Neuronal regeneration in a zebrafish model of adult brain injury

Kishimoto, Norihito; Shimizu, Kanichiro; Sawamoto, Kazunobu

doi:10.1242/dmm.007336

Cited by 193 publications

(211 citation statements)

References 72 publications

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“…However, other species such as dogs [10], pigs [11], sheep [12], cats [13,14], zebrafish [15] and nonhuman primates (NHP) have been used to develop animal models of CNS injuries [16,17]. assess sensory acuity with greater parallels to functional deficits observed in human patients.…”

Section: Current Models Of Cns Injury Research: the Importance Of Thementioning

confidence: 99%

Models of CNS injury in the nonhuman primate: A new era for treatment strategies

Teo

Rosenfeld²,

Bourne

2012

Translational Neuroscience

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Central nervous system (CNS) injuries affect all levels of society indiscriminately, resulting in functional and behavioral deficits with devastating impacts on life expectancies, physical and emotional wellbeing. Considerable literature exists describing the pathophysiology of CNS injuries as well as the cellular and molecular factors that inhibit regrowth and regeneration of damaged connections. Based on these data, numerous therapeutic strategies targeting the various factors of repair inhibition have been proposed and on-going assessment has demonstrated some promising results in the laboratory environ. However, several of these treatment strategies have subsequently been taken into clinical trials but demonstrated little to no improvement in patient outcomes. As a result, options for clinical interventions following CNS injuries remain limited and effective restorative treatment strategies do not as yet exist. This review discusses some of the current animal models, with focus on nonhuman primates, which are currently being modeled in the laboratory for the study of CNS injuries. Last, we review the current understanding of the mechanisms underlying repair/regrowth inhibition and the current trends in experimental treatment strategies that are being assessed for potential translation to clinical applications.

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Section: Current Models Of Cns Injury Research: the Importance Of Thementioning

confidence: 99%

Models of CNS injury in the nonhuman primate: A new era for treatment strategies

Teo

Rosenfeld²,

Bourne

2012

Translational Neuroscience

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“…Adult neurogenesis has been observed in rodents and songbirds, where newborn neurons are added throughout life to highly restricted spatial regions in the telencephalon [5][6][7][8][9]. In vivo in mammals, endogenous Neural Progenitor Cells (NPCs), almost generated along the Sub Ventricular Zone (SVZ) in the adult brain, have been proposed as a potential source of newborn neurons for the neural tissue repair after various brain insults, such as ischemic stroke or traumatic brain injury [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…Dissimilarly to birds and mammals, low non-mammalian vertebrates, as fish, amphibians and reptiles, display a considerable amount of adult neurogenesis in more numerous brain regions, even though such differences are poorly understood and investigated [11]. The adult CNS of teleost fish exhibits a high capacity for neuronal regeneration after injury [4].…”

Section: Introductionmentioning

confidence: 99%

“…The adult CNS of teleost fish exhibits a high capacity for neuronal regeneration after injury [4]. Comparative studies in zebra fish and mammals show that the telencephalic Ventricular Zone (VZ) in the adult zebra fish brain generates NPCs that share characteristics with the NPCs in the mammalian SVZ: they migrate tangentially and differentiate into mature neurons into the Olfactory Bulb (OB) [5,[11][12][13][14][15]. Recently, Kishimoto et al [11] developed an in vivo fish model of telencephalic injury to demonstrate the strong ability of adult zebra fish to undergo neuronal regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Kishimoto et al [11] developed an in vivo fish model of telencephalic injury to demonstrate the strong ability of adult zebra fish to undergo neuronal regeneration. Prodigious regenerative processes were found in the injured zebra fish telencephalon, with the proliferation of endogenous NPCs, their lateral migration and neuronal differentiation at the injury site [11].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multilineage Differentiation Potential of CNS Cell Progenitors in a Recent Developed Gilthead Seabream (Sparus aurata L.) Nervous Model

Santacroce¹

2014

J Bioproces Biotech

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Neural Progenitor Cells (NPCs) have gathered more and more attention in the field of Neural Stem Cells (NSCs). However, the multilineage differentiating behavior of these cells and their contribution to tissue regeneration, almost in lower vertebrate taxa, remain unknown. Since the early 1970s, many comparative studies have been performed using immunocytochemical screening on the brains of several vertebrate taxa, including teleosts, in order to identify these cells, even if the data are sometimes contrasting. This study aims: (1) to investigate in vitro the potential proliferative role of NPCs and Radial Glia Progenitors (RGP) in seabream neurogenesis; (2) to reveal the strict ability of fish NSCs to undertake the multilineage development and differentiation in neurons, astrocytes and oligodendrocytes.By the use of double Immunofluorescence (IF) analysis and phase contrast microscopy, we identified the multilineage differentiation and the exact cell morphology. We demonstrated that NSC can self-renew and differentiate into different types of neurons or glial cells during extended culturing. Mature neurons expressed specific neuronal markers; they could differentiate during long term culturing, generating an extensive neurite growth. Glia was found highly mitotic and could developed mature astrocytes and oligodendrocytes. Glial cells were assessed by Glial Fibrillary Acidic Protein (GFAP) reactivity; neurons and myelinating oligodendrocytes were immunostained with cell-specific markers.This work provide that the multilineage differentiation potential of seabream neural cell progenitors might be a useful tool for neurodegenerative diseases, being a promising approach for repairing the CNS injuries, also in other animals, as a new coming strategy for function recovery of damaged nerves.

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Neurogenesis is required for behavioral recovery after injury in the visual system of Xenopus laevis

McKeown¹,

Sharma²,

Sharipov³

et al. 2013

J of Comparative Neurology

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Non-mammalian vertebrates have a remarkable capacity to regenerate brain tissue in response to CNS injury. Nevertheless, it is not clear whether animals recover lost function after injury or whether injury-induced cell proliferation mediates recovery. We address these questions using the visual system and visually-guided behavior in Xenopus laevis tadpoles. We established a reproducible means to produce a unilateral focal injury to optic tectal neurons without damaging retinotectal axons. We then assayed a tectally-mediated visual avoidance behavior to evaluable behavioral impairment and recovery. Focal ablation of part of the optic tectum prevents the visual avoidance response to moving stimuli. Animals recover the behavior over the week following injury. Injury induces a burst of proliferation of tectal progenitor cells based on phospho-histone H3 immunolabeling and experiments showing that Musashi-immunoreactive tectal progenitors incorporate the thymidine analog iododeoxyuridine after injury. Pulse chase experiments indicate that the newly-generated cells differentiate into N-β–tubulin-immunoreactive neurons. Furthermore, in vivo time-lapse imaging shows that Sox2-expressing neural progenitors divide in response to injury and generate neurons with elaborate dendritic arbors. These experiments indicate that new neurons are generated in response to injury. To test if neurogenesis is necessary for recovery from injury, we blocked cell proliferation in vivo and found that recovery of the visual avoidance behavior is inhibited by drugs that block cell proliferation. Moreover, behavioral recovery is facilitated by changes in visual experience that increase tectal progenitor cell proliferation. Our data indicate that neurogenesis in the optic tectum is critical for recovery of visually-guided behavior after injury.

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Neuronal regeneration in a zebrafish model of adult brain injury

Cited by 193 publications

References 72 publications

Models of CNS injury in the nonhuman primate: A new era for treatment strategies

Models of CNS injury in the nonhuman primate: A new era for treatment strategies

Multilineage Differentiation Potential of CNS Cell Progenitors in a Recent Developed Gilthead Seabream (Sparus aurata L.) Nervous Model

Neurogenesis is required for behavioral recovery after injury in the visual system of Xenopus laevis

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