Neuron-specific expression of neuroglobin in mammals

Laufs, Tilmann; Wystub, Sylvia; Reuss, Stefan; Burmester, Thorsten; Saaler-Reinhardt, Sigrid; Hankeln, Thomas

doi:10.1016/j.neulet.2004.02.072

Cited by 56 publications

(39 citation statements)

References 22 publications

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“…This quantitative difference can be interpreted in terms of an O 2 supply function and/or an ROS detoxification role of Ngb as an adaptive strategy to alleviate hypoxic stress. In Spalax, the interpretation is more complicated: we show that Ngb in the mole rat is localized in neurons and astrocytes, whereas in the brain of hypoxia-sensitive rodents like mouse or rat, Ngb is localized primarily in neurons (19,39,45). Thus, both cell types contribute to the elevated Ngb expression level in Spalax brain, and we are currently unable to separately quantify neuronal and glial expression.…”

Section: Increased Globin Levels In Spalax Indicate a Function In Hypmentioning

confidence: 90%

Neuroglobin, cytoglobin, and myoglobin contribute to hypoxia adaptation of the subterranean mole rat Spalax

Avivi

Gerlach²,

Joel³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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122

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The subterranean mole rat Spalax is an excellent model for studying adaptation of a mammal toward chronic environmental hypoxia. Neuroglobin (Ngb) and cytoglobin (Cygb) are O 2 -binding respiratory proteins and thus candidates for being involved in molecular hypoxia adaptations of Spalax. Ngb is expressed primarily in vertebrate nerves, whereas Cygb is found in extracellular matrix-producing cells and in some neurons. The physiological functions of both proteins are not fully understood but discussed with regard to O 2 supply, the detoxification of reactive oxygen or nitrogen species, and apoptosis protection. Spalax Ngb and Cygb coding sequences are strongly conserved. However, mRNA and protein levels of Ngb in Spalax brain are 3-fold higher than in Rattus norvegicus under normoxia. Importantly, Spalax expresses Ngb in neurons and additionally in glia, whereas in hypoxia-sensitive rodents Ngb expression is limited to neurons. Hypoxia causes an approximately 2-fold downregulation of Ngb mRNA in brain of rat and mole rat. A parallel regulatory response was found for myoglobin (Mb) in Spalax and rat muscle, suggesting similar functions of Mb and Ngb. Cygb also revealed an augmented normoxic expression in Spalax vs. rat brain, but not in heart or liver, indicating distinct tissue-specific functions. Hypoxia induced Cygb transcription in heart and liver of both mammals, with the most prominent mRNA up-regulation (12-fold) in Spalax heart. Our data suggest that tissue globins contribute to the remarkable tolerance of Spalax toward environmental hypoxia. This is consistent with the proposed cytoprotective effect of Ngb and Cygb under pathological hypoxic/ischemic conditions in mammals.

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Section: Increased Globin Levels In Spalax Indicate a Function In Hypmentioning

confidence: 90%

Neuroglobin, cytoglobin, and myoglobin contribute to hypoxia adaptation of the subterranean mole rat Spalax

Avivi

Gerlach²,

Joel³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

122

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“…Thus, the remaining cell lines represented a loss of NRSF/REST expression with neuronal transcripts increased (Coulson et al, 2000;Gurrola-Diaz et al, 2003;Neumann et al, 2004). For example, expression levels of NCAM, synapsin 1 and, in particular, neuroglobin (Laufs et al, 2004) showed a strict inverse correlation with NRSF/REST expression, or correlated with the co-expression of sNRSF. Transcripts of glycine receptor alpha 1 subunit were detected in only two of the cell lines analysed, whereas transcription of L1-CAM and synaptophysin was less Modulation of small-cell lung cancer phenotype by NRSF/REST A Kreisler et al restrictive.…”

Section: Discussionmentioning

confidence: 99%

Regulation of the NRSF/REST gene by methylation and CREB affects the cellular phenotype of small-cell lung cancer

et al. 2010

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“…Cells from the human neuroblastoma cell lines SH-SY5Y were cultured in Dulbecco's modified Eagle's medium (Clontech, USA) supplemented with 10% fetal bovine serum (Invitrogen, USA), Lglutamine and antibiotics, and incubated in a humidified atmosphere of 5% CO 2 at 37 ° C. Ngb could be detected in SH-SY5Y cell lines [22] . For exposure to hypoxia, cell culture plates were incubated in a modular incubator chamber and flushed with a gas mixture containing 1% O 2 , and 5% CO 2 .…”

Section: Cell Culture and Exposure To Hypoxiamentioning

confidence: 99%

Antihypoxic Effects of Neuroglobin in Hypoxia-Preconditioned Mice and SH-SY5Y Cells

Shao

Gong²,

Li³

et al. 2009

Neurosignals

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This work aims at investigating the neuroprotective effects of neuroglobin (Ngb) in vivo and in vitro. RT-PCR and Western blotting were used to examine Ngb mRNA and protein levels in the mouse cortex after acute and repeated exposure to hypoxia. The cDNAs of mouse Ngb were cloned and transfected into SH-SY5Y cells to examine Ngb function in vitro. Expression of Ngb and mRNA was upregulated in the cortex of mice preconditioned by repetitive exposure to hypoxia. Tolerance to hypoxia of Ngb-transformed SH-SY5Y cells was enhanced. These results suggest that Ngb might be involved in hypoxic preconditioning which protects neurons from hypoxic injury.

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Neuron-specific expression of neuroglobin in mammals

Cited by 56 publications

References 22 publications

Neuroglobin, cytoglobin, and myoglobin contribute to hypoxia adaptation of the subterranean mole rat Spalax

Neuroglobin, cytoglobin, and myoglobin contribute to hypoxia adaptation of the subterranean mole rat Spalax

Regulation of the NRSF/REST gene by methylation and CREB affects the cellular phenotype of small-cell lung cancer

Antihypoxic Effects of Neuroglobin in Hypoxia-Preconditioned Mice and SH-SY5Y Cells

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