Neuropeptide Y (NPY)-like immunoreactivity in rat sympathetic neurons and small granule-containing cells

Järvi, Riitta; Helén, Pauli; Pelto‐Huikko, Markku; Hervonen, Antti

doi:10.1016/0304-3940(86)90402-7

Cited by 89 publications

(33 citation statements)

References 16 publications

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“…Approximately 90% of rat SCG neurons belong to one of three functional groups: secretomotor neurons that project to the submandibular salivary glands, pilomotor neurons that innervate pilli arrector muscles, and vasomotor neurons that control blood flow in the head (Gibbins, 1991;Gibbins, 1995;Voyvodic, 1989). Neuropeptide Y (NPY) is an important cotransmitter in vasoconstrictor sympathetic neurons, which constitute 50-70% of the cells in paravertebral chain ganglia (Gibbins, 1995;Jarvi et al, 1986;Romeo et al, 1994). This suggests cellular variation in somatic staining for VMAT2 might correlate with the NPY-positive neuronal phenotype.…”

Section: Introductionmentioning

confidence: 99%

Different subcellular distributions of the vesicular monoamine transporter, VMAT2, in subclasses of sympathetic neurons

Headley¹,

Suhan²,

Horn³

2007

Brain Research

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A subpopulation of neurons in the rat superior cervical ganglion (SCG) was found to lack immunostaining for VMAT2, an isoform of the vesicular monoamine transporter that loads catecholamines into vesicles for release at the synapse. Double labeling with neuropeptide Y (NPY), a marker for vasomotor neurons, revealed selective cellular colocalization of NPY together with intense perinuclear staining for VMAT2. This implied that VMAT2-negative neurons were likely to have secretomotor and pilomotor phenotypes. We tested this by identifying peripheral noradrenergic axons by their expression of immunoreactivity for tyrosine hydroxylase (TH) and determining whether they also expressed NPY and VMAT2. This analysis revealed the presence of VMAT2-positive, non-vasomotor sympathetic axons in the submandibular gland and at the base of piloerector hairs. Together the results confirm earlier indications that virtually all sympathetic neurons in the rat SCG express VMAT2 and they show for the first time that functional subclasses of cells can be distinguished by different somatic levels of immunoreactivity for VMAT2.

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Section: Introductionmentioning

confidence: 99%

Different subcellular distributions of the vesicular monoamine transporter, VMAT2, in subclasses of sympathetic neurons

Headley¹,

Suhan²,

Horn³

2007

Brain Research

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“…Thus, the colocalization of NPY and noradrenaline is important for proper cardiovascular function. At birth and in the adult, about 60% of adult rat sympathetic superior cervical ganglion (SCG) neurons express NPY [Tyrrell and Landis, 1994;Jarvi et al, 1986;Lundberg et al, 1983], but the developmental regulation that results in noradrenergic sympathetic neurons with or without NPY is not well understood.…”

Section: Introductionmentioning

confidence: 99%

Influence of Non-Neuronal Cells on Establishment of Neuropeptide Y Expression by Sympathetic Neurons in vitro

MacPhedran

Hall²

2001

Dev Neurosci

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Sympathetic ganglion precursors were used to test the role of non-neuronal cell-derived factors in the establishment of mature neuronal phenotypes. In control conditions, characteristic neuronal populations with and without neuropeptide Y (NPY) expression developed after 1 week, but following removal of non-neuronal cells, virtually all neurons expressed NPY. Thus, ganglionic non-neuronal cells downregulated NPY expression in some but not all neurons in vitro. Conditioned medium from non-neuronal cells restored heterogeneous NPY expression, a finding that suggests the factors are soluble. A non-neuronal cell-derived cytokine, leukemia inhibitory factor, dramatically reduced NPY expression in all neurons, but no concentration tested could recapitulate heterogeneous neuronal NPY expression. Sympathetic precursors that incorporated BrdU early in the culture were more likely to develop NPY expression than neurons that became postmitotic later in vitro. Together, these findings support the notion that the environment in which neurons become postmitotic contributes to neuronal phenotype.

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“…In many instances, NPY is found to be colocalized with other neurotransmitters such as norepinephrine (Chronwall et al, 1984;Ekblad et al, 1984;Ever&t et al, 1984;Jarvi et al, 1986;Wang et al, 1987). NPY is only one member of a family of peptides that also includes peptide YY (PYY) and the pancreatic polypeptides (PP), as well as several others (Tatemoto, 1982b;Tatemoto et al, 1982).…”

mentioning

confidence: 99%

Neuropeptide Y modulates neurotransmitter release and Ca2+ currents in rat sensory neurons

et al. 1988

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Using 125I-labeled neuropeptide Y (NPY) and peptide YY (PYY), we demonstrated the existence of specific receptors for these peptides on rat dorsal root ganglion (DRG) cells grown in primary culture. Scatchard analysis of membrane homogenates indicated that the peptides bound to 2 populations of sites, with approximate affinities of 0.08 and 6.5 nM. Only low levels of binding were detected on sympathetic neurons cultured from the same animals or on a variety of neuronal clonal cell lines. The binding of 125I-NPY and 125I-PYY to DRG cell membranes was considerably reduced by the nonhydrolyzable analog of GTP, Gpp(NH)p. The major effect of Gpp(NH)p was to reduce the number of lower-affinity NPY binding sites without altering the number of high- affinity binding sites. NPY potently inhibited Ca2+ currents recorded under voltage clamp in rat DRG cells. Both the transient and sustained portions of the Ca2+ current were inhibited. The inhibitory effects of NPY were completely blocked following treatment of the cells with pertussis toxin. Depolarization elicited a large influx of Ca2+ into DRG neurons as assessed using fura-2-based microspectrofluorimetry. This influx of Ca2+ could be partially inhibited by NPY. Furthermore, NPY effectively inhibited the depolarization-induced release of substance P from DRG cells in vitro. Thus, NPY may be an important regulator of sensory neuron function in vivo.

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Neuropeptide Y (NPY)-like immunoreactivity in rat sympathetic neurons and small granule-containing cells

Cited by 89 publications

References 16 publications

Different subcellular distributions of the vesicular monoamine transporter, VMAT2, in subclasses of sympathetic neurons

Different subcellular distributions of the vesicular monoamine transporter, VMAT2, in subclasses of sympathetic neurons

Influence of Non-Neuronal Cells on Establishment of Neuropeptide Y Expression by Sympathetic Neurons in vitro

Neuropeptide Y modulates neurotransmitter release and Ca2+ currents in rat sensory neurons

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