Neuronal systems immunoreactive with antiserum to lamprey gonadotropin-releasing hormone in the brain of Petromyzon marinus

King, Joan C.; Sower, Stacia A.; Anthony, Edythe L. P.

doi:10.1007/bf00221732

Cited by 56 publications

(46 citation statements)

References 17 publications

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“…Cerebrospinal fluidcontacting neurons containing immunoreactive GAL were reported before in teleosts (Holmqvist and Ekstrom, 1991), anurans (Lazar et al, 1991;Olivereau and Olivereau, 1992) and the turtle (Jimenez et al, 1994). In lampreys, CSFcontacting neurons have been found to be immunoreactive to antisera against several substances such as serotonin (Brodin et al, 1988a(Brodin et al, , 1990aPierre et al, 19921, somatostatin (Wright, 1986;YaAez et al, 1992), substance P (Nozaki and Gorbman, 19861, cholecystokinin (Brodin et al, 1988b), gonadotropin-releasing hormone (King et al, 1988), the molluscan cardio-excitatory tetrapeptide (Ohtomi et al, 1989) neurotensin (Brodin et al, 1990b), histamine (Brodin et al, 1990a), dopamine and tyrosine hydroxylase (Pierre et al, 19941, and opsins (Garcia-Fernandez and Foster, 1994). The brain of lamprey is considered to have conserved many characteristics of the primitive vertebrate brain (Nieuwenhuys, 1977).…”

Section: Hypothalamic Areasmentioning

confidence: 83%

Distribution of galanin‐like immunoreactive elements in the brain of the adult lamprey Lampetra fluviatilis

et al. 1996

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Galanin is a brain-gut peptide present in the central nervous system of vertebrates and invertebrates. The distribution of galanin-like immunoreactive perikarya and fibers in the brain of the river lamprey Lampetra fluviatilis (Agnatha) has been studied immunocytochemically by using antisera against rat and porcine galanin. Galanin-like immunoreactive perikarya were seen in the telencephalon and mediobasal diencephalon. In the telencephalon, they were present in the nucleus olfactorius anterior, nucleus basalis, and especially, in the nucleus commissurae anterioris. The diencephalon contained most of the immunoreactive neurons. They were located in the nucleus commissurae praeinfundibularis, nucleus ventralis hypothalami, nucleus commissurae postinfundibularis, nucleus ventralis thalami, and nucleus dorsalis thalami pars medius. Most of the galanin-like immunoreactive infundibular neurons showed apical processes contacting the cerebrospinal fluid. Immunoreactive fibers and terminals were widely distributed throughout the neuraxis. In the telencephalon, the richest galaninergic innervation was found in the nucleus olfactorius anterior, lobus subhippocampalis, corpus striatum, and around the nucleus septi and the nucleus praeopticus. In the diencephalon, the highest density of galanin-like immunoreactive fibers was seen in the nucleus commissurae postopticae, nucleus commissurae praeinfundibularis, nucleus ventralis hypothalami, nucleus dorsalis hypothalami, and neurohypophysis. In the mesencephalon and rhombencephalon, the distribution of immunoreactive fibers was heterogeneous, being most pronounced in a region between the nucleus nervi oculomotorii and the nucleus interpeduncularis mesencephali, in the nucleus isthmi, and in the raphe region. A subependymal plexus of immunoreactive fibers was found throughout the ventricular system. The distribution of immunoreactive neurons and fibers was similar to that of teleosts but different to those of other vertebrate groups. The possible hypophysiotropic and neuroregulatory roles of galanin are discussed.

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Section: Hypothalamic Areasmentioning

confidence: 83%

Distribution of galanin‐like immunoreactive elements in the brain of the adult lamprey Lampetra fluviatilis

et al. 1996

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“…Previously characterized antisera directed against lamprey GnRH-III, antiserum 3952 [Sower et al, 1993;Tobet et al, 1995], and lamprey GnRH-I, 1467 [King et al, 1988], were used at dilutions of 1/10,000 and 1/2,000, respectively. The lamprey GnRH-III antisera, 3952 was used in the pro-larval lamprey studies, whereas 3952 and antisera to lamprey GnRH-I, 1467, were used in the adult lamprey studies.…”

Section: Antibodies For Immunocytochemistrymentioning

confidence: 99%

“…The brains of the adult lampreys and whole heads from the developing lampreys were fixed in either 2% acrolein for immunocytochemistry [King et al, 1988], or 4% paraformaldehyde (PFA)/0.1 M phosphate buffer (PB) for in situ hybridization, pH 7.4 at room temperature (RT). The tissues were fixed overnight before transfer to 25% sucrose (w/v), 0.1 M PB for storage at 4°C.…”

