Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer

Abstract

Background Neuropilin1 (NRP1) participates in cancer cell proliferation, migration, and metastasis as a multifunctional co‐receptor by interacting with multiple signal pathways, but few studies have addressed the precise function of NRP1 in pancreatic cancer (PACA) cells. We aimed to study whether NRP1 gene silencing involved in the proliferation and migration of PACA cells in vitro. Methods A lentiviral vector expressing NRP1 shRNA was constructed and transfected into human PACA cells (CFPAC‐1 and PANC‐1). The expression of NRP1 protein and mRNA was detected by Western blot and quantitative real‐time polymerase chain reaction (qRT‐PCR) assay, respectively. CCK‐8 assay, wound healing assay, and transwell assay were conducted to examine the effect of NRP1 silencing on cells proliferation and migration capability. Results Results of qRT‐PCR and Western blot showed successfully established, stably transfected shRNA‐NRP1 cells in PACA cells. The proliferation capacity of PACA cells in NRP1 shRNA group was lower significantly than that in the negative control (NC) group (P < .05). The invasion and migration capability of PACA cells in NRP1 shRNA group was lower significantly than that in the NC group (P < .01). Conclusions NRP1‐shRNA lentiviral interference vectors can effectively decrease NRP1 gene expression in PACA cells, thereby inhibiting cells proliferation and migration, which provides a basis for finding a valuable therapeutic target for PACA therapy.

NEUROD1 reinforces endocrine cell fate acquisition in pancreatic development

“…Notably, 26 genes were linked to programmed cell death , which highly overlapped with the cell adhesion cohort. Many of those genes were related to various kinds of neoplasia progression ( Tpx2 , Id3 , Top2a , Dab2 , Mecom , Nrp1 107 – 112 ), including pancreatic ductal adenocarcinoma, or pancreatitis ( Gsn 113 ). Furthermore, we observed an increased expression in genes involved in the MAPK/ERK cascade , which reportedly restrains endocrine specification 114 .…”

The Connection between MicroRNAs and Oral Cancer Pathogenesis: Emerging Biomarkers in Oral Cancer Management

“…Tumorigenesis of oral cancer is stimulated by the capability of miR-320 to target the 3′-UTR of Neuropilin 1 ( NRP1 ) [ 97 ]. NRP1 functions as a promoter of proliferation, survival, and migration/invasion of tumor cells and endothelial cells in various tumor type [ 98 , 99 , 100 ]. Therefore, miRNAs, such as miR-320, can serve as innovative targets for anti-cancerous drugs [ 97 ].…”

Targeting Pancreatic Ductal Adenocarcinoma (PDAC)

“…Inverso-isomer peptides also work. Neuropilins are multi-functional co-receptors, involved in a variety of signalling pathways, and an anticancer target of their own [216,217], with much relevance also for PDAC [218,219]. Various peptides targeting NRP-1 have been tested against cancers other than PDAC [220,221].…”