Neuron differentiation in hydra involves dividing intermediates

Bode, Hans R.; Gee, Lydia; Chow, Margaret

doi:10.1016/0012-1606(90)90292-q

Cited by 30 publications

(24 citation statements)

References 40 publications

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“…The total number of interstitial cells in these lower peduncles varied from 50 to 500 depending on the condition of the hydra culture. Such fluctuations in the animals have also been reported by Bode et al (1990). All the experiments reported here involved animals with cell numbers as shown in Table 1.…”

Section: Cellular Composition Of the Pedunclesupporting

confidence: 80%

“…The cellular composition of the peduncle is different from the gastric tissue: it has a high concentration of nerve cells, reduced numbers of interstitial cells, and no differentiating nematocytes (Bode et al, 1973). Many of the interstitial cells in this tissue exhibit the morphology of nerve cell precursors (Bode et al, 1990). …”

Section: Cellular Composition Of the Pedunclementioning

confidence: 98%

“…Recently Heimfeld and Bode (1984) and Bode et al (1990) have suggested that the nerve cell differentiation pathway contains cycling intermediates. Evidence for this hypothesis was based on the observation that some cells having the morphology of typical nerve precursors (Heimfeld and Bode, 1984; could be labeled with [3H]thymidine.…”

Section: Introductionmentioning

confidence: 99%

“…The peduncle of the stalked hydra Hydra oligactis is well suited for such experiments since it is a site of active nerve cell differentiation (Bode et al, 1990) and since it contains higher numbers of interstitial cells, which are putative nerve cell precursors, than other species. In the present experiments we have determined the differentiation and proliferation capacity of interstitial cells in peduncles of Hydra oligactis both in situ and by explanting them from the peduncle into a gastric environment which can support stem cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

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Putative intermediates in the nerve cell differentiation pathway in hydra have properties of multipotent stem cells

Holstein¹,

David²

1990

Developmental Biology

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We have investigated the properties of nerve cell precursors in hydra by analyzing the differentiation and proliferation capacity of interstitial cells in the peduncle of Hydra oligactis, which is a region of active nerve cell differentiation.Our results indicate that about 50% of the interstitial cells in the peduncle can grow rapidly and also give rise to nematocyte precursors when transplanted into a gastric environment. If these cells were committed nerve cell precursors, one would not expect them to differentiate into nematocytes nor to proliferate apparently without limit. Therefore we conclude that cycling interstitial cells in peduncles are not intermediates in the nerve cell differentiation pathway but are stem cells. The remaining interstitial cells in the peduncle are in G1 and have the properties of committed nerve cell precursors . Thus, the interstitial cell population in the peduncle contains both stem cells and noncycling nerve precursors. The presence of stem cells in this region makes it likely that these cells are the immediate targets of signals which give rise to nerve cells.

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Section: Cellular Composition Of the Pedunclesupporting

confidence: 80%

Section: Cellular Composition Of the Pedunclementioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Putative intermediates in the nerve cell differentiation pathway in hydra have properties of multipotent stem cells

Holstein¹,

David²

1990

Developmental Biology

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“…Table 1 shows the quantitative distribution of cell types in elutriated fractions: Fraction 1 contains 86% of all nerve cells. There is also a significant number of small interstitial cells, which could be either nerve cell precursors or early S-phase interstitial cells (Holstein and David 1990b;Bode et al 1990). Fraction 1 also contains a small number of differentiation and/or mature nematocytes (5%).…”

Section: Separation Of Hydra Cells By Counterflow Centrifugation Elutmentioning

confidence: 99%

A quantitative method for separation of livingHydra cells

Greber¹,

David²,

Holstein³

1992

Roux's Arch Dev Biol

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Summary. We describe a rapid method for the isolation of large numbers of living Hydra cells of defined cell type in an isotonic cell medium (Gierer et al. 1972). Intact animals are enzymatically dissociated into a single cell suspension and the various cell types separated in less than one hour by counterflow centrifugation elutriation. Cell loss is minimal. RNA isolated from various fractions can be probed with cell type specific cDNAclones.

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Structure, development, and maintenance of the nerve net of the body column in hydra

1996

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The anatomy and developmental dynamics of the nerve net in the body column of Hydra viridissima were examined immunocytochemically with a monoclonal antibody (CC04) that recognizes an antigen in nerve cells and with an antiserum against vasopressin. CC04+ neuron cell bodies, their neurites, and vasopressin-like-immunoreactive (VLI+) neurites could be clearly visualized on whole-mount preparations. All neurites of the CC04+ neurons in the body column were VLI+. However, only half of the VLI+ neurites in the body column were CC04+. Immunocytochemical analysis of macerated preparations showed that half of the neurons in the gastric region of the body column were CC04+. These results suggest that most of the neurons in the gastric region are VLI+. The density of the VLI+ neurites was uniform along the entire length of the body column. The CC04+ neuron density in the gastric region remained constant at all stages of asexual development and during foot regeneration. After pulse-labeling with 5-bromo-2'-deoxyuridine (BrdU), CC04+ neurons with labeled nuclei appeared in the body column. We conclude that neuron density in the gastric region is maintained at a constant value by insertion of new neurons in parallel with continuous epithelial cell division.

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Neuron differentiation in hydra involves dividing intermediates

Cited by 30 publications

References 40 publications

Putative intermediates in the nerve cell differentiation pathway in hydra have properties of multipotent stem cells

Putative intermediates in the nerve cell differentiation pathway in hydra have properties of multipotent stem cells

A quantitative method for separation of livingHydra cells

Structure, development, and maintenance of the nerve net of the body column in hydra

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