maze and novel object recognition tests, significant enhancement of acetylcholine esterase (AChE) activity in hippocampus tissue (p < 0.0001), and increased the level of total antioxidant capacity (TAOC) in serum. Interestingly, the Sco-induced memory defect was improved and completely blunted the AChE exacerbation in Sco+MOO-treated mice (p < 0.0001), although the TAOC level was comparable among the groups. Mechanistically, both tropomyosin receptor kinase B (TrkB), as a brain-derived neurotrophic factor-receptor, and nuclear factor-kappa-light-chain-enhancer of activated B cells (NF-κB) protein expressions were enhanced with the hippocampus isolated from the Sco group. Nonetheless, pretreatment with MOO only, but not with MOE, ameliorated the enhanced protein expression levels of TrkB and NF-κB (p < 0.05 and p = 0.09, respectively).ConclusionOur data reveal that MOO is preferable to MOE as a neuroprotective as evidenced by improving memory impairment. This effect, at least in part, through inhibiting the AChE and NF-κB activities and modulating the TrkB expression level.

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Section: Discussionsupporting

confidence: 67%

Effects of Moringa oleifera in Two Independents Formulation and as Neuroprotective Agent Against Scopolamine-Induced Memory Impairment in Mice

et al. 2022

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“…This study has observed the neuroprotective effect of the seed extracts in both stages of ischemic stages by the increase in animal survival rate, improved cognitive impairment, enhanced neuroplasticity, hippocampal neurogenesis, and cholinergic systems as also supported by a previous finding (Zhou et al, 2018). Hashim et al (2021) demonstrated that the ethanolic extracts of Alpinia galanga rhizomes (ARE), Panax ginseng leaves (PLE), Alpinia galanga leaves (ALE), M. oleifera leaves (MLE), Vitis vinifera seeds (VSE), and Panax ginseng rhizomes (PRE) were determined for their neuroprotective potential on human neuroblastoma (SHSY5Y) cells. MLE has an overall high reading of DPPH, FRAP, ROS scavenging, and nitro-blue tetrazolium (NBT) test via 2,7-dichlorodihydrofluorescein diacetate (DCFHDA) assay.…”

Section: Oleifera Extractssupporting

confidence: 84%

“… Hashim et al (2021) demonstrated that the ethanolic extracts of Alpinia galang a rhizomes (ARE), Panax ginseng leaves (PLE), Alpinia galanga leaves (ALE), M. oleifera leaves (MLE), Vitis vinifera seeds (VSE), and Panax ginseng rhizomes (PRE) were determined for their neuroprotective potential on human neuroblastoma (SHSY5Y) cells. MLE has an overall high reading of DPPH, FRAP, ROS scavenging, and nitro-blue tetrazolium (NBT) test via 2,7-dichlorodihydrofluorescein diacetate (DCFHDA) assay.…”

Section: Neuroprotective and Anti-neuroinflammatory Effects Of ...mentioning

confidence: 99%

An insight into the neuroprotective and anti-neuroinflammatory effects and mechanisms of Moringa oleifera

et al. 2023

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Neurodegenerative diseases (NDs) are sporadic maladies that affect patients’ lives with progressive neurological disabilities and reduced quality of life. Neuroinflammation and oxidative reaction are among the pivotal factors for neurodegenerative conditions, contributing to the progression of NDs, such as Parkinson’s disease (PD), Alzheimer’s disease (AD), multiple sclerosis (MS) and Huntington’s disease (HD). Management of NDs is still less than optimum due to its wide range of causative factors and influences, such as lifestyle, genetic variants, and environmental aspects. The neuroprotective and anti-neuroinflammatory activities of Moringa oleifera have been documented in numerous studies due to its richness of phytochemicals with antioxidant and anti-inflammatory properties. This review highlights up-to-date research findings on the anti-neuroinflammatory and neuroprotective effects of M. oleifera, including mechanisms against NDs. The information was gathered from databases, which include Scopus, Science Direct, Ovid-MEDLINE, Springer, and Elsevier. Neuroprotective effects of M. oleifera were mainly assessed by using the crude extracts in vitro and in vivo experiments. Isolated compounds from M. oleifera such as moringin, astragalin, and isoquercitrin, and identified compounds of M. oleifera such as phenolic acids and flavonoids (chlorogenic acid, gallic acid, ferulic acid, caffeic acid, kaempferol, quercetin, myricetin, (-)-epicatechin, and isoquercitrin) have been reported to have neuropharmacological activities. Therefore, these compounds may potentially contribute to the neuroprotective and anti-neuroinflammatory effects. More in-depth studies using in vivo animal models of neurological-related disorders and extensive preclinical investigations, such as pharmacokinetics, toxicity, and bioavailability studies are necessary before clinical trials can be carried out to develop M. oleifera constituents into neuroprotective agents.

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“…In addition to interacting with proteins, polyphenols also have other activities, namely as antioxidants that function to ward off free radicals. Polyphenol compounds may be produced by the ethanol extract of A. galanga [19]. The ethanolic extract of A. galanga had a very high IC50 value against NIH-3T3 fibroblast cells.…”

Section: Resultsmentioning

confidence: 99%

Systematic Review: Anticancer Potential of Active Compounds from Galangal (Alpinia galanga)

Suciati¹,

Maryati²

2022

Atlantis Highlights in Chemistry and Pharmaceutical Sciences

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Galangal (Alpinia galanga) is a plant that is widely spread in Southeast Asia and is widely used for cooking spices. Research related to the effect of A. galanga on cancer cells has been carried out. The purpose of this systematic review is to examine the potential of A. galanga as an anticancer agent and co-chemotherapy. The literature search was carried out through the Pubmed, Science Direct, Springer Link, DOAJ, and Garuda Portal databases. The keywords used were "Alpinia Galanga and Anticancer", "Alpinia Galanga and Cytotoxicity", "Lengkuas dan Sitotoksik", "Lengkuas dan Kanker", and "Alpinia Galanga and Cancer". Of the 965 articles found at the beginning of the search, 31 articles were obtained which had been filtered and discussed further. The results showed that the ethanolic extract of A. galanga had a cytotoxic effect because it contained antioxidant polyphenols that inhibited cell proliferation by stopping the cell cycle. The most abundant compounds in A. galanga as anticancer agents are 1'-Acetoxychavicol Acetate (ACA) and Galangin. ACA works to inhibit cancer cells by inducing apoptosis through mitochondrial depolarization and DNA fragmentation in the cell cycle phase. Meanwhile, Galangin works as a chemopreventive agent that reduces oxidative stress and inflammation by suppressing the MAPKs pathway.

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Neuroprotective Assessment of Moringa oleifera Leaves Extract against Oxidative-Stress-Induced Cytotoxicity in SHSY5Y Neuroblastoma Cells

Cited by 21 publications

References 68 publications

Effects of Moringa oleifera in Two Independents Formulation and as Neuroprotective Agent Against Scopolamine-Induced Memory Impairment in Mice

Effects of Moringa oleifera in Two Independents Formulation and as Neuroprotective Agent Against Scopolamine-Induced Memory Impairment in Mice

An insight into the neuroprotective and anti-neuroinflammatory effects and mechanisms of Moringa oleifera

Systematic Review: Anticancer Potential of Active Compounds from Galangal (Alpinia galanga)

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