Neuromodulatory and oxidative stress evaluations in African catfish <i>Clarias gariepinus</i> exposed to antipsychotic drug chlorpromazine

Atama, Chinedu Ifeanyi; Nnaji, Excellence Chidera; Ezeoyili, Ifeanyi Christian; Udeani, Faith Okwukwe; Onovo, Chioma Juliet; Ossai, N.I.; Aguzie, Ifeanyi Oscar

doi:10.1080/01480545.2020.1822391

Cited by 13 publications

(7 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other responses such as opercula movement and swimming were less pronounced with increasing CPZ concentrations. Similar behavioral responses were reported in Carassius auratus exposed to CPZ (Li et al, 2008), C. gariepinus exposed to chloramphenicol and CPZ (Nwani et al, 2014a;Atama et al, 2020) and Danio rerio exposed to tramadol (Bachour et al, 2020). Zang (2003) also reported behavioral abnormalities in animals after administration of small doses of CPZ.…”

Section: Discussionsupporting

confidence: 59%

“…It has also suppressed steroidogenesis in organisms (Hinfray et al, 2011;Wassmur et al, 2013). In a related report, CPZ elevated brain acetylcholinesterase values, lipid peroxidation and caused variations in the values of antioxidant enzymes in African cat sh Clarias gariepinus (Atama et al, 2020). Further, pharmaceuticals and personal care products residues in the aquatic system can cause irreversible changes in the hematological, biochemical and processes in the organisms.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hematological, biochemical, and biometric changes in Clarias gariepinus exposed to antipsychotic drug chlorpromazine

Okpe

Eze²,

Ezinwa³

et al. 2022

Environ Sci Pollut Res

View full text Add to dashboard Cite

Chlorpromazine (CPZ) is a neuroleptic and antipsychotic medication for individuals suffering from schizophrenia and other medical conditions. This study investigated the effects of CPZ on the hematological, biochemical, and biometric characteristics in juvenile Clarias gariepinus. The sh were exposed to 0.53, 1.06 and 2.11 mgl −1 CPZ for 15 days after which they were withdrawn from the toxicant and allowed to recover for 5 days. Blood were sampled from the sh on day 1, 5, 10, 15 and during the 5day recovering for hematological and biochemical analysis and thereafter, the sh were sacri ced for the morphometric analysis. While the values of the white blood cells signi cantly increased in the exposed sh, the hemoglobin, erythrocytes and packed cell volume decreased. Compared with the control, there were no signi cant differences in the values of the blood derivatives in the exposed sh. The values of protein and glucose reduced but that of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase were signi cantly elevated. Though there was no signi cant difference in the condition factor, a signi cant increase in hepatosomatic index occurred on day-15 at 5.28 mg/L CPZ.After the 5-day withdrawal from the drug, most of the studied parameters returned to the control values. The present study indicated that CPZ is toxic to sh and should be used with utmost care to guard against toxicological effect on non-target organisms.

show abstract

Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Hematological, biochemical, and biometric changes in Clarias gariepinus exposed to antipsychotic drug chlorpromazine

Okpe

Eze²,

Ezinwa³

et al. 2022

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

“…Secondly, GABA and glutamate (Glu) concentrations, as well as transcript levels associated with GABA and glutamate receptor mRNA levels (gabra1, grin1b, and gria2b), and glutamate metabolic pathways (gad1b and abat) were determined in larval fish. Moreover, since acetylcholinesterase (AChE) is a widely used enzymatic biomarker of neurotoxicity and is often measured in studies to detect neurotoxic effects of various contaminants in the aquatic environment [31,32], AChE activity was also determined.…”

Section: Introductionmentioning

confidence: 99%

Development and Molecular Investigation into the Effects of Carbamazepine Exposure in the Zebrafish (Danio rerio)

