Neuropeptide Y is an angiogenic factor in cardiovascular regeneration

Saraf, Rabya; Mahmood, Feroze; Amir, Rabia; Matyal, Robina

doi:10.1016/j.ejphar.2016.02.033

Cited by 38 publications

(34 citation statements)

References 68 publications

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“…20 On the other end, therapies that promote angiogenesis may contribute to cardiovascular regeneration, which is crucial to other disease such as diabetic cardiomyopathy and ischemic heart disease. 21,22 The in vivo enhancement of angiogenesis was also observed by others using biosynthesized gold nanoconjugates from plant extracts. 23 Thus, angiogenesis results from an intricate network of interactions between pro-and antiangiogenic molecules, endothelial cell receptors, and various modulators.…”

mentioning

confidence: 64%

Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo

Roma‐Rodrigues¹,

Heuer‐Jungemann²,

Fernandes³

et al. 2016

IJN

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In this work, peptides designed to selectively interact with cellular receptors involved in the regulation of angiogenesis were anchored to oligo-ethylene glycol-capped gold nanoparticles (AuNPs) and used to evaluate the modulation of vascular development using an ex ovo chick chorioallantoic membrane assay. These nanoparticles alter the balance between naturally secreted pro- and antiangiogenic factors, under various biological conditions, without causing toxicity. Exposure of chorioallantoic membranes to AuNP–peptide activators of angiogenesis accelerated the formation of new arterioles when compared to scrambled peptide-coated nanoparticles. On the other hand, antiangiogenic AuNP–peptide conjugates were able to selectively inhibit angiogenesis in vivo. We demonstrated that AuNP vectorization is crucial for enhancing the effect of active peptides. Our data showed for the first time the effective control of activation or inhibition of blood vessel formation in chick embryo via AuNP-based formulations suitable for the selective modulation of angiogenesis, which is of paramount importance in applications where promotion of vascular growth is desirable (eg, wound healing) or ought to be contravened, as in cancer development.

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mentioning

confidence: 64%

Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo

Roma‐Rodrigues¹,

Heuer‐Jungemann²,

Fernandes³

et al. 2016

IJN

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“…4b , p = 0.015), showing comparable levels to unstimulated controls. VEGF is a major angiogenic growth factor and is activated downstream of NPY signalling pathways 1 , 8 , 20 , 22 . The reduction in angiogenesis did not appear to be due to changes of NO levels released in ASC-derived conditioned media (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Neuropeptide Y (NPY) is a 36-amino acid peptide produced by the central nervous system and implicated in physiological neuron-mediated activities such as food intake or circadian rhythm 1 , 2 . In the autonomous nervous system NPY is stored in macrovesicles close to the varicosities present along the end terminals of sympathetic nerve fibres, where it is located in association with the adrenergic mediators.…”

Section: Introductionmentioning

confidence: 99%

“…Despite this heterogeneity, Y1, Y2 and Y5 receptors knockout mice similarly exhibit higher body weight, increased white adipose tissue and alteration of the cardiometabolic traits, suggesting that a multi- and combined receptor action exists 12 . Additionally, NPY exerts an anti-fibrotic potential in the ischemic heart 1 , 13 , thus contributing to tissue remodelling in the injured cardiac tissue. More importantly, NPY mediates the angiogenic process by enhancing endothelial cell proliferation in a calcium-dependent manner 14 .…”

Section: Introductionmentioning

confidence: 99%

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Platelet Lysate-Derived Neuropeptide y Influences Migration and Angiogenesis of Human Adipose Tissue-Derived Stromal Cells

Businaro

Scaccia

Bordin

et al. 2018

Sci Rep

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Neuropeptide Y (NPY), a powerful neurotransmitter of the central nervous system, is a key regulator of angiogenesis and biology of adipose depots. Intriguingly, its peripheral vascular and angiogenic powerful activity is strictly associated to platelets, which are source of clinical hemoderivates, such as platelet lysate (PL), routinely employed in several clinical applications as wound healing, and to preserve ex vivo the progenitor properties of the adipose stromal cells pool. So far, the presence of NPY in PL and its biological effects on the adipose stromal cell fraction (ASCs) have never been investigated. Here, we aimed to identify endogenous sources of NPY such as PL-based preparations and to investigate which biological properties PL-derived NPY is able to exert on ASCs. The results show that PL contains a high amount of NPY, which is in part also excreted by ASCs when stimulated with PL. The protein levels of the three main NPY subtype receptors (Y1, Y2, Y5) are unaltered by stimulation of ASCs with PL, but their inhibition through selective pharmacological antagonists, considerably enhances migration, and a parallel reduction of angiogenic features of ASCs including decrease in VEGF mRNA and intracellular calcium levels, both downstream targets of NPY. The expression of VEGF and NPY is enhanced within the sites of neovascularisation of difficult wounds in patients after treatment with leuco-platelet concentrates. Our data highlight the presence of NPY in PL preparations and its peripheral effects on adipose progenitors.

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“…Notable examples include trials for the chief angiogenic actors, VEGF 165 (the VIVA trial), and FGF‐2 (the FIRST trial) . While numerous other angiogenic factors, including, by way of recent examples, neuropeptide‐Y and hepatocyte growth factor, have yielded significant new vessel growth in animal models of MI, success will not be assured if translated to aged human patients, with their associated comorbidities. Even if successful in stimulating sufficient coronary angiogenesis, expansion of the capillary network without concomitant arteriogenesis would likely yield immature, unstable vessels which may later regress.…”

Section: Introductionmentioning

confidence: 99%

Prospects for improving neovascularization of the ischemic heart: Lessons from development

Smart

2017

Microcirculation

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Neovascularization of the ischemic myocardium postinfarction is necessary to restore blood flow to vulnerable cardiomyocytes and will be indispensable for prospective regenerative strategies, to perfuse newly formed myocardium. Therapeutic attempts to enhance new vessel formation have, to date, yielded modest clinical benefits, and innovative approaches are now needed. Intrinsic mechanisms are initiated by the heart in an attempt to rebuild injured vessels, but these are poorly understood. Insight into the underlying mechanisms may reveal targets for therapeutically augmenting this low-level neovascular response. Starting from a limited number of descriptive studies, this review summarizes what is known of coronary neovascularization and explores putative mechanisms and cellular sources which may endogenously contribute, or that may be pharmacologically triggered, to support vasculo- or angiogenesis. As injury responses in the adult frequently recapitulate embryological processes, a particular focus is placed on the developmental mechanisms of coronary vessel formation. An understanding of the cellular sources and the regulatory pathways used by the embryo may reveal novel targets for reactivating coronary vessel and myocardial regeneration.

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Neuropeptide Y is an angiogenic factor in cardiovascular regeneration

Cited by 38 publications

References 68 publications

Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo

Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo

Platelet Lysate-Derived Neuropeptide y Influences Migration and Angiogenesis of Human Adipose Tissue-Derived Stromal Cells

Prospects for improving neovascularization of the ischemic heart: Lessons from development

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