Neuropeptide-like protein diversity in phylum Nematoda

McVeigh, Paul; Alexander-Bowman, Susan J.; Veal, Emma; Mousley, Angela; Marks, Nikki J.; Maule, Aaron G.

doi:10.1016/j.ijpara.2008.05.006

Cited by 58 publications

(59 citation statements)

References 39 publications

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“…The double dash lines represent the way of sectioning (color Wgure online) multiple factors. These NLPs in C. elegans are known to be involved in neuronal signaling, development and antimicrobial activities (Nathoo et al 2001;Couillault et al 2004;McVeigh et al 2008). Whether these molecules have a similar function in the zebra mussels and are, therefore, regulated due to their importance in development, sensation, antimicrobial activities, is currently unknown and deserves additional studies.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling during the byssogenesis of zebra mussel (Dreissena polymorpha)

Faisal

2010

Mol Genet Genomics

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Since its invasion to the North American waters 20 years ago, the zebra mussel (Dreissena polymorpha) has negatively impacted the ecosystems through its firm underwater adhesion. The molecular mechanisms governing the functions of the zebra mussel byssus, the main structure responsible for maintaining the underwater adhesion, have received little attention. Our previously developed zebra mussel foot byssus cDNA microarray was applied in this study to identify the genes involved in different stages of the byssal threads generation. Byssal threads of zebra mussels were manually severed under laboratory conditions and the formation of new byssal threads was followed over a 3 week course. By comparing the gene expression profiles in different stages of byssal threads generation (byssogenesis) to their baseline values, we found that the number of unique byssus genes differentially expressed at 12-h, 1, 2, 3, 4, 7, and 21 days post-treatment was 13, 13, 20, 17, 16, 20, and 29, respectively. Comparisons were also made between two subsequent samples (e.g., 12 h vs. 1, 1 vs. 2 days, 2 vs. 3 days, and so on). Seven differentially expressed genes were selected for validation by using quantitative reverse transcription PCR (qRT-PCR) and the results were consistent with those from the microarray analysis. By using fluorescent in situ hybridization, we found that two microarray identified genes, BG15_F03-DPFP and BG16_H05-EGP, were expressed in two major byssus glands located in the zebra mussel foot: the stem-forming gland and plaque-forming gland, respectively. Moreover, the qRT-PCR of seven microarray identified genes with different zebra mussel samples suggested that they were also expressed in other mussel tissues beside the foot, albeit at much lower levels. This suggested that the microarray identified genes were produced primarily by the foot, and were likely associated with byssogenesis. The differentially expressed genes identified in this study indicated that multiple molecules are involved in byssogenesis, most likely performing multiple functions during the generation of byssal threads. These results obtained herein represent the first logical step toward understanding underwater attachment mechanisms employed by this invasive species.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling during the byssogenesis of zebra mussel (Dreissena polymorpha)

Faisal

2010

Mol Genet Genomics

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“…An additional positive quality of neuropeptides is their characteristically high potency when acting on cognate receptors [13, 25–30]. Furthermore, the high degree of phylogenetic sequence conservation suggests that neuropeptides could represent broad-spectrum nematicides as they share significant sequence similarity within and between parasite species [17, 22, 31, 32]. Disrupting PPN behaviour through the dysregulation of native neuropeptide signalling could hinder the development of resistance traits anchored on target receptor mutation.…”

Section: Introductionmentioning

confidence: 99%

Nematode neuropeptides as transgenic nematicides

et al. 2017

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Plant parasitic nematodes (PPNs) seriously threaten global food security. Conventionally an integrated approach to PPN management has relied heavily on carbamate, organophosphate and fumigant nematicides which are now being withdrawn over environmental health and safety concerns. This progressive withdrawal has left a significant shortcoming in our ability to manage these economically important parasites, and highlights the need for novel and robust control methods. Nematodes can assimilate exogenous peptides through retrograde transport along the chemosensory amphid neurons. Peptides can accumulate within cells of the central nerve ring and can elicit physiological effects when released to interact with receptors on adjoining cells. We have profiled bioactive neuropeptides from the neuropeptide-like protein (NLP) family of PPNs as novel nematicides, and have identified numerous discrete NLPs that negatively impact chemosensation, host invasion and stylet thrusting of the root knot nematode Meloidogyne incognita and the potato cyst nematode Globodera pallida. Transgenic secretion of these peptides from the rhizobacterium, Bacillus subtilis, and the terrestrial microalgae Chlamydomonas reinhardtii reduce tomato infection levels by up to 90% when compared with controls. These data pave the way for the exploitation of nematode neuropeptides as a novel class of plant protective nematicide, using novel non-food transgenic delivery systems which could be deployed on farmer-preferred cultivars.

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“…Several classes of effector proteins, such as lysozymes [18], neuropeptide-like proteins [19,20], caenacins [21], thaumatins [22], lipases [23], saposin-like proteins (caenopores) [24][25][26], medritin-like Shk toxins [3], proteins containing CUB-like domains [27,28], C-type lectins and collagens [29,30] have been identified as being involved in the interaction of C. elegans with microorganisms. Bioinformatic annotation of the C. elegans genome sequence assigns more than 750 genes to one of these protein families [31] which shows the diversity of potential effectors.…”

Section: Introductionmentioning

confidence: 99%