Neuron-derived exosomes with high miR-21-5p expression promoted polarization of M1 microglia in culture

Yin, Zhenyu; Han, Zhaoli; Hu, Tianpeng; Zhang, Shishuang; Ge, Xintong; Huang, Shan; Wang, Lu; Yu, Jinwen; Li, Wenzhu; Wang, Yan; Li, Dai; Zhao, Jing; Wang, Yifeng; Zuo, Yan; Liu, Ying; Kong, Xiaodong; Chen, Fanglian; Lei, Ping

doi:10.1016/j.bbi.2019.11.004

Cited by 92 publications

(51 citation statements)

References 49 publications

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“…Enhanced expression of miRNA‐19a‐3p in sEVs from α‐synuclein transgenic SH‐SY5Y cells suppressed autophagy in the recipient human microglia by regulating the PTEN/AKT/mammalian target of rapamycin (mTOR) signaling pathway, thus contributing to microglia failure in α‐synuclein degradation (T. Zhou et al, 2019). miRNA‐21‐5p was found expressed in BV2 microglia after LPS treatment (Yin et al, 2020). miRNA‐21 was demonstrated to have an anti‐inflammatory effect and to downregulate genes such as B‐cell translocation gene 2 (Btg2) that suppresses microglia proliferation (Abels et al, 2019; Sheedy, 2015).…”

Section: Exosomes and Mirnas Regulate Microglia‐induced Inflammation mentioning

confidence: 99%

Regulatory function of microRNAs in microglia

Brites

2020

Glia

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Microglia are CNS‐resident cells involved in immune surveillance and maintenance of intercellular homeostasis, while also contributing to neurologic pathologies. MicroRNAs (miRNAs) are a class of small (~22 nucleotides) single‐stranded noncoding RNAs that participate in gene regulation at the post‐transcriptional level. miRNAs typically bind to the untranslated region (3′ UTR) of RNAs. It has been shown that miRNAs are important players in controlling inflammation and that their abnormal expression is linked to cancer and ageing, and to the onset and progression of neurodegenerative disease. Furthermore, miRNAs participate in intercellular trafficking. Thus, miRNAs are released from cells in a free form, bound to proteins or packaged within extracellular vesicles (EVs), exerting paracrine and long distance signaling. In this review, recent findings on the role of miRNAs as drivers of microglia phenotypic changes and their cotribution in neurological disease are addressed.

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Section: Exosomes and Mirnas Regulate Microglia‐induced Inflammation mentioning

confidence: 99%

Regulatory function of microRNAs in microglia

Brites

2020

Glia

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“…Differences of cell sources, isolation protocols, and culture environment can affect the properties of MSCs (Table 2). In the previous research, autologous MSCs are different from those of healthy donors, which will influence the outcomes of treatment [97]. Compared with other cell types, MSCs are able to produce higher doses of exosomes.…”

Section: Msc-exos Isolation Storage and Administrationmentioning

confidence: 99%

“…The cell density and content of secreted growth factors in MSCs cultured with three-dimensional (3D) spheroid were more than those with traditional monolayer culture [98]. Unlike cell transplantation, exosomes do not need to be cultured to a suitable density before transplantation, which lays the foundation for the treatment of acute diseases [97].…”

Section: Msc-exos Isolation Storage and Administrationmentioning

confidence: 99%

“…For example, microglia plays the role of first innate immune defensive line in brain. When activated, microglia exhibit two different polarized phenotypes namely M1/M2 [97,148]. In vivo and vitro models, elevating the levels of miR-124-3p in microglial exosomes, result in the inhibition of neuronal inflammation by promoting anti-inflammatory M2 polarization and contribute to alleviating neurodegeneration [98,105].…”

Section: Immunomodulatory Effects Of Msc-exosmentioning

confidence: 99%

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Mesenchymal stem cell-derived exosome: a promising alternative in the therapy of Alzheimer’s disease

Guo

Yin

Chen³

et al. 2020

Alz Res Therapy

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Alzheimer’s disease (AD) has been a devastating public health with the development of global aging. Approaches for reducing the current AD epidemic are becoming a primary focus of human healthcare due to the lack of achieved lasting and complete remission strategies to treat AD with the characteristics of heterogeneity and complexity. Exosomes, which is the new emerging approach to intercellular communication, provide novel perspective on identified therapeutic strategies of AD. Mesenchymal stem cell-derived exosomes (MSC-exos) are emerging to be an appealing therapeutic tool for AD, with the donor-derived properties and the characteristics of minimal immunogenicity, effortless storage, nature delivery vehicles, and low risks of tumor formation based on the previous researches. In this review, we elaborate the mechanism of MSC-exos in the treatment of AD and discuss limitations in the clinical application.

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“…EVs, or specifically referred as exosomes, constituting diverse cargo like RNA, protein or lipids, capable of transmitting contents through BBB, appear to be the most effective courier in the neuroinflammation and nerve regeneration 21 , 88 , 89 . Neurons can generate exosomes enriched with miR-21-5p to regulate the MG towards to M1 type 90 . Correspondingly, glia EVs tend to modulate synaptic plasticity via miR-146a-5p 91 , even with the sphingolipid on the membrane, leading to the excitatory transmission increase in neuron 92 .…”

Section: Communication Crosstalk and Integration In Neuroinflammatiomentioning

confidence: 99%

Niche Cells Crosstalk In Neuroinflammation After Traumatic Brain Injury

Jia¹,

Wang²,

Ye³

et al. 2021

Int. J. Biol. Sci.

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Traumatic brain injury (TBI) is recognized as the disease with high morbidity and disability around world in spite of the work ongoing in neural protection. Due to heterogeneity among the patients, it's still hard to acquire satisfying achievements in clinic. Neuroinflammation, which exists since primary injury occurs, with elusive duality, appear to be of significance from recovery of injury to neurogenesis. In recent years, studied have revealed that communication in neurogenic niche is more than “cell to cell” communication, and study on NSCs represent it as central role in the progress of neural regeneration. Hence, the neuroinflammation-affecting crosstalk after TBI, and clarifying definitive role of NSCs in the course of regeneration is a promising subject for researchers, for its great potential in overcoming the frustrating status quo in clinic, promoting welfare of TBI patient.

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Neuron-derived exosomes with high miR-21-5p expression promoted polarization of M1 microglia in culture

Cited by 92 publications

References 49 publications

Regulatory function of microRNAs in microglia

Regulatory function of microRNAs in microglia

Mesenchymal stem cell-derived exosome: a promising alternative in the therapy of Alzheimer’s disease

Niche Cells Crosstalk In Neuroinflammation After Traumatic Brain Injury

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