Neuropeptide Y facilitates P2X1 receptor-dependent vasoconstriction via Y1 receptor activation in small mesenteric arteries during sympathetic neurogenic responses

González-Montelongo, María del Carmen; Fountain, Samuel J.

doi:10.1016/j.vph.2020.106810

Cited by 11 publications

(20 citation statements)

References 54 publications

(59 reference statements)

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“…Frontiers in Physiology frontiersin.org Burnstock and Ralevic, 2014;Aalkjaer et al, 2021;Gonzalez-Montelongo and Fountain, 2021). The neurotransmitters used by vascular sensory nerves are calcitonin gene related peptide (CGRP), substance P, neurokinins, and, presumably, anandamide (Aalkjaer et al, 2021).…”

Section: Frontiers In Physiologymentioning

confidence: 99%

Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques

et al. 2023

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The study of the mechanisms of regulation of vascular tone is an urgent task of modern science, since diseases of the cardiovascular system remain the main cause of reduction in the quality of life and mortality of the population. Myography (isometric and isobaric) of isolated blood vessels is one of the most physiologically relevant approaches to study the function of cells in the vessel wall. On the one hand, cell-cell interactions as well as mechanical stretch of the vessel wall remain preserved in myography studies, in contrast to studies on isolated cells, e.g., cell culture. On the other hand, in vitro studies in isolated vessels allow control of numerous parameters that are difficult to control in vivo. The aim of this review was to 1) discuss the specifics of experimental design and interpretation of data obtained by myography and 2) highlight the importance of the combined use of myography with various complementary techniques necessary for a deep understanding of vascular physiology.

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Section: Frontiers In Physiologymentioning

confidence: 99%

Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques

et al. 2023

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“…Knockout studies in mice showed reduced fertility in male mice due to decreased vas deferens contractility, demonstrating the importance of the P2X1 receptor on the vas deferens [ 43 , 44 ]. In the cardiovascular system, P2X1 receptors are primarily located on arterial smooth muscle, and P2X1 receptor knockout mice showed a slight increase in blood pressure [ 1 , 42 , 44 , 45 ]. P2X1 receptors are also localised to immune cells such as platelets, macrophages, neutrophils and mast cells [ 1 , 42 , 46 , 47 ].…”

Section: P2x1 Receptorsmentioning

confidence: 99%

The P2X1 receptor as a therapeutic target

Bennetts

Mobbs

Ventura

et al. 2022

Purinergic Signalling

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Within the family of purinergic receptors, the P2X1 receptor is a ligand-gated ion channel that plays a role in urogenital, immune and cardiovascular function. Specifically, the P2X1 receptor has been implicated in controlling smooth muscle contractions of the vas deferens and therefore has emerged as an exciting drug target for male contraception. In addition, the P2X1 receptor contributes to smooth muscle contractions of the bladder and is a target to treat bladder dysfunction. Finally, platelets and neutrophils have populations of P2X1 receptors that could be targeted for thrombosis and inflammatory conditions. Drugs that specifically target the P2X1 receptor have been challenging to develop, and only recently have small molecule antagonists of the P2X1 receptor been available. However, these ligands need further biological validation for appropriate selectivity and drug-like properties before they will be suitable for use in preclinical models of disease. Although the atomic structure of the P2X1 receptor has yet to be determined, the recent discovery of several other P2X receptor structures and improvements in the field of structural biology suggests that this is now a distinct possibility. Such efforts may significantly improve drug discovery efforts at the P2X1 receptor.

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“…Typical effects mediated by Y 1 receptors include vasoconstriction in several vascular beds [ 2 ] including the mesenteric [ 5 , 6 , 7 ], renal [ 8 , 9 , 10 , 11 ], pulmonary [ 12 ] and cutaneous perfusion [ 13 ]. Y 1 receptors can also mediate the potentiation of vasoconstriction by other vasoactive agents [ 14 , 15 ]. Y 2 receptors have primarily been studied as prejunctional inhibitors of neurotransmitter release, both on sympathetic [ 16 , 17 ] and parasympathetic nerves [ 18 ] and in the brain [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, stimulation of NPY receptors often leads to elevation of the free intracellular Ca 2+ concentration, which occurs partly secondary to the activation of phospholipase C and partly by inositol phosphate-independent mobilization of Ca 2+ from intracellular stores [ 22 , 23 , 24 ] and by the influx of extracellular Ca 2+ via L-type channels [ 6 , 7 , 25 , 26 ]. The latter is often probed by using inhibitors of such channels such as nifedipine [ 6 , 15 , 27 , 28 , 29 ]. However, in contrast to Y 1 receptors, Y 2 receptor stimulation can inhibit voltage-dependent Ca 2+ channels [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Nifedipine on Renal and Cardiovascular Responses to Neuropeptide Y in Anesthetized Rats

Bischoff¹,

Stickan-Verfürth²,

Michel

2021

Molecules

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Neuropeptide Y (NPY) acts via multiple receptor subtypes termed Y1, Y2 and Y5. While Y1 receptor-mediated effects, e.g., in the vasculature, are often sensitive to inhibitors of L-type Ca2+ channels such as nifedipine, little is known about the role of such channels in Y5-mediated effects such as diuresis and natriuresis. Therefore, we explored whether nifedipine affects NPY-induced diuresis and natriuresis. After pre-treatment with nifedipine or vehicle, anesthetized rats received infusions or bolus injections of NPY. Infusion NPY (1 µg/kg/min) increased diuresis and natriuresis, and this was attenuated by intraperitoneal injection of nifedipine (3 µg/kg). Concomitant decreases in heart rate and reductions of renal blood flow were not attenuated by nifedipine. Bolus injections of NPY (0.3, 1, 3, 10 and 30 μg/kg) dose-dependently increased mean arterial pressure and renovascular vascular resistance; only the higher dose of nifedipine (100 μg/kg/min i.v.) moderately inhibited these effects. We conclude that Y5-mediated diuresis and natriuresis are more sensitive to inhibition by nifedipine than Y1-mediated renovascular effects. Whether this reflects a general sensitivity of Y5 receptor-mediated responses or is specific for diuresis and natriuresis remains to be investigated.

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Neuropeptide Y facilitates P2X1 receptor-dependent vasoconstriction via Y1 receptor activation in small mesenteric arteries during sympathetic neurogenic responses

Cited by 11 publications

References 54 publications

Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques

Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques

The P2X1 receptor as a therapeutic target

Effects of Nifedipine on Renal and Cardiovascular Responses to Neuropeptide Y in Anesthetized Rats

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