Neuroprotective actions of estradiol revisited

Azcoitia, Íñigo; Arévalo, María Ángeles; Nicola, Alejandro F. De; Garcia-Segura, Luis Miguel

doi:10.1016/j.tem.2011.08.002

Cited by 115 publications

(90 citation statements)

References 73 publications

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“…Accordingly, "24 h" incubation with the respective compounds yielded different results being either inhibitory on STS activity (E2, T, Org4094) or stimulating (iCR, E2), respectively. In brain tissue a positive increase in STS activity would be expected to be more beneficial promoting the reported neuro-protective and -reparative effects [1].…”

Section: Discussionmentioning

confidence: 99%

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Effects of black cohosh on estrogen biosynthesis in hippocampus of non-human primates ex vivo in vitro and in human neuroblastoma cells in vitro

et al. 2016

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Background: Estrogens have neuroprotective properties. The aim of the study was to assess the impact of an isopropanolic extract of Cimicifuga racemosa (iCR), 17ß-estradiol (E2), testosterone (T) and Tibolone's 3α-OH metabolite (Org4094) on local estrogen formation in hippocampus tissue from non-human primates ex vivo in vitro and human neuroblastoma cells (SH-SY5Y) in vitro. Methods: SH-SY5Y cells were incubated in RPMI 1640 medium containing 5 % steroid-depleted fetal calf serum for 3 days, and subsequently incubated in absence or presence of iCR at 10 μg/ml (n = 5) and 1 μg/ml (n = 5), E2 at 10 −8 M (n = 5), and 10 −6 M (n = 5), or T at 10 −8 M (n = 5), and 10 −6 M (n = 5), respectively, at 37°C for either 24 h or directly in cell extracts. Hippocampus tissue from healthy female cynomolgus macaques (n = 14) was homogenized and treated with iCR, E2 and Org4049 accordingly. STS activity was evaluated by incubating homogenized brain cells and tissue with [3H]-estrone sulfate and separating the products estrone (E1) and E2 by thin layer chromatography. STS activity was expressed as total estrogen formation (E1 + E2) fmol/mg of protein/min. Statistical comparisons were made using unpaired T-Test for comparing two sets of data and ANOVA to compare many groups at once. A p value < 0.05 was considered to be significant different. Results: Basal total estrogen formation was significantly higher in proliferative SH-SY5Y cells (1350.06 ± 109 E1 + E2 fmol/mg of protein/min) compared to hippocampus tissue (37.83 ± 3.9 E1 + E2 fmol/mg of protein/min; p < 0.01). ICR exerted a bidirectional action with an immediate strong inhibitory effect being followed by a stimulation of STS activity (85 % increase). E2 had a bidirectional dose-dependent long-term effect on STS activity (−23 % reduction and 18 % increase). T and Org4094 had an immediate strong inhibitory effect on STS activity that further maintained with 24 h T treatment (30 % decrease). Conclusions: Local estrogen formation within brain cells and tissue may be modulated by black cohosh, 17ß-estradiol, testosterone, and Tibolone's 3α-OH metabolite, respectively. The clinical implication of this finding has to be elucidated.

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Section: Discussionmentioning

confidence: 99%

“…promoting neuronal survival, neuritogenesis and synaptic function, reducing local inflammation of astrocytes and microglia, and facilitating remyelination, respectively [1]. Specifically, estrogens seem to display these characteristics when initiated during the critical window of opportunity close to menopause [2].…”

Section: Introductionmentioning

confidence: 99%

Effects of black cohosh on estrogen biosynthesis in hippocampus of non-human primates ex vivo in vitro and in human neuroblastoma cells in vitro

et al. 2016

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“…protective and/or regenerative benefits for vascular, neural, muscular and hepatic tissues following damage (Azcoitia et al 2011, Manson et al2007, Sipilä et al 2015, Hsu et al 2015. When circulating estrogen levels are diminished, as seen in post-menopausal females, many of the beneficial effects of estrogen on these tissues are also lost.…”

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confidence: 99%

“…Several studies have highlighted that a delay in estrogen replacement following ovariectomy will result in a loss of estrogen efficacy in restoring its regenerative and protective potential in neural and vascular tissues (Lenfant et al 2011, Azcoitia et al 2011, Suzuki et al 2007. It has been suggested that some of the controversies regarding the potential adverse or beneficial effects of estrogen and hormone replacement in the health of post-menopausal women and specifically in muscle tissue may be related to the length of time that passed before post-menopause hormone replacement was initiated in long term studies examining health effects of hormone replacement (Schierbeck et al 2012, Tiidus et al 2013, Gurney et al 2014).…”

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confidence: 99%

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Delay in post-ovariectomy estrogen replacement negates estrogen-induced augmentation of post-exercise muscle satellite cell proliferation

Mangan

Iqbal

Hubbard

et al. 2015

Can. J. Physiol. Pharmacol.

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https://mc06.manuscriptcentral.com/cjpp-pubs Aim: This study examined the effects of a delay in post-ovariectomy replacement of 17β-estradiol (estrogen) on the post-exercise proliferation of muscle satellite cells.Methods: Nine-week old, ovariectomized female Sprague-Dawley rats (n=64) were divided into eight groups based on: estrogen status (0.25 mg estrogen pellet or sham), exercise status (90 min run @ 17 m/min, -13.5° or unexercised) and estrogen replacement ("proximal"; estrogen replacement within twoweeks or "delayed"; estrogen replacement at eleven-weeks following ovariectomy).Results: Significant increases in satellite cells were found in the soleus and white gastrocnemius muscle (immunofluorescent co-localization of nuclei with Pax7) 72 hrs following eccentric exercise (p < 0.05) in all exercised groups. "Proximal" E2 replacement resulted in a further augmentation of muscle satellite cells in exercised rats (p < 0.05) relative to the delayed estrogen replacement group. Expression of PI3Kwas unaltered and phosphorylation of Akt relative to total Akt increased following estrogen supplementation and exercise. Exercise alone did not alter the expression levels of Akt.Conclusion: An 11 week delay in post-ovariectomy estrogen replacement negated the augmenting influence seen with "proximal" (2 week delay) post-ovariectomy estrogen replacement on post-exercise muscle satellite cell proliferation. This effect appears to be independent of the PI3K/Akt signaling pathway.

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Estrogenic Regulation of Neuroprotective and Neuroinflammatory Mechanisms: Implications for Depression and Cognition

et al. 2019

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Neuroprotective actions of estradiol revisited

Cited by 115 publications

References 73 publications

Effects of black cohosh on estrogen biosynthesis in hippocampus of non-human primates ex vivo in vitro and in human neuroblastoma cells in vitro

Effects of black cohosh on estrogen biosynthesis in hippocampus of non-human primates ex vivo in vitro and in human neuroblastoma cells in vitro

Delay in post-ovariectomy estrogen replacement negates estrogen-induced augmentation of post-exercise muscle satellite cell proliferation

Estrogenic Regulation of Neuroprotective and Neuroinflammatory Mechanisms: Implications for Depression and Cognition

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