Neuronal differentiation of human neuroblastoma SH-SY5Y cells by gangliosides

Lee, Min-Cheol; Kim, Bong-Woon; Kim, Jongsoon; Lee, Ji-Shin; Kim, Kyung-Soo; Lee, Jae-Hyuk; Nam, Jong Hee; Rowe, Sung-Man; Kim, Seung-U.

doi:10.1007/bf02478862

Cited by 5 publications

(7 citation statements)

References 37 publications

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“…A major difference between the CM-hDPSCexposed and BDNF-exposed cells was the appearance of a different subset of electron-dense intracellular vesicles and the observed release of granular material in the intercellular cleft. With the exception of the latter observation, these findings are in agreement with the scarce data available on the ultrastructure of differentiated SH-SY5Y cells (Lee et al 1997).…”

Section: Discussionsupporting

confidence: 91%

Paracrine Maturation and Migration of SH-SY5Y Cells by Dental Pulp Stem Cells

et al. 2017

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Neurological disorders are characterized by neurodegeneration and/or loss of neuronal function, which cannot be adequately repaired by the host. Therefore, there is need for novel treatment options such as cell-based therapies that aim to salvage or reconstitute the lost tissue or that stimulate host repair. The present study aimed to evaluate the paracrine effects of human dental pulp stem cells (hDPSCs) on the migration and neural maturation of human SH-SY5Y neuroblastoma cells. The hDPSC secretome had a significant chemoattractive effect on SH-SY5Y cells as shown by a transwell assay. To evaluate neural maturation, SH-SY5Y cells were first induced toward neuronal cells, after which they were exposed to the hDPSC secretome. In addition, SH-SY5Y cells subjected to the hDPSC secretome showed increased neuritogenesis compared with nonexposed cells. Maturated cells were shown to increase immune reactivity for neuronal markers compared with controls. Ultrastructurally, retinoic acid (RA) signaling and subsequent exposure to the hDPSC secretome induced a gradual rise in metabolic activity and neuronal features such as multivesicular bodies and cytoskeletal elements associated with cellular communication. In addition, electrophysiological recordings of differentiating cells demonstrated a transition toward a neuronal electrophysiological profile based on the maximum tetrodotoxin (TTX)-sensitive, Na current. Moreover, conditioned medium (CM)-hDPSC-maturated SH-SY5Y cells developed distinct features including, Cd-sensitive currents, which suggests that CM-hDPSC-maturated SH-SY5Y acquired voltage-gated Ca channels. The results reported in this study demonstrate the potential of hDPSCs to support differentiation and recruitment of cells with neuronal precursor characteristics in a paracrine manner. Moreover, this in vitro experimental design showed that the widely used SH-SY5Y cell line can improve and simplify the preclinical in vitro research on the molecular mechanisms of stem cell-mediated neuronal regeneration.

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Section: Discussionsupporting

confidence: 91%

Paracrine Maturation and Migration of SH-SY5Y Cells by Dental Pulp Stem Cells

et al. 2017

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“…There have been many reports indicating that gangliosides can induce neuronal differentiation. A ganglioside mixture extracted from bovine brain stimulated neurite outgrowth and neuronal differentiation of the SH-SY5Y cultured human neuroblastoma cell line [ 1 , 2 ]. SH-SY5Y cells differentiate into adrenergic, cholinergic, or dopaminergic neurons under stimulation by various differentiation-inducing factors such as retinoic acid, phorbol ester (12- O -tetradecanoylphorbol-13-acetate), platelet-derived growth factor (PDGF), brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), basic fibroblast growth factor, insulin-like growth factor (IGF), and dibutyryl cyclic adenosine monophosphate [ 3 , 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Structure-Activity Relationship Study of the Neuritogenic Potential of the Glycan of Starfish Ganglioside LLG-3 ‡

et al. 2015

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LLG-3 is a ganglioside isolated from the starfish Linchia laevigata. To clarify the structure-activity relationship of the glycan of LLG-3 toward rat pheochromocytoma PC12 cells in the presence of nerve growth factor, a series of mono- to tetrasaccharide glycan derivatives were chemically synthesized and evaluated in vitro. The methyl group at C8 of the terminal sialic acid residue was crucial for neuritogenic activity, and the terminal trisaccharide moiety was the minimum active motif. Furthermore, the trisaccharide also stimulated neuritogenesis in human neuroblastoma SH-SY5Y cells via mitogen-activated protein kinase (MAPK) signaling. Phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was rapidly induced by adding 1 or 10 nM of the trisaccharide. The ratio of phosphorylated ERK to ERK reached a maximum 5 min after stimulation, and then decreased gradually. However, the trisaccharide did not induce significant Akt phosphorylation. These effects were abolished by pretreatment with the MAPK inhibitor U0126, which inhibits enzymes MEK1 and MEK2. In addition, U0126 inhibited the phosphorylation of ERK 1/2 in response to the trisaccharide dose-dependently. Therefore, we concluded that the trisaccharide promotes neurite extension in SH-SY5Y cells via MAPK/ERK signaling, not Akt signaling.

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“…Der Alltag sieht häufig komplizierter aus. Lee et al haben in einer Publikation aufgezeigt, dass bei 472 befragten Pflegekräften 7,8 Prozent eine Plantarfasziitis (PFS) auftrat und 7,0 Prozent unter einem Hallux valgus (HV) litten 3 . In der Veröffentlichung von Stolt et al zeigt sich, dass Probleme bei der richtigen Schuhauswahl das OP-Personal betrafen.…”

Section: Risiken Und Einflussfaktorenunclassified

“…Lee et al zeigten in ihrer Publikation die häufigste Fußkrankheit auf. Hier wurden HV als auch PFS als gesundheitliche Problemregionen beschrieben 3 4 . Die Schmerzintensität ist sowohl für die PFS als auch für HV sehr hoch.…”

Section: Diskussionunclassified