Section: Animalsmentioning

confidence: 99%

The Spatial Relationship of γ-Aminobutyric Acid (GABA) Neurons and Gonadotropin-Releasing Hormone (GnRH) Neurons in Larval and Adult Sea Lamprey, <i>Petromyzon marinus</i>

et al. 2002

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In this study we examined the spatial relationship of GABA-containing and GnRH-containing neurons by immunocytochemistry and in situ hybridization in larval and adult brains of sea lamprey, Petromyzon marinus. In immunocytochemical studies, GABA-containing neurons were detected early in lamprey development, by day 20 post-fertilization. At this time point, one population of GABA-containing neurons was visualized in the hypothalamus and preoptic area, and another population was located in the olfactory bulb of the telencephalon. By day 30 after fertilization, after the GABA neurons were detected, GnRH-containing neurons were visualized in the preoptic area/rostral hypothalamus region, adjacent to the GABA-containing neurons in the wall of the third ventricle. Similarly, in adult lamprey brains distinct populations of both GABA- and GnRH-containing neurons were located in the hypothalamus adjacent to the third ventricle. To further establish a proximate relationship between GABA and GnRH, the mRNA for glutamate decarboxylase (GAD), the enzyme catalyzing GABA synthesis from glutamate, and GnRH were examined by in situ hybridization in the brains of larval lamprey. These studies also showed that GnRH and GAD are produced in cell populations in and around the third ventricle of the hypothalamus. This close spatial relationship of GABA neurons and GnRH neurons provides a basis for a regulatory role of GABA on GnRH neurons in the sea lamprey.

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“…Numerous immunocytochemistry (ICC) studies [Crim et al, 1979a, b;Nozaki and Kobayashi, 1979;Nozaki et al, 1984Nozaki et al, , 2000King et al, 1988;Wright et al, 1994;Tobet et al, 1995Tobet et al, , 1996Reed et al, 2002] have investigated the distribution of lamprey GnRH in the brain of several lamprey species. These studies have consistently demonstrated the localization of lamprey GnRH-I and -III in the preoptic area and hypothalamus extending to the neurohypophysis.…”

Section: Introductionmentioning

confidence: 99%

In situ Characterization of Gonadotropin- Releasing Hormone-I, -III, and Glutamic Acid Decarboxylase Expression in the Brain of the Sea Lamprey, <i>Petromyzon marinus</i>

et al. 2004

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The distribution of lamprey gonadotropin-releasing hormone (GnRH)-I and -III has been extensively characterized by immunocytochemistry in the forebrain of the sea lamprey, Petromyzon marinus. However, the cellular location of lamprey GnRH-III mRNA expression by in situ hybridization in the lamprey brain has not been determined. We show for the first time the location of expression of lamprey GnRH-III, as well as provide a more comprehensive in situ study of lamprey GnRH-I and glutamic acid decarboxylase (GAD; GABA-synthesizing enzyme) mRNA expression in the brain of the lamprey in different reproductive life stages. Colorimetric and dual-label fluorescent amplification methods of in situ hybridization were used on brain tissue sections of adult, juvenile, and larval sea lamprey. In each life stage of the lamprey, expression of lamprey GnRH-I was shown in the preoptic area (POA) and the hypothalamus forming the characteristic arc-like cell population extending from the preoptic nucleus (NPO) to the neurohypophysis. Lamprey GnRH-III expression was also seen in the POA of each life stage in close proximity to lamprey GnRH-I mRNA containing neurons. GAD expression was shown in distinct cell clusters in and around the POA, in the olfactory bulb, in the dorsal thalamus beneath the habenular region, and also in the ventral-medial hypothalamus stretching from the periventricular region to the anterior portion of the rhombencephalon. Using dual-label in situ hybridization, we have shown that lamprey GnRH-I and -III mRNA are colocalized in the same cells in the POA in adult lampreys. Dual-label in situ hybridization also showed close proximity of GAD mRNA containing neurons and GnRH containing neurons in the POA. These data suggest that γ-aminobutyric acid (GABA) may directly affect GnRH release in the brain of the sea lamprey.

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Neuronal systems immunoreactive with antiserum to lamprey gonadotropin-releasing hormone in the brain of Petromyzon marinus

Cited by 56 publications

References 17 publications

Distribution of galanin‐like immunoreactive elements in the brain of the adult lamprey Lampetra fluviatilis

Distribution of galanin‐like immunoreactive elements in the brain of the adult lamprey Lampetra fluviatilis

The Spatial Relationship of γ-Aminobutyric Acid (GABA) Neurons and Gonadotropin-Releasing Hormone (GnRH) Neurons in Larval and Adult Sea Lamprey, <i>Petromyzon marinus</i>

In situ Characterization of Gonadotropin- Releasing Hormone-I, -III, and Glutamic Acid Decarboxylase Expression in the Brain of the Sea Lamprey, <i>Petromyzon marinus</i>

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