Neuronal maturation and laminar formation in the chicken optic tectum are accompanied by the transition of phosphorylated cofilin from cytoplasm to nucleus

Zhang, Wei; Chai, Xuejun; Zhang, Qi; Xie, Jiongfang; Chen, Shulin; Zhao, Shanting

doi:10.1016/j.gep.2014.09.005

Cited by 6 publications

(2 citation statements)

References 63 publications

(68 reference statements)

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“…Our recent report also agrees that phosphorylatable, wild-type CFL-1 can be detected in cell nuclei by the tetracycline inducible gene over-expression system [25]. Of interest, a recent report showed that phosphorylated cofilin can transit from the cytoplasm to the nucleus in the laminar formation of the chicken optic tectum, indicating that phospho-cofilin plays a role in neural development biology [59]. On the other hand, it has been proposed that CFL-1 is phosphorylated in the nucleus by nuclear LIMK1 and LIMK2 [60,61].…”

Section: The Actin Depolymerizing Factor (Adf)/cofilin Signaling Psupporting

confidence: 76%

The Actin Depolymerizing Factor (ADF)/Cofilin Signaling Pathway and DNA Damage Responses in Cancer

Chang¹,

Leu

Lee³

2015

IJMS

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The actin depolymerizing factor (ADF)/cofilin protein family is essential for actin dynamics, cell division, chemotaxis and tumor metastasis. Cofilin-1 (CFL-1) is a primary non-muscle isoform of the ADF/cofilin protein family accelerating the actin filamental turnover in vitro and in vivo. In response to environmental stimulation, CFL-1 enters the nucleus to regulate the actin dynamics. Although the purpose of this cytoplasm-nucleus transition remains unclear, it is speculated that the interaction between CFL-1 and DNA may influence various biological responses, including DNA damage repair. In this review, we will discuss the possible involvement of CFL-1 in DNA damage responses (DDR) induced by ionizing radiation (IR), and the implications for cancer radiotherapy.

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Section: The Actin Depolymerizing Factor (Adf)/cofilin Signaling Psupporting

confidence: 76%

The Actin Depolymerizing Factor (ADF)/Cofilin Signaling Pathway and DNA Damage Responses in Cancer

Chang¹,

Leu

Lee³

2015

IJMS

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“…In mammals, cofilin exists as two variants in addition to ADF: the non-muscle cofilin-1, and in muscle tissue, cofilin-2 [ 45 ]. Chicken tissues also contain an ADF variant, although only one cofilin form is expressed, which is closer to mammalian cofilin-2, but shows 80% identity to cofilin-1 [ 46 , 47 ]. The biological activities of mammalian and avian cofilin are, however, fully comparable [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

VEGF Triggers the Activation of Cofilin and the Arp2/3 Complex within the Growth Cone

Schlau

Terheyden-Keighley

Theis

et al. 2018

IJMS

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A crucial neuronal structure for the development and regeneration of neuronal networks is the axonal growth cone. Affected by different guidance cues, it grows in a predetermined direction to reach its final destination. One of those cues is the vascular endothelial growth factor (VEGF), which was identified as a positive effector for growth cone movement. These positive effects are mainly mediated by a reorganization of the actin network. This study shows that VEGF triggers a tight colocalization of cofilin and the Arp2/3 complex to the actin cytoskeleton within chicken dorsal root ganglia (DRG). Live cell imaging after microinjection of GFP (green fluorescent protein)-cofilin and RFP (red fluorescent protein)-LifeAct revealed that both labeled proteins rapidly redistributed within growth cones, and showed a congruent distribution pattern after VEGF supplementation. Disruption of signaling upstream of cofilin via blocking LIM-kinase (LIMK) activity resulted in growth cones displaying regressive growth behavior. Microinjection of GFP-p16b (a subunit of the Arp2/3 complex) and RFP-LifeAct revealed that both proteins redistributed into lamellipodia of the growth cone within minutes after VEGF stimulation. Disruption of the signaling to the Arp2/3 complex in the presence of VEGF by inhibition of N-WASP (neuronal Wiskott–Aldrich–Scott protein) caused retraction of growth cones. Hence, cofilin and the Arp2/3 complex appear to be downstream effector proteins of VEGF signaling to the actin cytoskeleton of DRG growth cones. Our data suggest that VEGF simultaneously affects different pathways for signaling to the actin cytoskeleton, since activation of cofilin occurs via inhibition of LIMK, whereas activation of Arp2/3 is achieved by stimulation of N-WASP.

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Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development

Yang

et al. 2017

J Mol Neurosci

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During nervous system development, neurons project axons over long distances to reach the appropriate targets for correct neural circuit formation. Sonic hedgehog (Shh) is a secreted protein and plays a key role in regulating vertebrate embryogenesis, especially in central nervous system (CNS) patterning, including neuronal migration and axonal projection in the brain and spinal cord. In the developing ventral midbrain, Shh is sufficient to specify a striped pattern of cell fates. Little is known about the molecular mechanisms underlying the Shh regulation of the neural precursor cell fate during the optic tectum development. Here, we aimed at studying how Shh might regulate chicken optic tectum patterning. In the present study, in ovo electroporation methods were employed to achieve the overexpression of Shh in the optic tectum during chicken embryo development. Besides, the study combined in ovo electroporation and neuron isolation culturing to study the function of Shh in vivo and in vitro. The fluorescent immunohistochemistry methods were used to check the related indicators. The results showed that Shh overexpression caused 87.8% of cells to be distributed to the stratum griseum central (SGC) layer, while only 39.3% of the GFP-transfected cells resided in the SGC layer in the control group. Shh overexpression also reduced the axon length in vivo and in vitro. In conclusion, we provide evidence that Shh regulates the neural precursor cell fate during chicken optic tectum development. Shh overexpression impairs neuronal migration and may affect the fate determination of transfected neurons.

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Neuronal maturation and laminar formation in the chicken optic tectum are accompanied by the transition of phosphorylated cofilin from cytoplasm to nucleus

Cited by 6 publications

References 63 publications

The Actin Depolymerizing Factor (ADF)/Cofilin Signaling Pathway and DNA Damage Responses in Cancer

The Actin Depolymerizing Factor (ADF)/Cofilin Signaling Pathway and DNA Damage Responses in Cancer

VEGF Triggers the Activation of Cofilin and the Arp2/3 Complex within the Growth Cone

Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development

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