Neuronal pathway involved in nitric oxide-mediated descending relaxation in rat ileum

Kanada, Atsunori; Hosokawa, Muneaki; Suthamnatpong, Naowarat; Maehara, Tomofumi; Takeuchi, Tadayoshi; Hata, Fumiaki

doi:10.1016/0014-2999(93)90621-n

Cited by 21 publications

(11 citation statements)

References 24 publications

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“…Participation of intrinsic cholinergic neurons in ascending and descending reflexes was reported in the guinea pig midcolon (Grider and Makhlouf, 1986). Participation of the neurons in descending relaxation was also reported in the rat proximal colon and ileum (Kanada et al, 1993). The role of cholinergic interneurons in an ascending excitatory neural pathway was also suggested in the guinea-pig distal colon by mechanical recording of peristalsis generated by slow filling of the colonic lumen with fluid (Smith and Robertson, 1998).…”

Section: Introductionmentioning

confidence: 83%

Examination of the role of cholinergic myenteric neurons with the impairment of neural reflexes in the ileum of c-kit mutant mice

Okishio

Takeuchi

Fujita

et al. 2005

J. Smooth Muscle Res.

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Our previous study showed that impairment of ascending and descending neural reflexes in the ileum of the c-kit mutant, W/W V , mice is due to a loss of interstitial cells of Cajal present at the myenteric plexus region (ICC-MY) in the mutant. In the present study, cholinergic interneurons were thought to be involved in these pathways, since hexamethonium, an antagonist of the nicotinic ACh receptor, significantly inhibited both neural reflexes in wild type mice. Therefore, we examined whether the loss of ICC-MY affects cholinergic interneurons involved in these pathways. Immunohistochemistry with anti-choline acetyltransferase revealed that there was no difference in the numbers of immunopositive cells in the myenteric plexus region between the wild type and mutant mice. In addition, there was no difference in the extent of spontaneous and EFS-evoked ACh release from longitudinal muscle with myenteric plexus preparations between the wild type and mutant mice. Exogenously added nicotine induced contraction or relaxation of ileal circular muscle in the absence or presence of atropine, respectively, to a similar extent in both the wild type and mutant mice. These results suggest that loss of ICC-MY resulted in an impairment of the ascending and descending reflex pathways at the step before activation of cholinergic interneurons.

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Section: Introductionmentioning

confidence: 83%

Examination of the role of cholinergic myenteric neurons with the impairment of neural reflexes in the ileum of c-kit mutant mice

Okishio

Takeuchi

Fujita

et al. 2005

J. Smooth Muscle Res.

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“…Kanada et al [32] showed that a selective 5-HT 3 receptor agonist induced a dose-dependent relaxation of rat ileal circular muscle. The 5-HT 7 receptor also has an extensive vascular distribution and is responsible for the prominent, persistent vasodilator response to 5-HT in anaesthetized animals [4] .…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, studies have shown NO-mediated activity via the 5-HT 3 receptor (e.g. in the rat ileum) [32] , neuronal cell lines [33] and rat spinal cord [34] . In our study, ketan inhibited the contractile response mediated by 5-HT on the corpus cavernosum suggesting the presence of 5-HT 2A receptors which mediate smooth muscle contraction.…”

Section: Discussionmentioning

confidence: 99%

Serotonin Induces a Biphasic Response in Rabbit Cavernosal Smooth Muscle: Relevance to the Erectile Process

et al. 2007

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Introduction: Serotonin (5-hydroxytryptamine; 5-HT) can cause contraction in cavernosal smooth muscle. We further evaluated this effect of 5-HT. Methods: Organ bath studies were used. Results: 5-HT induced a sustained contraction occasionally accompanied by a transient relaxation (in 30% of rabbit cavernosal tissues) that preceded the contraction. Ondansetron and Y-25130 (both 5-HT₃ receptor antagonists) but not SB-269970 (a 5-HT₇ receptor antagonist) significantly inhibited or abolished this transient relaxation. Doxazosin (dox, an α₁-receptor antagonist) and ketanserin (ketan, a 5-HT_2A receptor antagonist) significantly inhibited or abolished the sustained contraction. The effects of dox on 5-HT-mediated contraction were concentration-dependent. Conclusions: Our findings further confirm that the peripheral serotonergic pathway may play a part in the erectile process via 5-HT_2A receptor-mediated contractile and 5-HT₃ receptor-mediated relaxant activities. Our results also support the findings of human studies, which suggest that both ketan and dox may exert beneficial effects on the erectile process.

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“…In contrast, inhibitory motor neurons of descending relaxation are variable from region to region of the intestinal tract. The role of nitric oxide in the descending relaxation was shown in the rat ileum (Kanada et al, 1992;Kanada et al, 1993), proximal colon (Hata et al, 1990a) and distal colon (Grider, 1993;Okishio et al, 2000a). Some studies also reported a role of vasoactive intestinal peptide (VIP) in the descending relaxation in the middle of distal colon (Grider and Makhlouf, 1986;Grider and Rivier, 1990;Grider, 1993) and the distal colon (Okishio et al, 2000a) of the rat.…”

Section: Introductionmentioning

confidence: 97%

Ascending contraction and descending relaxation in the distal colon of mice lacking interstitial cells of Cajal

Okishio

Takeuchi

Fujita

et al. 2005

J. Smooth Muscle Res.

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Recently an essential role of interstitial cells of Cajal (ICC) within myenteric plexus (ICC-MY) was suggested in ascending contraction and descending relaxation in the mouse ileum. The role of ICC in these neural reflexes was examined in the distal colonic segments prepared from the wild type and c-kit mutant, W/W V mice, in the present study. Localized distension of the segments from the wild type mice by using a small balloon resulted in ascending contraction and descending relaxation. In the segments from the mutant mice, localized distension also induced these neural reflexes similar to those observed in the wild type mice. Immunohistochemical examination demonstrated that ICC-MY and ICC present in muscle layers (ICC-IM) were severely disrupted in the mutant mouse, but only ICC, present within submucosal plexus (ICC-SMP), remained unchanged. In the small strips with ICC-SMP absent prepared from the mutant mouse, electrical field stimulation induced contraction or relaxation in the absence or presence of atropine, respectively. It was suggested that ICC have no important role in the ascending and descending neural reflexes in the mouse distal colon, this is in direct contrast to the role of ICC-MY in the ileum.

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Neuronal pathway involved in nitric oxide-mediated descending relaxation in rat ileum

Cited by 21 publications

References 24 publications

Examination of the role of cholinergic myenteric neurons with the impairment of neural reflexes in the ileum of c-kit mutant mice

Examination of the role of cholinergic myenteric neurons with the impairment of neural reflexes in the ileum of c-kit mutant mice

Serotonin Induces a Biphasic Response in Rabbit Cavernosal Smooth Muscle: Relevance to the Erectile Process

Ascending contraction and descending relaxation in the distal colon of mice lacking interstitial cells of Cajal

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