Neuronal morphology and projection analysis by multispectral tracing in densely labeled mouse brain

Roossien, Douglas H.; Webb, John; Sadis, Benjamin V; Yan, Yan; Min, Lia; Dizaji, Aslan S.; Bogart, Luke J; Mazuski, Cristina; Huth, Robert S.; Stecher, Johanna S.; Akula, Sriakhila; Lichtman, Jeff W.; Hensch, Takao K.; Herzog, Erik D.; Cai, Dawen

doi:10.1101/230458

Cited by 1 publication

(1 citation statement)

References 26 publications

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“…In another example, we may combine our techniques with the Brainbow tools, which randomly generates many spectrally distinct colors to label neighboring neurons [79,80]. Using proper sample preparation, imaging, and neuronal tracing methods, Brainbow tools permit us to trace densely labeled neurons and reconstruct neural circuits [81][82][83][84]. This combination therefore allows for the investigation of local interactions between recorded and surrounding neurons through detailed anatomical mapping.…”

Section: Discussionmentioning

confidence: 99%

High density carbon fiber arrays for chronic electrophysiology, fast scan cyclic voltammetry, and correlative anatomy

Patel

Popov²,

Caldwell³

et al. 2020

J. Neural Eng.

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Objective. Multimodal measurements at the neuronal level allow for detailed insight into local circuit function. However, most behavioral studies focus on one or two modalities and are generally limited by the available technology. Approach. Here, we show a combined approach of electrophysiology recordings, chemical sensing, and histological localization of the electrode tips within tissue. The key enabling technology is the underlying use of carbon fiber electrodes, which are small, electrically conductive, and sensitive to dopamine. The carbon fibers were functionalized by coating with Parylene C, a thin insulator with a high dielectric constant, coupled with selective re-exposure of the carbon surface using laser ablation. Main results. We demonstrate the use of this technology by implanting 16 channel arrays in the rat nucleus accumbens. Chronic electrophysiology and dopamine signals were detected 1 month post implant. Additionally, electrodes were left in the tissue, sliced in place during histology, and showed minimal tissue damage. Significance. Our results validate our new technology and methods, which will enable a more comprehensive circuit level understanding of the brain.

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