Neuroprotection signaling pathway of nerve growth factor and brain-derived neurotrophic factor against staurosporine induced apoptosis in hippocampal H19-7 cells

Nguyen, Truong L.X.; Kim, Chung Kwon; Cho, Jun-Hee; Lee, Kyung-Hoon; Ahn, Jee-Yin

doi:10.3858/emm.2010.42.8.060

Cited by 81 publications

(65 citation statements)

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“…(Nitta et al 1999). Also in another study, BDNF and NGF were found to be neuroprotective in many stress models like Staurosporine (STS) and Etoposide (ETS) induced apoptotic cell death as demonstrated by inhibiting Trk receptor with K252a (Nguyen et al 2010). K252a is a well known Trk inhibitor which inhibits BDNF and NGF receptors i.e., TrkA and TrkB, respectively, at different concentrations.…”

Section: Discussionmentioning

confidence: 94%

Enriched Environment Prevents Hypobaric Hypoxia Induced Neurodegeneration and is Independent of Antioxidant Signaling

et al. 2012

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Hypobaric hypoxia (HH) induced neurodegeneration has been attributed to several factors including increased oxidative stress, glutamate excitotoxicity, decreased growth factors, apoptosis, etc. Though enriched environment (EE) has been known to have beneficial effects in various neurological disorders, its effect on HH mediated neurodegeneration remains to be studied. Therefore, the present study was conducted to explore the effect of EE on HH induced neurodegeneration. Male Sprague-Dawley rats were placed in enriched and standard conditions during exposure to HH (7 days) equivalent to an altitude of 25,000 ft. The effect of EE on oxidative stress markers, apoptosis, and corticosterone level in hippocampus was investigated. EE during exposure to HH was found to decrease neurodegeneration as evident from decreased caspase 3 expression and LDH leakage. However, no significant changes were observed in ROS, MDA, and antioxidant status of hippocampus. HH elevates corticosterone level and affected the diurnal corticoid rhythm which may contribute to neurodegeneration, whereas EE ameliorate this effect. Because of the association of neurotrophins and stress and/or corticosterone the BDNF and NGF levels were also examined and it was found that HH decreases their level but concurrent exposure to EE maintains their level. Moreover, inhibition of Tyrosine kinase receptor (Trk) with K252a nullifies the protective effect of EE, whereas Trk activation with agonist, amitriptyline showed protective effect similar to EE. Taken together, we conclude that EE has a potential to ameliorate HH mediated neuronal degeneration which may act through antioxidant independent pathway by modulation of neurotrophins.

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Section: Discussionmentioning

confidence: 94%

Enriched Environment Prevents Hypobaric Hypoxia Induced Neurodegeneration and is Independent of Antioxidant Signaling

et al. 2012

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“…BDNF has been reported to prevent glutamate-induced apoptosis in vitro (Almeida et al, 2005) and in vivo (Husson et al, 2005). In a similar way, Nguyen et al (2010) showed that NGF prevented staurosporine induced apoptosis on neuronal cell lines. The decrease in the levels of BDNF and NGF following 6 hours of isoflurane exposure indicates the neurotoxic effects of isoflurane.…”

Section: Discussionmentioning

confidence: 72%

Effects of vitamin C on inhalation anesthetic isoflurane-induced developmental, neuronal apoptosis in neonatal rats

Shuai

Wang

Xiong

et al. 2014

Bangladesh J Pharmacol

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Research ArticleEffects of vitamin C on inhalation anesthetic isoflurane-induced developmental, neuronal apoptosis in neonatal rats BJP IntroductionAbout 200 million patients worldwide are exposed to anesthesia and undergo surgery each year (Weiser et al., 2008;Moonesinghe et al., 2011). Volatile anesthetics such as isoflurane and sevoflurane are used in millions of young children each year during surgical procedures and imaging studies (Istaphanous and Loepke, 2009). Exposure to anesthetic cocktails (Yon et al., 2005;Lu et al., 2006;Sanders et al., 2009) or volatile agents such as isoflurane even as a single anesthetic (Yon et al., 2005;Ma et al., 2007) has been reported to be associated with widespread apoptotic neurodegeneration in the developing brains and contributes to the post-operative cognitive dysfunction (POCD) (Jevtovic-Todorovic et al., 2003;Satomoto et al., 2009;Brambrink et al., 2010;Kong et al., 2011;Paule et al., 2011; Li et al., 2013a,b).In rodent models, the effects of anesthesia have been evaluated following exposure during a time of peak brain development and synaptogenesis, typically at postnatal day 7 (P7) (Jevtovic-Todorovic et al., 2003;Stratmann et al., 2009b;Shih et al., 2012). Apoptosis has been observed to occur acutely in the period immediately following anesthesia (Jevtovic-Todorovic et al., 2003;Istaphanous et al., 2011;Shih et al., 2012), and the thalamus and hippocampus are known areas susceptible to extensive neurodegeneration (JevtovicTodorovic et al., 2003;Satomoto et al., 2009;Shih et al., 2012). AbstractDevelopmental abnormalities, neuronal apoptosis and associated cognitive impairment following isoflurane exposure in neonatal rodents have been reported. The study was undertaken to investigate the effect of vitamin C supplementation against isoflurane-induced neurotoxicity. Seven day old rats were exposed to 1.1% isoflurane, or air for 6 hours. Treatment groups were administered with vitamin C (30 mg/kg, orally) from postnatal day 1 (P1) to P10 and were exposed to isoflurane on P7. Isoflurane exposure induced apoptosis was determined by Fluoro-Jade C and terminal deoxynucleotidyltransferase-mediated 2'-deoxyuridine 5'-triphosphate nick-end labeling assay. Vitamin C considerably improved memory and learning impairments, modulated neuroapoptosis and improved expressions of brain-derived neurotrophic factor, nerve growth factor, Bcl-xL and decreased activated caspase-3 expressions. Thus, vitamin C effectively offered protection against isoflurane-induced neuronal apoptosis, learning and memory disturbances. Article Info

