Neuroprotective activity of natural products isolated from Senecio graciliflorus DC against corticosterone-induced impairment in SH-SY5Y cells

Abstract

Senecio gracili orus DC root extract was studied for secondary metabolite composition following bioactivity guided isolation technique. The ethyl acetate extract of Senecio gracili orus root yielded nine chemical constituents: 3,4-di-tert-butyl toluene, stigmasterol, β-sitosterol, 2β-(angeloyloxy)furanoeremophilane, gallic acid, 2β-{[(Z)-2-hydroxymethylbut-2enoyl]oxy}furanoeremophilane,1-Hydroxypentan-2-yl-4-methylbenzoate, sarcinnic acid, and sitosterol 3-Oβ-D-glucopyranoside. The structures of the chemical constituents were elucidated on the basis of spectral data analysis in the light of literature. All the compounds are being reported for the rst time from this plant. The isolated constituents were screened for in-vitro neuroprotective effects against corticostereone induced impairment. Among various isolated compounds, three natural products (sarcinnic acid, gallic acid and β-sitosterol) displayed robust neurotropic activity. These three compounds increased neuronal cell survival in differentiated neuroblastoma cells from high dose corticosterone (400µM) induced cell death. The studies are aimed to explore small molecules for treating neurodegeneration underlying various neurological disorders to restore neuronal cell plasticity. DeclarationsAcknowledgement: The authors (Showkat Ahmad Bhat and Loveleena Kaur) are thankful to CSIR and DST respectively for providing fellowship support.Ethical approval: The work was reviewed and approved by institutional ethics and publication committee. The committee has assigned CSIR-IIIM/IPR/00291 as the research paper number.

Anticancer activity and other biomedical properties of β‐sitosterol: Bridging phytochemistry and current pharmacological evidence for future translational approaches

“…β‐sitosterol isolated form Cassia timorensis demonstrated moderate activity against AChE with 49.1% inhibition (Azman et al, 2020). The compound has also showed neuroprotective effect as evident by enhanced cell viability in corticosterone‐induced toxicity in SHS5YS cells via AKT/ERK pathway (Jameel et al, 2021).…”