Neuroprotection and neuronal dysfunction upon repetitive inhibition of oxidative phosphorylation

Hellweg, Rainer; Arnim, Christine A. F. Von; Büchner, Maren; Huber, Roman; Riepe, M. W.

doi:10.1016/s0014-4886(03)00127-4

Cited by 80 publications

(48 citation statements)

References 56 publications

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“…Using an improved fluorometric ELISA, BDNF protein content in brain tissue was quantitatively evaluated. As described in the methods section, the ELISA procedure employed here is characterized by a very high sensitivity and is far superior to alternative methods described for the quantification of the BDNF protein such as immunohistochemistry or immunoblotting [23,24]. We would also like to point out that earlier studies in experimental mice yielded very similar absolute BDNF concentrations to the values reported here.…”

Section: Discussionmentioning

confidence: 56%

Increased brain-derived neurotrophic factor (BDNF) protein concentrations in mice lacking brain serotonin

Kronenberg

Mosienko

Gertz

et al. 2015

Eur Arch Psychiatry Clin Neurosci

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The interplay between BDNF signaling and the serotonergic system remains incompletely understood. Using a highly sensitive enzyme-linked immunosorbent assay, we studied BDNF concentrations in hippocampus and cortex of two mouse models of altered serotonin signaling: tryptophan hydroxylase (Tph)2-deficient (Tph2 -/-) mice lacking brain serotonin and serotonin transporter (SERT) deficient (SERT -/-) mice lacking serotonin re-uptake. Surprisingly, hippocampal BDNF was significantly elevated in Tph2 -/-mice, whereas no significant changes were observed in SERT -/-mice. Furthermore, BDNF levels were increased in the prefrontal cortex of Tph2 -/-but not of SERT -/-mice. Our results emphasize the interaction between serotonin signaling and BDNF. Complete lack of brain serotonin induces BDNF expression.

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Section: Discussionmentioning

confidence: 56%

Increased brain-derived neurotrophic factor (BDNF) protein concentrations in mice lacking brain serotonin

Kronenberg

Mosienko

Gertz

et al. 2015

Eur Arch Psychiatry Clin Neurosci

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“…Endogenous levels of BDNF were measured in the rethawed homogenates using commercial ELISA kits in principle according to the manufacturer's instructions (Promega) but adapted to a fluorometric technique as described in detail previously (Hellweg et al, 2003). BDNF content was expressed as equivalents of recombinant human BDNF.…”

Section: Quantification Of Cerebral Neurotransmitter and Bdnf Levelsmentioning

confidence: 99%

“…Determinations of recovery and specific and unspecific neurotrophin binding (the latter against mouse IgG1 obtained from MOPC 21) involved quadruplicate fluorescence determinations for each tissue sample. Using this improved fluorometric ELISA, it was feasible to quantify BDNF in brain tissue with a minimal wet weight of ϳ5 pg (Hellweg et al, 2003(Hellweg et al, , 2006. BDNF levels were expressed as picograms per milligram of tissue (wet weight).…”

Section: Quantification Of Cerebral Neurotransmitter and Bdnf Levelsmentioning

confidence: 99%

Folate Deficiency Induces Neurodegeneration and Brain Dysfunction in Mice Lacking Uracil DNA Glycosylase

Kronenberg

Harms²,

Sobol³

et al. 2008

J. Neurosci.

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Folate deficiency and resultant increased homocysteine levels have been linked experimentally and epidemiologically with neurodegenerative conditions like stroke and dementia. Moreover, folate deficiency has been implicated in the pathogenesis of psychiatric disorders, most notably depression. We hypothesized that the pathogenic mechanisms include uracil misincorporation and, therefore, analyzed the effects of folate deficiency in mice lacking uracil DNA glycosylase (UngϪ/Ϫ) versus wild-type controls. Folate depletion increased nuclear mutation rates in UngϪ/Ϫ embryonic fibroblasts, and conferred death of cultured UngϪ/Ϫ hippocampal neurons. Feeding animals a folate-deficient diet (FD) for 3 months induced degeneration of CA3 pyramidal neurons in UngϪ/Ϫ but not Ung؉/؉ mice along with decreased hippocampal expression of brain-derived neurotrophic factor protein and decreased brain levels of antioxidant glutathione. Furthermore, FD induced cognitive deficits and mood alterations such as anxious and despair-like behaviors that were aggravated in UngϪ/Ϫ mice. Independent of Ung genotype, FD increased plasma homocysteine levels, altered brain monoamine metabolism, and inhibited adult hippocampal neurogenesis. These results indicate that impaired uracil repair is involved in neurodegeneration and neuropsychiatric dysfunction induced by experimental folate deficiency.

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“…Endogenous levels of BDNF were measured in the rethawed serum samples using commercial ELISA kits in principle according to the manufacturer's instructions (Promega Inc., Mannheim, Germany), but adapted to the fluorometric technique used also for nerve growth factor determination (Hellweg et al, 2003) and described in detail previously (Hellweg et al, 1989). The BDNF content was expressed as equivalents of recombinant human BDNF.…”

Section: Measurement Of Bdnf Levelsmentioning

confidence: 99%

“…The detection limit of the assay was 1 pg/ml. Determinations of recovery, specific and unspecific neurotrophin binding (the latter against mouse IgG 1 obtained from MOPC 21) involved quadruplicate fluorescence determinations for each serum sample (Hellweg et al, 2003).…”

Section: Measurement Of Bdnf Levelsmentioning

confidence: 99%

Association of BDNF Serum Concentrations with Central Serotonergic Activity: Evidence from Auditory Signal Processing

Lang

Hellweg

Gallinat

2005

Neuropsychopharmacol

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Disturbances of serotonergic neurotransmission in the brain have been implicated in the pathogenesis and maintenance of several psychiatric disorders. According to recent preclinical and clinical studies, the loudness dependence of auditory evoked potentials (LD) is related to the central serotonergic neurotransmission in humans. As the serotonergic phenotype has been reported to be associated with brain-derived neurotrophic factor (BDNF), we studied whether BDNF serum concentrations are related to LD in 109 healthy human volunteers (62 male, 47 female, age: 42.5713.1 years). Pearson correlation showed a significant negative correlation between the BDNF serum concentrations and the LD measured at Fz (r ¼ À0.259, p ¼ 0.007) and a trend for the Cz electrode (r ¼ À0.185, p ¼ 0.055). Although this association needs to be replicated, the results are in line with the assumption that low serum BDNF levels reflect low central serotonergic neurotransmission as indicated by a strong LD.

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Neuroprotection and neuronal dysfunction upon repetitive inhibition of oxidative phosphorylation

Cited by 80 publications

References 56 publications

Increased brain-derived neurotrophic factor (BDNF) protein concentrations in mice lacking brain serotonin

Increased brain-derived neurotrophic factor (BDNF) protein concentrations in mice lacking brain serotonin

Folate Deficiency Induces Neurodegeneration and Brain Dysfunction in Mice Lacking Uracil DNA Glycosylase

Association of BDNF Serum Concentrations with Central Serotonergic Activity: Evidence from Auditory Signal Processing

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