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“…12 Neutrophin, termasuk nerve growth factor (NGF) & brainderived neutrophic factor (BDNF) juga melindungi neuron dari proses eksitotoksisitas. 13 Rehabilitasi pasca stroke iskemik yang sudah ada saat ini memiliki 2 tujuan, yaitu meminimalkan cedera dan komplikasi yang terjadi. 14 Obat utama yang digunakan antara lain antiplatelet, antikoagulan, dan trombolitik.…”

Section: Pendahuluanunclassified

Ekstrak Kulit dan Biji Anggur (Vitis vinifera) Menurunkan Jumlah Sel Neuron yang Rusak, Volume Infark, dan Memperbaiki Fungsi Motorik pada Tikus Wistar Model Stroke Iskemik

Lukito¹,

Indra²

2016

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ABSTRAKStroke iskemik merupakan penyakit karena tersumbatnya pembuluh darah ke otak sehingga menyebabkan kerusakan neurologis. Kerusakan ini akibat perubahan seluler yang menyebabkan kerusakan neuron, edema otak, dan penurunan fungsi motorik. Ekstrak kulit dan biji anggur (Vitis vinifera) mengandung resveratrol yang mampu melintasi sawar darah otak dan memperbaiki kematian neuron melalui jalur patofisiologi seluler serta menginduksi ERK 1/2 pathway untuk regenerasi otak. Pada penelitian ini ingin diketahui korelasi antara perbaikan jumlah neuron yang rusak, volume infark, dan fungsi motorik setelah pemberian ekstrak kulit dan biji anggur pada tikus. Penelitian eksperimental ini menggunakan tikus (Rattus norvegicus) yang terbagi menjadi 5 kelompok yaitu (1) kontrol positif (+), (2) kontrol negatif (-), (3) dosis ekstrak 50 mg/kgBB, (4) dosis ekstrak 100 mg/kgBB, dan (5) dosis ekstrak 200 mg/kgBB. Ekstrak diberikan selama 2 minggu. Tikus diinduksi stroke dengan metode unilateral carotid artery occlusion selama 45 menit. Kemudian diuji fungsi motoriknya dengan ladder rung walking test. Setelah 2 minggu, dianalisis fungsi motorik, jumlah neuron yang rusak dan volume infark dengan metode hematoksilin & eosin. Berdasarkan uji Pearson terdapat korelasi yang cukup antara fungsi motorik dengan jumlah neuron yang rusak maupun volume infark (berturut-turut R = 0,306; R = 0,346). Sementara antara jumlah neuron yang rusak dengan volume infark terdapat korelasi yang kuat (R = 0,645, p = 0,002; p  0,05). Kesimpulannya terdapat korelasi antara perbaikan jumlah sel neuron yang rusak, volume infark, dan fungsi motorik.Kata kunci: Kulit dan biji anggur, Motorik, Infark, Regenerasi otak, Resveratrol, Stroke. Grape (Vitis vinifera) Peel and Seed Extract Decrease the Damaged Neurons, Infarc Volume, and Repair Motoric Function in Ischemic Stroke Rat Model ABSTRACTIschemic stroke is a disease that caused by blockage of blood circulation in brain, then resulting in neurological failure. This is due to cellular changes that lead to damaged neurons, brain edema, and motoric function impairment. Grape (Vitis vinifera) peel and seed extract contain resveratrol which is able to pass through blood brain barrier and improve neuron death through cellular pathway and induce ERK 1/2 pathway for brain regeneration. This study aim is to find the correlation between damaged neurons, infarct volume, and motoric function improvement after grape peel and seed extract treatment in rats. This experimental study used rats that were divided into 5 groups, (1) positive control, (2) negative control, (3) extract concentration 50 mg/kgBB, (4) extract concentration100mg/kgBW, and (5) extract concentration 200mg/kgBW. They were treated with grape peel and seed extract in 2 weeks. Inducing stroke performed with unilateral carotid artery occlusion for 45 minutes. Motoric function was assessed by ladder rung walking test. After 2 weeks, motoric function reassessment, damaged neurons and infarct volume were evaluated by hematoxylin & eosin staining. Based on Pears...

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Neuroprotection signaling pathway of nerve growth factor and brain-derived neurotrophic factor against staurosporine induced apoptosis in hippocampal H19-7 cells

Cited by 81 publications

References 56 publications

Enriched Environment Prevents Hypobaric Hypoxia Induced Neurodegeneration and is Independent of Antioxidant Signaling

Enriched Environment Prevents Hypobaric Hypoxia Induced Neurodegeneration and is Independent of Antioxidant Signaling

Effects of vitamin C on inhalation anesthetic isoflurane-induced developmental, neuronal apoptosis in neonatal rats

Ekstrak Kulit dan Biji Anggur (Vitis vinifera) Menurunkan Jumlah Sel Neuron yang Rusak, Volume Infark, dan Memperbaiki Fungsi Motorik pada Tikus Wistar Model Stroke Iskemik

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