Neuronal mRNAs travel singly into dendrites

Batish, Mona; Bogaard, Patrick van den; Kramer, Fred Russell; Tyagi, Sanjay

doi:10.1073/pnas.1111226109

Cited by 132 publications

(131 citation statements)

References 34 publications

(56 reference statements)

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“…It was further demonstrated that endogenous MAP2, CaMKIIa, and b-actin mRNAs localize in distinct ribonucleoprotein particles which, unexpectedly, contained very few mRNA molecules (Mikl et al, 2011). Accordingly, in situ hybridization experiments with single molecule sensitivity to study 8 different and well established dendritic mRNAs showed that different mRNAs had little or no tendency to cluster together in the same granules and most likely were transported singly into dendrites (Batish et al, 2012). These recent findings suggest a simpler model in which solitary mRNAs can recruit their specific set of proteins and travel to distal dendrites, which may provide finer regulation of distinct mRNAs at individual synapses.…”

Section: Molecular Composition Of Rna Granulesmentioning

confidence: 99%

BDNF-induced local protein synthesis and synaptic plasticity

2014

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a b s t r a c tBrain-derived neurotrophic factor (BDNF) is an important regulator of synaptic transmission and longterm potentiation (LTP) in the hippocampus and in other brain regions, playing a role in the formation of certain forms of memory. The effects of BDNF in LTP are mediated by TrkB (tropomyosin-related kinase B) receptors, which are known to be coupled to the activation of the Ras/ERK, phosphatidylinositol 3-kinase/Akt and phospholipase C-g (PLC-g) pathways. The role of BDNF in LTP is best studied in the hippocampus, where the neurotrophin acts at pre-and post-synaptic levels. Recent studies have shown that BDNF regulates the transport of mRNAs along dendrites and their translation at the synapse, by modulating the initiation and elongation phases of protein synthesis, and by acting on specific miRNAs. Furthermore, the effect of BDNF on transcription regulation may further contribute to long-term changes in the synaptic proteome. In this review we discuss the recent progress in understanding the mechanisms contributing to the short-and long-term regulation of the synaptic proteome by BDNF, and the role in synaptic plasticity, which is likely to influence learning and memory formation.This article is part of the Special Issue entitled 'BDNF Regulation of Synaptic Structure, Function, and Plasticity'.

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Section: Molecular Composition Of Rna Granulesmentioning

confidence: 99%

BDNF-induced local protein synthesis and synaptic plasticity

2014

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“…98,99 In contrast, recent quantitative imaging approaches in neurons indicates that some mRNA species can transit in granules or "particles" harboring just a single mRNA molecule. [100][101][102] Why such variation in mRNP content occurs is unclear, but presumably reflects a differential requirement for the specificity of localizing individual mRNPs to particular cellular compartments under various conditions.…”

Section: Role Of Mrnp Granules In Mrna Localizationmentioning

confidence: 99%

mRNP granules

2014

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“…Development 139 (18) RNA molecules, suggesting that mRNAs are transported independently of each other in these systems (Mikl et al, 2011;Amrute-Nayak and Bullock, 2012;Batish et al, 2012).…”

Section: Reviewmentioning

confidence: 99%

“…Dissection followed by microarray analysis (Suzuki et al, 2007) Rat CA1 hippocampal neurons Stratum radiatum and lacunosum moleculare Dissection followed by deep sequencing (Cajigas et al, 2012) REVIEW Development 139 (18) RNA molecules, suggesting that mRNAs are transported independently of each other in these systems (Mikl et al, 2011;Amrute-Nayak and Bullock, 2012;Batish et al, 2012).…”

Section: Reviewmentioning

confidence: 99%

Principles and roles of mRNA localization in animal development

2012

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SummaryIntracellular targeting of mRNAs has long been recognized as a means to produce proteins locally, but has only recently emerged as a prevalent mechanism used by a wide variety of polarized cell types. Localization of mRNA molecules within the cytoplasm provides a basis for cell polarization, thus underlying developmental processes such as asymmetric cell division, cell migration, neuronal maturation and embryonic patterning. In this review, we describe and discuss recent advances in our understanding of both the regulation and functions of RNA localization during animal development.Key words: RNA localization, RNA transport, Local translation, Cell polarity, Post-transcriptional gene regulation Introduction Establishment of cell polarity is crucial for the execution of developmental programmes governing key processes, including specification of cell fates, individual or collective cell movements and specialization of somatic cell types. Cell polarization depends on the asymmetric segregation of organelles and various molecules within the cell. Polarized accumulation of RNA molecules was first visualized nearly 30 years ago, when -actin mRNA was found to be asymmetrically localized within ascidian eggs and embryos (Jeffery et al., 1983). Following this, the discovery of the first localized maternal mRNAs in Xenopus (Rebagliati et al., 1985) and Drosophila oocytes (Frigerio et al., 1986;Berleth et al., 1988) provided evidence for the earlier proposal that localized RNA determinants could be responsible for early embryonic patterning (Kandler-Singer and Kalthoff, 1976). mRNAs were soon found to be asymmetrically distributed within differentiated somatic cells, such as fibroblasts (Lawrence and Singer, 1986), oligodendrocytes (Trapp et al., 1987) and neurons (Garner et al., 1988), and to colocalize with their encoded proteins, establishing intracellular transport of mRNAs as a potential mechanism used to target the production of selected proteins to discrete sites.Significant improvements in RNA detection methods led to the identification of a growing number of localized mRNAs. Still, in the early 2000s, the set of described targeted mRNAs was limited to ~100 (reviewed by Bashirullah et al., 1998;Palacios and St Johnston, 2001) and the process of mRNA localization was thought to be restricted to specific cell types. However, recent genome-wide analyses (see Table 1) have changed this view dramatically, and strongly suggest that subcellular targeting of mRNAs is a prevalent mechanism used by polarized cells to establish functionally distinct compartments (Fig. 1). Particularly striking was the discovery that >70% of the 2314 expressed transcripts analysed in a highresolution in situ hybridization screen were subcellularly localized in Drosophila embryos (Lécuyer et al., 2007). Moreover, hundreds to thousands of mRNAs have been detected in cellular compartments as diverse as the mitotic apparatus (Blower et al., 2007;Sharp et al., 2011), pseudopodia (Mili et al., 2008), dendrites (Moccia et al., 2003;Poon et a...

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Neuronal mRNAs travel singly into dendrites

Cited by 132 publications

References 34 publications

BDNF-induced local protein synthesis and synaptic plasticity

BDNF-induced local protein synthesis and synaptic plasticity

mRNP granules

Principles and roles of mRNA localization in animal development

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