Neuroplasticity and Repair in Rodent Neurotoxic Models of Spinal Motoneuron Disease

Gulino, Rosario

doi:10.1155/2016/2769735

Cited by 5 publications

(3 citation statements)

References 164 publications

(230 reference statements)

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“…This is a simple and straightforward method to investigate the possible occurrence of compensatory changes in both the muscle and spinal cord. The results showed that, following the lesion, the skeletal muscle became atrophic and displayed electromyographic activity similar to that observed in ALS patients [ 96 , 97 , 98 , 99 , 100 ]. CTB-SAP is a prime example of how a single Saporin conjugate can be used in a variety of ways.…”

Section: Behavior Disease and Animal Modelssupporting

confidence: 67%

Saporin as a Commercial Reagent: Its Uses and Unexpected Impacts in the Biological Sciences—Tools from the Plant Kingdom

Ancheta¹,

Shramm²,

Bouajram³

et al. 2022

Toxins

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Saporin is a ribosome-inactivating protein that can cause inhibition of protein synthesis and causes cell death when delivered inside a cell. Development of commercial Saporin results in a technology termed ‘molecular surgery’, with Saporin as the scalpel. Its low toxicity (it has no efficient method of cell entry) and sturdy structure make Saporin a safe and simple molecule for many purposes. The most popular applications use experimental molecules that deliver Saporin via an add-on targeting molecule. These add-ons come in several forms: peptides, protein ligands, antibodies, even DNA fragments that mimic cell-binding ligands. Cells that do not express the targeted cell surface marker will not be affected. This review will highlight some newer efforts and discuss significant and unexpected impacts on science that molecular surgery has yielded over the last almost four decades. There are remarkable changes in fields such as the Neurosciences with models for Alzheimer’s Disease and epilepsy, and game-changing effects in the study of pain and itch. Many other uses are also discussed to record the wide-reaching impact of Saporin in research and drug development.

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Section: Behavior Disease and Animal Modelssupporting

confidence: 67%

Saporin as a Commercial Reagent: Its Uses and Unexpected Impacts in the Biological Sciences—Tools from the Plant Kingdom

Ancheta¹,

Shramm²,

Bouajram³

et al. 2022

Toxins

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show abstract

“…For instance, in young feline animals, this ability returns spontaneously, while regular training is required for adult cats to accelerate these mechanisms (de Leon et al, 1998). NP mechanisms have also been studied in neurotoxic rodent models of spinal MN disease (Gulino, 2016), where specific neurotoxins were used to induce selective depletion of spinal MN (cholera toxin B), finding plastic changes in the surviving motoneurons. Thus, the manipulation of these surviving MNs could potentially produce a functional restoration, stimulating these MN compensatory mechanisms (Gulino et al, 2019).…”

Section: Amyotrophic Lateral Sclerosismentioning

confidence: 99%

Molecular and microstructural biomarkers of neuroplasticity in neurodegenerative disorders through preclinical and diffusion magnetic resonance imaging studies

Gatto¹

2020

Journal of Integrative Neuroscience

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Advances in the understanding of genetic and molecular mechanisms and imaging technologies have opened a new window of research possibilities to address dynamic processes associated with neuroplasticity in physiologically intact models of neurodegenerative diseases. This review aims to: (i) establish the most relevant molecular mechanisms, as well as cellular and structural biomarkers in the study of neuroplasticity; (ii) introduce different neurodegenerative diseases in animal models that contribute to our knowledge of neuroplasticity; and (iii) illustrate the capabilities and limitations of current diffusion magnetic resonance imaging techniques to study cortical plasticity, as well as the use of alternative diffusion models.

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“…Previous studies in our laboratory have shown some mechanisms of SC plasticity in a mouse model of motoneuron depletion induced by intramuscular injection of the retrogradely transported, ribosome-inactivating toxin, cholera toxin-B saporin (CTB-Sap) [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Sonic Hedgehog and TDP-43 Participate in the Spontaneous Locomotor Recovery in a Mouse Model of Spinal Motoneuron Disease

Gulino

Parenti

Gulisano

2017

JFMK

Self Cite

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Several studies have attempted to repair the damaged spinal cord (SC) by stimulating neurogenesis or neuroplasticity, with limited success. Sonic hedgehog (Shh) is involved in neural induction and stem cell functioning, but recent findings also suggest its role in regeneration and functional recovery. Transactive response DNA-binding protein of 43 kDa (TDP-43) is a nuclear DNA/RNA binding protein involved in transcription and RNA processing. Recent findings have reported cytoplasmic inclusions containing TDP-43 in amyotrophic lateral sclerosis. Although substantial attention has been given to the toxic effects of this protein, the functional role of TDP-43 remains largely unclear. We used a mouse model of neurotoxic motoneuron depletion to study the role of the above-described factors in the compensatory changes occurring after the lesion. The injection of cholera toxin-B saporin into the gastrocnemius muscle caused a partial motoneuron death accompanied by an impairment of locomotion. Interestingly, motor activity was significantly restored as soon as one month later. Moreover, we observed an activity-dependent modification of Shh and synaptic proteins: synapsin-I and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors. Notably, the motor performance of lesioned animals correlated with the expression of synapsin-I and Shh. Conversely, the expression of Shh significantly correlated with the levels of synapsin-I, GluR2, and TDP-43. The results suggest that Shh and TDP-43 are crucial parts of a complex mechanism of neuroplasticity in a mouse model of SC motoneuron disease.

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Neuroplasticity and Repair in Rodent Neurotoxic Models of Spinal Motoneuron Disease

Cited by 5 publications

References 164 publications

Saporin as a Commercial Reagent: Its Uses and Unexpected Impacts in the Biological Sciences—Tools from the Plant Kingdom

Saporin as a Commercial Reagent: Its Uses and Unexpected Impacts in the Biological Sciences—Tools from the Plant Kingdom

Molecular and microstructural biomarkers of neuroplasticity in neurodegenerative disorders through preclinical and diffusion magnetic resonance imaging studies

Sonic Hedgehog and TDP-43 Participate in the Spontaneous Locomotor Recovery in a Mouse Model of Spinal Motoneuron Disease

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