Neuron-specific enolase (NSE) as a neuroendocrine cell marker in the human fetal pancreas

Dorsche, Herwig Hahn von; Fält, K; Hahn, Hans-Jürgen; Reiher, H

doi:10.1016/s0065-1281(89)80073-x

Cited by 10 publications

(6 citation statements)

References 3 publications

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“…It is known that the endocrine cells of pancreatic islets are similar to nervous cells in a number of biochemical and physiological characteristics. Pancreatic islet cells share similarities with neurons in expressing neural markers, including GFAP, glutamic acid decarboxylase (GAD), tyrosine hydroxylase (TH), neuropeptide Y (NPY), NSE, neural cell adhesion molecules (NCAM), the beta-III tubulin, the synaptophysin, the nerve growth factors and their receptors, chromogranin A, and others (12, 19, 38–43). Alpha-cells contain acetylcholine (44).…”

Section: Discussionmentioning

confidence: 99%

Ontogeny of Neuro-Insular Complexes and Islets Innervation in the Human Pancreas

et al. 2014

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The ontogeny of the neuro-insular complexes (NIC) and the islets innervation in human pancreas has not been studied in detail. Our aim was to describe the developmental dynamics and distribution of the nervous system structures in the endocrine part of human pancreas. We used double-staining with antibodies specific to pan-neural markers [neuron-specific enolase (NSE) and S100 protein] and to hormones of pancreatic endocrine cells. NSE and S100-positive nerves and ganglia were identified in the human fetal pancreas from gestation week (gw) 10 onward. Later the density of S100 and NSE-positive fibers increased. In adults, this network was sparse. The islets innervation started to form from gw 14. NSE-containing endocrine cells were identified from gw 12 onward. Additionally, S100-positive cells were detected both in the periphery and within some of the islets starting at gw 14. The analysis of islets innervation has shown that the fetal pancreas contained NIC and the number of these complexes was reduced in adults. The highest density of NIC is detected during middle and late fetal periods, when the mosaic islets, typical for adults, form. The close integration between the developing pancreatic islets and the nervous system structures may play an important role not only in the hormone secretion, but also in the islets morphogenesis.

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Section: Discussionmentioning

confidence: 99%

Ontogeny of Neuro-Insular Complexes and Islets Innervation in the Human Pancreas

et al. 2014

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“…Many markers have gradually been found to be expressed in a large number of prostatic NE cancer cases227, such as K18 and K8 cytokeratins 228, α-methylacyl-CoA-racemase (AMACR) 228, plasma anterior gradient 2 (AGR2) 229, and PGP9.5 230. Additionally, neuron-specific enolase (NSE) and chromogranin A (CgA) may be the most frequently expressed neuropeptides 231-233, but they also have limitations in sensitivity, specificity, reproducibility, etc. 234.…”

Section: Neuroendocrine Cellmentioning

confidence: 99%

Role of tumor microenvironment in tumorigenesis

et al. 2017

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Tumorigenesis is a complex and dynamic process, consisting of three stages: initiation, progression, and metastasis. Tumors are encircled by extracellular matrix (ECM) and stromal cells, and the physiological state of the tumor microenvironment (TME) is closely connected to every step of tumorigenesis. Evidence suggests that the vital components of the TME are fibroblasts and myofibroblasts, neuroendocrine cells, adipose cells, immune and inflammatory cells, the blood and lymphatic vascular networks, and ECM. This manuscript, based on the current studies of the TME, offers a more comprehensive overview of the primary functions of each component of the TME in cancer initiation, progression, and invasion. The manuscript also includes primary therapeutic targeting markers for each player, which may be helpful in treating tumors.

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“…detected immunOfluOrescence, immunOcytOchemis-Although insulin is present in the human fetal pancreas try, and electron microscopy (1)(2)(3)(4). from 10 wk gestation, insulin release remains practically Studies in systems, including the pan-unresponsive to glucose stimulation until birth (6).…”

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confidence: 99%

Developmental Gene Expression in the Human Fetal Pancreas

et al. 1994

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Differential developmental regulation of pancreasspecific genes has not been reported for the human fetal pancreas. We have therefore undertaken a systematic, quantitative analysis of the transcriptional levels of various genes in the human pancreas at different stages of fetal and postnatal development. Using sensitive ribonuclease protection assays, in situ hybridization, and the polymerase chain reaction, our results indicate the following: 1 ) Transcriptional levels of insulin and amylin remain lower in the fetal than in the adult pancreas, whereas glucagon and somatostatin mRNA levels are consistently greater after 14 wk gestation than postnatally. These results are in agreement with previous immunohistochemical studies of these gene products.2 ) The reg gene exhibits a 20-fold increase in mRNA levels after 16 wk gestation. The gene is expressed exclusively in the acinar cells and does not colocalize with insulin. This restricted exocrine expression does not indicate a direct role for the reg gene in islet development. 3 ) Glucose transporter 2 and glucokinase mRNA are detectable as early as 13 wk gestation and remain low throughout development. Glucose transporter 1 reaches adult transcriptional levels by 18 wk gestation. The early detection of glucose transporter 2 and glucokinase implies that lack of expression of these "glucose sensor" genes does not account for the known insensitivity of the fetal p-cells to glucose. (Pediafr Res 36: 537-544, 1994)

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Neuron-specific enolase (NSE) as a neuroendocrine cell marker in the human fetal pancreas

Cited by 10 publications

References 3 publications

Ontogeny of Neuro-Insular Complexes and Islets Innervation in the Human Pancreas

Ontogeny of Neuro-Insular Complexes and Islets Innervation in the Human Pancreas

Role of tumor microenvironment in tumorigenesis

Developmental Gene Expression in the Human Fetal Pancreas

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