Chen

Yang

Zhao

et al. 2020

IJERPH

View full text Add to dashboard Cite

Concerns regarding environmental exposures and the impacts of pharmaceuticals on non-target aquatic organisms continue to increase. The antiepileptic drug carbamazepine (CBZ) is often detected as an aquatic contaminant and can disrupt various behaviors of fishes. However, there are few reports which investigate the mechanism of CBZ action in fish. The aim of the current study was to evaluate the effects of CBZ on embryonic development (i.e., hatching rate, heart rate, and body length) and early spontaneous movement. Moreover, we sought to investigate potential mechanisms by focusing on the gamma-aminobutyric acid (GABA) neurotransmitter system in zebrafish 6 days after of exposure. The results show that CBZ exposure did not cause significant effects on embryo development (hatching rate, heart rate, nor body length) at the test concentrations. However, the early spontaneous movement of embryos was inhibited following 10 μg/L CBZ exposure at 28–29 h post-fertilization (hpf). In addition, acetylcholinesterase (AChE) activity and GABA concentrations were increased with exposure, whereas glutamate (Glu) concentrations were decreased in larval zebrafish. Gene expression analysis revealed that GABA and glutamate metabolic pathways in zebrafish larvae were altered following exposure to CBZ. GABA transaminase (abat) and glutamic acid decarboxylase (gad1b) decreased to 100 µg/L, and glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b) as well as the glutamate receptor, ionotropic, α-amino-3hydroxy-5methylisoxazole-4propionic 2b (gria2b) were down-regulated with exposure to 1 µg/L CBZ. Our study suggests that CBZ, which can act as an agonist of the GABAA receptor in humans, can also induce alterations in the GABAergic system in fish. Overall, this study improves understanding of the neurotoxicity and behavioral toxicity of zebrafish exposed to CBZ and generates data to be used to understand mechanisms of action that may underlie antiepileptic drug exposures.

show abstract

“…The acute 24 h LC 50 obtained for CPZ (0.833–1.795 mg/L) with B. calyciflorus is higher than those obtained for aquatic organisms such as the protozoan Spirostomum ambiguum (24 h EC 50 of 0.35 mg/L), the freshwater goldfish Carassius auratus (48 h EC 50 of 0.43 mg/L) and the crustaceans Thamnocephalus platyurus (24 h LC 50 of 0.62 mg/L), similar to the D. magna (48 h EC 50 of 1.805 mg/L) [ 14 , 30 , 48 ]. Other studies on CPZ ecotoxicity are difficult to compare with our findings because the experimental endpoints differ greatly [ 49 , 50 , 51 ]. In conclusion, the sensitivity of B. calyciflorus to CPZ is comparable to that of D. magna , but more sensitive than that of fish and protozoa.…”

Section: Discussionmentioning

confidence: 66%

Inhibitory Effects of Antipsychotic Chlorpromazine on the Survival, Reproduction and Population Growth Other Than Neurotransmitters of Zooplankton in Light of Global Warming

Feng

Zhang

Gao

et al. 2022

IJERPH

View full text Add to dashboard Cite

Global warming and environmental pollution have created a unique combination of abiotic and biotic stresses to zooplankton. However, little information is available on the effects of antipsychotic drugs commonly used to treat psychosis, such as chlorpromazine (CPZ), on non-target aquatic organisms in light of global warming. This study investigated how dopamine concentrations (DAC), acute toxicity and chronic toxicity of Brachionus calyciflorus changed in response to CPZ and gradually increasing temperatures. The results showed that the concentration range of rotifer DAC was 1.06~2.51 ng/g. At 18, 25 and 32 °C, the 24 h LC50 was 1.795, 1.242 and 0.833 mg/L, respectively. Compared to the control, exposure to CPZ significantly decreased life expectancy at hatching, the net reproduction rate, generation time, population growth rate and dopamine concentration of B. calyciflorus in all three temperatures (p < 0.05). The toxicity of CPZ to rotifers was increased by high temperature. These findings indicated that CPZ is highly toxic to rotifers, displaying high ecological risks to aquatic ecosystems.

show abstract

Neuromodulatory and oxidative stress evaluations in African catfish Clarias gariepinus exposed to antipsychotic drug chlorpromazine

Cited by 13 publications

References 33 publications

Hematological, biochemical, and biometric changes in Clarias gariepinus exposed to antipsychotic drug chlorpromazine

Hematological, biochemical, and biometric changes in Clarias gariepinus exposed to antipsychotic drug chlorpromazine

Development and Molecular Investigation into the Effects of Carbamazepine Exposure in the Zebrafish (Danio rerio)

Inhibitory Effects of Antipsychotic Chlorpromazine on the Survival, Reproduction and Population Growth Other Than Neurotransmitters of Zooplankton in Light of Global Warming

Contact Info

Product

Resources

About