Neuron stem cell NLRP6 sustains hippocampal neurogenesis to resist stress-induced depression

Gut microbiota-derived short-chain fatty acids ameliorate methamphetamine-induced depression- and anxiety-like behaviors in a Sigmar-1 receptor-dependent manner

“…Finally, the sections were incubated with the donkey anti-rabbit IgG H&L secondary antibody (1:500, Life technologies, Cat#: A21207) for 1 h at room temperature. The nucleus was stained with DAPI (Sigma–Aldrich) 46 . Fluorescence was captured using a fluorescence microscope (Leica M165FC, Leica Microsystems, Nussloch, Germany), and the number of positive cells per visual field was counted in a blind manner.…”

Recent advances in anxiety disorders: Focus on animal models and pathological mechanisms

“…In proapoptotic gene Bax‐knockout mice, increased adult neurogenesis not only reduced anxiety‐ and depression‐related behaviors in mice administered GCs for a long time but also counteracted the effects of unpredictable chronic mild stress and endocrine levels 109 . Stress and anxiety also decrease hippocampal neuron numbers, and low neurogenesis fails to mitigate stress and can further exacerbate anxiety 110 . Furthermore, material and branch points on apical dendrites are significantly reduced in the hippocampus and PFC in animal models of anxiety, especially in the high‐anxiety group 111 .…”

“… 109 Stress and anxiety also decrease hippocampal neuron numbers, and low neurogenesis fails to mitigate stress and can further exacerbate anxiety. 110 Furthermore, material and branch points on apical dendrites are significantly reduced in the hippocampus and PFC in animal models of anxiety, especially in the high‐anxiety group. 111 Loss of dendrites contributes to animals being more sensitive to stress and more prone to anxiety.…”

Nlrp6 protects from corticosterone-induced NSPC ferroptosis by modulating RIG-1/MAVS-mediated mitophagy

“…More interesting, gastrointestinal Nlrp6 expression is inhibited by corticotropin-releasing hormone in mice with water-avoidance stress [ 23 ]. In our recent study, Nlrp6 is highly expressed in hippocampal NSPCs, and down-regulated in response to chronic stress, as well as the major stress hormone, corticosterone [ 24 ]. Knockout of Nlrp6 causes mitochondrial dysfunction and proliferation disorders in NSPCs, resulting in hippocampal neurogenesis decline in mice [ 24 ].…”

“…In our recent study, Nlrp6 is highly expressed in hippocampal NSPCs, and down-regulated in response to chronic stress, as well as the major stress hormone, corticosterone [ 24 ]. Knockout of Nlrp6 causes mitochondrial dysfunction and proliferation disorders in NSPCs, resulting in hippocampal neurogenesis decline in mice [ 24 ]. This study strongly suggests that Nlrp6 may participate in the defense of NSPCs against external stimuli, however, the underlying mechanisms need to be further investigated.…”