Neuronal Survival and Glial Reactions after Recurrent Laryngeal Nerve Resection in the Rat

Hydman, Jonas; Svensson, Mikael; Kuylenstierna, Richard; Ohlsson, Marcus; Mattsson, Per

doi:10.1097/01.mlg.0000161362.43320.b2

Cited by 19 publications

(43 citation statements)

References 16 publications

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“…This phenomenon was not adjusted for, because our earlier results showed that a similar injury (RLN resection) in the same system causes no neuronal loss. 18 Analysis of PCA Muscle Sections. Muscle sections were analyzed using a Leica confocal system (TCS SP2) connected to a Leica DMIRE2 microscope with a 63ϫ/1.40 -0.60 objective.…”

Section: Quantification Of Retrogradely Traced Pca Neuronsmentioning

confidence: 99%

Collateral reinnervation by the superior laryngeal nerve after recurrent laryngeal nerve injury

Hydman

Mattsson

2008

Muscle and Nerve

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This study investigates the role of the intact superior laryngeal nerve (SLN) in the reinnervation process of one of the laryngeal muscles, the posterior cricoarytenoid muscle (PCA), following recurrent laryngeal nerve (RLN) injury. Using a chronic RLN injury model in the adult rat, PCA reinnervation was assessed by retrograde double-tracing techniques in combination with electrophysiology and immunohistochemistry of muscle sections. The results demonstrate that the PCA receives dual innervation from both laryngeal nerves even in the uninjured system. Functionally significant collateral reinnervation originates from intact SLN fibers following RLN injury, mainly due to intramuscular sprouting rather than by recruitment of more motor neurons. This may be important when choosing surgical and/or medical treatment for patients with RLN injury.

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Section: Quantification Of Retrogradely Traced Pca Neuronsmentioning

confidence: 99%

Collateral reinnervation by the superior laryngeal nerve after recurrent laryngeal nerve injury

Hydman

Mattsson

2008

Muscle and Nerve

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“…They may be summarized by cortical contact, neuronal survival, pace of regeneration, misguidance, and degree of muscle atrophy. After injury to the RLN, there have been speculations about misguidance into the adductor and abductor branches of the nerve causing the loss of functional restitution, hampered coordination, or synkinesis of the vocal cord 6 . There is also evidence of rapid occupation of motor end plates in the larynx muscles by autonomous fibers after RLN injury 7 .…”

Section: Introductionmentioning

confidence: 99%

Nimodipine and Microsurgery Induced Recovery of the Vocal Cord after Recurrent Laryngeal Nerve Resection

et al. 2005

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Transection of the recurrent laryngeal nerve leads to permanent palsy of the vocal cord. Experimental studies have confirmed that nimodipine increases the pace of axonal regeneration. We present a case of a 19-year-old male, suffering a thyroid cancer disease, who was subjected to unilateral resection of the recurrent laryngeal nerve during surgery. The nerve was repaired with a nerve graft and the patient further treated with nimodipine for 3 months. Evaluation of the patient showed normalization of voice, movement of the vocal cord on the injured side, and electromyography evidence of reinnervation of the larynx muscles at 15 months after surgery.

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“…These findings suggest that the endogenous NPCs attempt to repair the lesioned NA. It is generally known that a retrograde axonal reaction can be caused by injury of a peripheral motor nerve [14]. Decreased motor neuron survival numbers, reactive microglia, astrocytes and macrophages were observed after the RLN avulsion injury in our previous report [12].…”

Section: Discussionmentioning

confidence: 66%

Migration and Differentiation of Neural Progenitor Cells after Recurrent Laryngeal Nerve Avulsion in Rats

Zhao

2014

PLoS ONE

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To investigate migration and differentiation of neural progenitor cells (NPCs) from the ependymal layer to the nucleus ambiguus (NA) after recurrent laryngeal nerve (RLN) avulsion. All of the animals received a CM-DiI injection in the left lateral ventricle. Forty-five adult rats were subjected to a left RLN avulsion injury, and nine rats were used as controls. 5-Bromo-2-deoxyuridine (BrdU) was injected intraperitoneally. Immunohistochemical analyses were performed in the brain stems at different time points after RLN injury. After RLN avulsion, the CM-DiI+ NPCs from the ependymal layer migrated to the lesioned NA. CM-DiI+/GFAP+ astrocytes, CM-DiI+/DCX+ neuroblasts and CM-DiI+/NeuN+ neurons were observed in the migratory stream. However, the ipsilateral NA included only CM-DiI+ astrocytes, not newborn neurons. After RLN avulsion, the NPCs in the ependymal layer of the 4th ventricle or central canal attempt to restore the damaged NA. We first confirm that the migratory stream includes both neurons and glia differentiated from the NPCs. However, only differentiated astrocytes are successfully incorporated into the NA. The presence of both cell types in the migratory process may play a role in repairing RLN injuries.

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Neuronal Survival and Glial Reactions after Recurrent Laryngeal Nerve Resection in the Rat

Cited by 19 publications

References 16 publications

Collateral reinnervation by the superior laryngeal nerve after recurrent laryngeal nerve injury

Collateral reinnervation by the superior laryngeal nerve after recurrent laryngeal nerve injury

Nimodipine and Microsurgery Induced Recovery of the Vocal Cord after Recurrent Laryngeal Nerve Resection

Migration and Differentiation of Neural Progenitor Cells after Recurrent Laryngeal Nerve Avulsion in Rats

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