Neuroprotection by the soy isoflavone, genistein, via inhibition of mitochondria-dependent apoptosis pathways and reactive oxygen induced-NF-κB activation in a cerebral ischemia mouse model

Qian, Yisong; Guan, Teng; Huang, Menghao; Cao, Liangxun; Li, Yunman; Cheng, Hao; Jin, Hangxia; Yu, Deyue

doi:10.1016/j.neuint.2012.03.011

Cited by 143 publications

(86 citation statements)

References 40 publications

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“…It is well known that genistein, an isofl avone found in soybeans and soy products, has both estrogenic (Cederroth et al, 2010) and antioxidant eff ects (Qian et al, 2012). In our study, the eff ect of genistein on the male off spring also appears to be negligible.…”

Section: Discussionsupporting

confidence: 39%

Disruption of reproductive development in male rat offspring following gestational and lactational exposure to di-(2-ethylhexyl) phthalate and genistein

et al. 2013

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Studies of developmental eff ects of mixtures of endocrine disrupters on the male reproductive system are of great concern. In this study, the reproductive eff ects of the co-administration of di-2-(ethylhexyl) phthalate (DEHP) and genistein (GEN) during pregnancy and lactation were studied in male rat off spring. Pregnant Sprague-Dawley rats were gavaged from gestation day 3 to postnatal day 21 with vehicle control, DEHP 250 mg⁄kg body weight (bw)⁄day, GEN 50 mg⁄kg bw⁄day, GEN 400 mg⁄kg bw⁄day, and two combinations of the two compounds (DEHP 250 mg⁄kg bw⁄day + GEN 50 mg⁄kg bw⁄day, DEHP 250 mg⁄kg bw⁄day + GEN 400 mg⁄kg bw⁄day). The outcomes studied were general morphometry (weight, AGD), testicular histology, testosterone levels, and expression at the mRNA level of genes involved in steroidogenesis. Organ coeffi cient, AGD / body weight 1 / 3 , serum testosterone concentration and genes involved in steroidogenic pathway expression when exposed to DEHP (250mg/kg bw⁄day), GEN(50mg/kg bw⁄day) or GEN(400mg/kg bw⁄day) alone were not signifi cantly diff erent from the control group. When exposed to (DEHP 250mg/kg bw⁄day +GEN 50mg/kg bw⁄day) together during pregnancy and lactation, serum testosterone concentration, epididymis coeffi cient and Cypal17a1,Scarb1 m RNA expression signifi cantly decreased compared to the control and GEN(50mg/kg bw⁄day). When exposed to (DEHP 250mg/kg bw⁄day +GEN 400mg/kg bw⁄day) together during pregnancy and lactation, AGD / body weight 1 / 3 , serum testosterone concentration, testis and epididymis coeffi cient and Star, Cypal17a1 mRNA expression appeared signifi cantly decreased compared to the control and DEHP/GEN single exposure, together with developmental impairment of seminiferous tubules and seminiferous epithelium. Overall, co-administration of DEHP and GEN during gestation and lactation seem to acts in a cumulative manner to induce the most signifi cant alterations in the neonate, especially with GEN at high dose, although the eff ect of the DEHP-GEN mixture on adult off spring should be observed further.

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Section: Discussionsupporting

confidence: 39%

Disruption of reproductive development in male rat offspring following gestational and lactational exposure to di-(2-ethylhexyl) phthalate and genistein

et al. 2013

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“…Stimulated by extracellular functions, κBα can be degraded, which results in the activation of p65. The activation of NF-κB induces the expression of certain genes, including inflammatory factors, proliferation or pro-apoptosis factors (22). In the present study, it was also found that sophoridine significantly decreases the protein expression of NF-κB in D283-Med cells.…”

Section: Discussionsupporting

confidence: 74%

Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1

Yue

Pan

et al. 2017

Oncol Lett

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Abstract. Sophoridine is an alkaloid extracted from Sophora alopecuroides that has extensive pharmacological actions. In the present study, the effect of sophoridine on cell growth of human medulloblastoma and its mechanism were investigated. Human medulloblastoma D283-Med cells were incubated with 0, 0.5, 1 or 2 mg/ml sophoridine for 24, 48 or 72 h. Cell proliferation and cytotoxicity were analyzed using MTT and lactate dehydrogenase assays, respectively. Next, analyses of cell apoptosis and caspase-3/8 activity were performed using flow cytometry or spectrophotometry, respectively. Lastly, the change in FoxM1, TrkB, BDNF, NF-κB and AP-1 expression was investigated using western blot analysis. In the present study, treatment with sophoridine significantly suppressed cell growth and induced apoptosis in human medulloblastoma cells. In addition, sophoridine significantly increased cytotoxicity and caspase-3/8 activity in human medulloblastoma. Finally, it was found that sophoridine suppresses the protein expression of FoxM1, TrkB, BDNF NF-κB and AP-1 in human medulloblastoma cells. The present study suggests that sophoridine suppresses cell growth of human medulloblastoma through the inhibition of the FoxM1, NF-κB and AP-1 signaling pathway.

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“…Which is the upstream mediator, ROS or NF-κB? ROS is well known to be involved in diverse physiological and pathological processes, and oxidative stress can activate the NF-κB pathway (Barchowsky et al, 1996;Qian et al, 2012). To explore whether or not ROS is the upstream mediator of NF-κB, ROS scavenger NAC was added to plumbagin treated H460 cells.…”

Section: Discussionmentioning

confidence: 99%

Plumbagin from Plumbago Zeylanica L Induces Apoptosis in Human Non-small Cell Lung Cancer Cell Lines through NF-κB Inactivation

Xu¹,

Shen²,

Liu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Neuroprotection by the soy isoflavone, genistein, via inhibition of mitochondria-dependent apoptosis pathways and reactive oxygen induced-NF-κB activation in a cerebral ischemia mouse model

Cited by 143 publications

References 40 publications

Disruption of reproductive development in male rat offspring following gestational and lactational exposure to di-(2-ethylhexyl) phthalate and genistein

Disruption of reproductive development in male rat offspring following gestational and lactational exposure to di-(2-ethylhexyl) phthalate and genistein

Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1

Plumbagin from Plumbago Zeylanica L Induces Apoptosis in Human Non-small Cell Lung Cancer Cell Lines through NF-κB Inactivation

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