Neuronal polarity selection by topography-induced focal adhesion control

Ferrari, Aldo; Cecchini, Marco; Serresi, Michela; Faraci, Paolo; Pisignano, Dario; Beltram, Fabio

doi:10.1016/j.biomaterials.2010.02.032

Cited by 109 publications

(167 citation statements)

References 45 publications

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“…Their optical properties and thickness (0.18 mm) allow high-resolution imaging of living cells [29]. The nanoimprinting protocol yielded reproducible GRs, with 50% duty cycle (i.e.…”

Section: Resultsmentioning

confidence: 99%

“…In particular, in the last few years nanogratings-anisotropic topographies composed of alternating lines of grooves and ridges with submicrometre lateral dimensions-have been intensively investigated and have emerged as one of the most effective systems for inducing neuronal alignment via pure cell mechanotransduction. In previous studies, we showed that the interaction of nerve growth factor (NGF)-differentiating PC12 cells with these substrates promotes bipolarity and alignment to the substrate topography by interfering with FA establishment and maturation [29][30][31]. The ability to direct neurite growth depends on both the dimensions of the underlying patterned substrate and, very importantly, on the neuronal cell type [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

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Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

et al. 2014

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Controlling and improving neuronal cell migration and neurite outgrowth are critical elements of tissue engineering applications and development of artificial neuronal interfaces. To this end, a promising approach exploits nano/microstructured surfaces, which have been demonstrated to be capable of tuning neuronal differentiation, polarity, migration and neurite orientation. Here, we investigate the neurite contact guidance of leech neurons on plastic gratings (GRs; anisotropic topographies composed of alternating lines of grooves and ridges). By high-resolution microscopy, we quantitatively evaluate the changes in tubulin cytoskeleton organization and cell morphology and in the neurite and growth cone development. The topography-reading process of leech neurons on GRs is mediated by filopodia and is more responsive to 4-µm-period GRs than to smaller period GRs. Leech neuron behaviour on GRs is finally compared and validated with several other neuronal cells, from murine differentiated embryonic stem cells and primary hippocampal neurons to differentiated human neuroblastoma cells.

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“…Their optical properties and thickness (0.18 mm) allow high-resolution imaging of living cells [29]. The nanoimprinting protocol yielded reproducible GRs, with 50% duty cycle (i.e.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

et al. 2014

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“…Contact guidance is responsible for these effects. This process is based on the physical confinement of transmembrane receptors of the integrin family, which controls biological contact between the cell and its substrate through the establishment and maturation of focal adhesions [15,18]. Less attention has been paid so far to the influence of topography on entire monolayers as opposed to individual cells [19].…”

Section: Introductionmentioning

confidence: 99%

Accelerated endothelial wound healing on microstructured substrates under flow

Franco¹,

Milde²,

Klingauf³

et al. 2013

Biomaterials

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“…This clustering of focal adhesions on the side facing the aligned fibrils results in neurite persistence and aligned neurite outgrowth. 47,49 This finding could benefit in vivo strategies of spinal cord repair, as it would promote directional neurite outgrowth not only from within the scaffold, but also from aberrant growth outside of the scaffold.…”

Section: Contact Mediated Axon Guidancementioning

confidence: 99%

Nano-textured self-assembled aligned collagen hydrogels promote directional neurite guidance and overcome inhibition by myelin associated glycoprotein

Abu-Rub

Billiar

et al. 2011

Soft Matter

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The UCD community has made this article openly available. Please share how this access benefits you. Your story matters! (@ucd_oa) Some rights reserved. For more information, please see the item record link above. TitleNano-textured self-assembled aligned collagen hydrogels promote directional neurite guidance and overcome inhibition by myelin associated glycoprotein The development of nerve guidance conduits is constantly evolving as the need arises for therapies for spinal cord injury. In addition to providing a path for regrowing axons to reconnect with their appropriate targets, the structural and biochemical cues provided by these conduits should be permissive for directional neurite outgrowth and be protective against inhibition in the vicinity of the injury site. Here, we adapted the use of iso-electric focusing to drive the alignment of supramolecular fibrils into self-assembled collagen hydrogels ($300 mm diameter), and tested those hydrogels for the ability to direct and enhance the migration of neurites. Structural characterization revealed anisotropic alignment of nanofibrillar aggregates ($20 nm diameter), arranged in micron-scale bundles ($1 to 2 mm diameter) similar to the hierarchical size scales observed in native tissues. Neurite outgrowth extended bidirectionally along the axes of aligned hydrogels. Furthermore, it was shown that, as opposed to poly-D-lysine, neurite outgrowth on aligned hydrogels is not inhibited in the presence of myelin-associated glycoprotein (p > 0.05). These results highlight for the first time a structural and biochemical role for iso-electrically aligned collagen hydrogels in controlling neuronal growth, and indicate that the short-term signaling associated with these hydrogels can be used in adjunct therapy following injury to the spinal cord.

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Neuronal polarity selection by topography-induced focal adhesion control

Cited by 109 publications

References 45 publications

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

Accelerated endothelial wound healing on microstructured substrates under flow

Nano-textured self-assembled aligned collagen hydrogels promote directional neurite guidance and overcome inhibition by myelin associated glycoprotein

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