Neuropeptides in the human superior cervical ganglion

Baffi, Judit; Görcs, Tamás J.; Slowik, F.; Horváth, Miklós; Lekka, N.; Pásztor, E.; Palkovits, Miklós

doi:10.1016/0006-8993(92)90591-v

Cited by 68 publications

(29 citation statements)

References 33 publications

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“…NPY was widely distributed in neurons throughout the porcine greater splanchnic nerve ganglia. NPY is a major co-transmitter in the sympathetic ganglia, which is in accordance with immunohistochemistry reports in neurons of the sympathetic ganglia in pig [11], newborn and young mice [13], sheep [14], rat neonates and pups [15], and humans [16]. Previous studies described various size of the population of NPY-IR neurons in sympathetic ganglia in different mammals: 80% in the porcine celiac-superior mesenteric ganglia (CSMG) [17], 80-85% in the superior cervical ganglion (SCG) and middle cervical ganglion (MCG) of the cats and dogs [22], 40-60% in porcine stellate ganglion (SG) [10], 5-10% in porcine thoracolumbar ganglia [11].…”

Section: Discussionsupporting

confidence: 87%

“…The role of neuropeptides as putative neurotransmitters has become an important consideration in the study of nervous system functions. Different neuropeptides have previously been reported to exist in neurons of paravertebral and prevertebral ganglia in various mammalian species, including vasoactive intestinal polypeptide (VIP) in the pig [10,11], cat [12], newborn and young mice [13], sheep [14], rat neonates and pups [15], and humans [16]; encephalin (MEAGL) in the pig [10,11]; neuropeptide Y (NPY) in the pig [10,11], cat [12], newborn and young mice [13], sheep [14], rat neonates and pups [15], and humans [16]; substance P (SP) in the pig [10,11], cat [12], sheep [14], and humans [16]; calcitonin gene-related peptide (CGRP) in the pig [10,11], cat [12], newborn and young mice [13], sheep [14], and rat neonates and pups [15]; galanin (GAL) in the newborn and very young mice [13], sheep [14], rat neonates and pups [15], and pig [17]; somatostatin (SOM) in the pig [10,11], cat [12], rat neonates and pups [15], humans [16], and newborn and young mice [18]. In this study, attention has been focused on the neurochemical characteristics of neurons in the porcine greater splanchnic nerve, because until now the porcine GSN neurons have yet not been characterized by immunohistochemistry.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Nourinezhad

Podlasz

Wąsowicz

2018

Folia Histochem Cytobiol.

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Introduction. Greater splanchnic nerve (GSN) is by far the largest of the splanchnic nerves and connects the paravertebral and prevertebral ganglia to transmit the majority of nociceptive information from the viscera. Despite its importance, the immunohistochemical features of the porcine GSN neurons have not yet been examined. Therefore, the aim of the study was to investigate the neurochemistry of the porcine GSN neurons and to compare their neurochemical coding with those of the paravertebral and prevertebral ganglia. Material and methods. Four gilts of Large White Polish breed were examined in this study. Antibodies to tyrosine hydroxylase (TH), dopamine b-hydroxylase (DBH), choline acetyltransferase (ChAT), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), somatostatin (SOM), galanin (GAL), methionine-enkephalin (MET), calcitonin gene-related peptide (CGRP), and substance P (SP) were used for immunohistochemical detection of classical neurotransmitters marker enzymes and neuropeptides in neuronal cell bodies of the GSN. Results. Double-labeling immunofluorescence revealed that virtually all GSN neurons exhibited the presence of catecholamine-synthesizing enzymes (TH/DBH-positive) and subpopulations of neurons contained immunoreactivity to NPY, VIP, SOM, GAL and MET. However, CGRP and SP-immunoreactivity were not observed in neuronal somata. Conclusions. Our data strongly suggest that the general immunohistochemical characterization of ganglion cells in the porcine greater splanchnic nerve is similar to that of the prevertebral ganglia (e.g. celiacomesenteric ganglion).

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Nourinezhad

Podlasz

Wąsowicz

2018

Folia Histochem Cytobiol.

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“…After extensive washes and treatment with 1% sodium borohydride for 30 min (only for animals fixed with fixative B), the sections were incubated in the following antisera. For single or as a first immunostaining, rabbit anti-CCK (1:6000) , rabbit anti-parvalbumin (1:4000) (Baimbridge and Miller, 1982), or mouse antiparvalbumin (1:1000) (Sigma Immuno Chemicals, St. L ouis MO), or rabbit anti-V I P (1:10000) or rabbit anti-CGRP (1:10,000) (Baffi et al, 1992) or CB1 cannabinoid receptor (1:1000, Tsou et al, 1999) were used for 2 d at 4°C. In case of the FF X animals, the completeness of the lesion was checked using mouse anti-choline acetyl transferase antibody (CHAT; 1:300) (Cozzari et al, 1990).…”

Section: Methodsmentioning

confidence: 99%

Unusual Target Selectivity of Perisomatic Inhibitory Cells in the Hilar Region of the Rat Hippocampus

et al. 2000

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Perisomatic inhibitory innervation of all neuron types profoundly affects their firing characteristics and vulnerability. In this study we examined the postsynaptic targets of perisomatic inhibitory cells in the hilar region of the dentate gyrus where the proportion of potential target cells (excitatory mossy cells and inhibitory interneurons) is approximately equal. Both cholecystokinin (CCK)-and parvalbumin-immunoreactive basket cells formed multiple contacts on the somata and proximal dendrites of mossy cells. Unexpectedly, however, perisomatic inhibitory terminals arriving from these cell types largely ignored hilar GABAergic cell populations. Eighty-ninety percent of various GABAergic neurons including other CCK-containing basket cells received no input from CCK-positive terminals. Parvalbumin-containing cells sometimes innervated each other but avoided 75% of other GABAergic cells. Overall, a single mossy cell received 40 times more CCK-immunoreactive terminals and 15 times more parvalbumin-positive terminals onto its soma than the cell body of an average hilar GABAergic cell. In contrast to the pronounced target selectivity in the hilar region, CCK-and parvalbumin-positive neurons innervated each other via collaterals in stratum granulosum and moleculare.Our observations indicate that the inhibitory control in the hilar region is qualitatively different from other cortical areas at both the network level and the level of single neurons. The paucity of perisomatic innervation of hilar interneurons should have profound consequences on their action potential generation and on their ensemble behavior. These findings may help explain the unique physiological patterns observed in the hilus and the selective vulnerability of the hilar cell population in various pathophysiological conditions.

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“…The responses are mainly mediated by noradrenaline (NA) and neuropeptide Y (NPY) [48,49], at least 40-50% of the NA-positive cells contain NPY [12,186].…”

Section: Sympathetic Nervous Systemmentioning

confidence: 99%