Neuronal SNAP-23 is critical for synaptic plasticity and spatial memory independently of NMDA receptor regulation

Huang, Mengjia; Bin, Na-Ryum; Rai, Jayant; Ma, Ke; Chow, Chung‐Wai; Eide, Sarah; Harada, Hirofumi; Xiao, Jianbing; Feng, Daorong; Sun, Hong‐Shuo; Feng, Zhong‐Ping; Gaisano, Herbert Y.; Pessin, Jeffrey E.; Monnier, Philippe P.; Okamoto, Kenichi; Zhang, Liang; Sugita, Shuzo

doi:10.1016/j.isci.2023.106664

Cited by 5 publications

(2 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SNAREs contain one R-and three Q-SNARE (subdivided into Qa, Qb, and Qc) domains. SNAP-25(Synaptosomal-Associated Protein 25) and its paralogs SNAP-23, important subfamily members of SNAREs with the Qb and Qc domains, are specialized for driving regulated exocytosis [17][18][19]. Different from the neuron-specific SNAP-25, SNAP-23 is ubiquitously expressed in the blood and immune system, the musculoskeletal system, most internal organs, secretory and reproductive organs as well as in the brain [17,20].…”

Section: Snap23mentioning

confidence: 99%

Identification of Key Genes and Imbalanced SNAREs Assembly in the Comorbidity of Polycystic Ovary Syndrome and Depression

Cao,

Wang,

Song

et al. 2024

Genes

View full text Add to dashboard Cite

Background: Women with polycystic ovary syndrome (PCOS) have increased odds of concurrent depression, indicating that the relationship between PCOS and depression is more likely to be comorbid. However, the underlying mechanism remains unclear. Here, we aimed to use bioinformatic analysis to screen for the genetic elements shared between PCOS and depression. Methods: Differentially expressed genes (DEGs) were screened out through GEO2R using the PCOS and depression datasets in NCBI. Protein–protein interaction (PPI) network analysis and enrichment analysis were performed to identify the potential hub genes. After verification using other PCOS and depression datasets, the associations between key gene polymorphism and comorbidity were further studied using data from the UK biobank (UKB) database. Results: In this study, three key genes, namely, SNAP23, VTI1A, and PRKAR1A, and their related SNARE interactions in the vesicular transport pathway were identified in the comorbidity of PCOS and depression. The rs112568544 at SNAP23, rs11077579 and rs4458066 at PRKAR1A, and rs10885349 at VTI1A might be the genetic basis of this comorbidity. Conclusions: Our study suggests that the SNAP23, PRKAR1A, and VTI1A genes can directly or indirectly participate in the imbalanced assembly of SNAREs in the pathogenesis of the comorbidity of PCOS and depression. These findings may provide new strategies in diagnosis and therapy for this comorbidity.

show abstract

Section: Snap23mentioning

confidence: 99%

Identification of Key Genes and Imbalanced SNAREs Assembly in the Comorbidity of Polycystic Ovary Syndrome and Depression

Cao,

Wang,

Song

et al. 2024

Genes

View full text Add to dashboard Cite

show abstract

“…SNAP-23 and SNAP-25 flox mice were created as described previously (Feng et al, 2018;Hoerder-Suabedissen et al, 2019;Huang et al, 2023;Liang et al, 2020;Marques-Smith et al, 2016). Briefly, loxP sites were introduced surrounding exon 3 of SNAP-23 for SNAP-23 flox mice and loxP sites surrounded exon 5 of SNAP-25 in SNAP-25 flox mice.…”

Section: Animalsmentioning

confidence: 99%

SNAP-25, but not SNAP-23, is essential for photoreceptor function and survival in mice

Huang,

Chow,

Gurdita

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Vesicular transport plays critical roles in photopigment delivery at photoreceptor outer segments and glutamate exocytosis at photoreceptor synapses. Previous studies into the role of photoreceptor SNAP proteins are limited in their characterizations into only gene/protein expression and do not delve further into their functional role. Here, we examine the expression and localization of SNAP-23 and SNAP-25 mRNA and protein. Using SNAP-23 and SNAP-25 conditional knockout mice, we further evaluated the morphological and functional consequences that the absence of these proteins has on vision. Although we found that the ubiquitously expressed SNAP-23 showed weak mRNA expression in photoreceptors, removal of SNAP-23 did not result in any observable phenotype. We found that neuronal SNAP-25 is developmentally regulated and SNAP-25 mRNA undergoes mRNA trafficking to the photoreceptor inner segments coinciding with the development of photoreceptor outer segments. Removal of SNAP-25 in photoreceptor cells led to changes in both outer segment protein trafficking and synaptic integrity, resulting in a complete loss of vision in SNAP-25 cKO mice. Our results conclude that SNAP-25, but not SNAP-23, is the essential isoform for photoreceptor survival and function.

show abstract

SNAP-25, but not SNAP-23, is essential for photoreceptor development, survival, and function in mice

Huang,

Chow,

Gurdita

et al. 2024

Commun Biol

View full text Add to dashboard Cite

SNARE-mediated vesicular transport is thought to play roles in photoreceptor glutamate exocytosis and photopigment delivery. However, the functions of Synaptosomal-associated protein (SNAP) isoforms in photoreceptors are unknown. Here, we revisit the expression of SNAP-23 and SNAP-25 and generate photoreceptor-specific knockout mice to investigate their roles. Although we find that SNAP-23 shows weak mRNA expression in photoreceptors, SNAP-23 removal does not affect retinal morphology or vision. SNAP-25 mRNA is developmentally regulated and undergoes mRNA trafficking to photoreceptor inner segments at postnatal day 9 (P9). SNAP-25 knockout photoreceptors develop normally until P9 but degenerate by P14 resulting in severe retinal thinning. Photoreceptor loss in SNAP-25 knockout mice is associated with abolished electroretinograms and vision loss. We find mistrafficked photopigments, enlarged synaptic vesicles, and abnormal synaptic ribbons which potentially underlie photoreceptor degeneration. Our results conclude that SNAP-25, but not SNAP-23, mediates photopigment delivery and synaptic functioning required for photoreceptor development, survival, and function.

show abstract

Neuronal SNAP-23 is critical for synaptic plasticity and spatial memory independently of NMDA receptor regulation

Cited by 5 publications

References 59 publications

Identification of Key Genes and Imbalanced SNAREs Assembly in the Comorbidity of Polycystic Ovary Syndrome and Depression

Identification of Key Genes and Imbalanced SNAREs Assembly in the Comorbidity of Polycystic Ovary Syndrome and Depression

SNAP-25, but not SNAP-23, is essential for photoreceptor function and survival in mice

SNAP-25, but not SNAP-23, is essential for photoreceptor development, survival, and function in mice

Contact Info

Product

Resources

About