Neuromedin K: A novel mammalian tachykinin identified in porcine spinal cord

Kangawa, Kenji; Minamino, Naoto; Fukuda, Akinori; Matsuo, Hisayuki

doi:10.1016/0006-291x(83)90813-6

Cited by 406 publications

(79 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The hearts were sliced thinly and boiled in 100 liters of ion-exchanged water for 10 min to prevent proteolytic fragmentation (17). The boiled tissue and supernatant were cooled to room temperature and then homogenized together in 1 M acetic acid (AcOH) containing 20 mM HCl for 10 min in a whirling blender.…”

Section: Extraction and Purificationmentioning

confidence: 99%

Mitocryptide-2: Purification, Identification, and Characterization of a Novel Cryptide That Activates Neutrophils

Mukai

Seki²,

Nakano³

et al. 2009

The Journal of Immunology

View full text Add to dashboard Cite

Neutrophils are a class of leukocytes involved in innate immunity by monitoring and scavenging invading microorganisms and toxic substances. The actions of neutrophils in damaged tissues are still not well understood, particularly in the early stage of inflammation, and as-yet-unknown neutrophil-activating substances are proposed to induce their acute transmigration and activation. Here, we isolated and identified from porcine hearts a neutrophil-activating peptide. Structural analyses indicated that the primary structure of this peptide is formyl-Met-Thr-Asn-Ile-Arg-Lys-Ser-His-Pro-Leu-Met-Lys-Ile-Ile-Asn, which is identical to that of the N-terminal pentadecapeptide of porcine mitochondrial cytochrome b; we therefore named the newly isolated peptide “mitocryptide-2” (MCT-2), since we have recently purified and identified mitocryptide-1, a different class of a neutrophil-activating peptide. Synthetic MCT-2 and its human homolog hMCT-2 induced β-hexosaminidase release in and chemotaxis of HL-60 cells differentiated into neutrophilic/granulocytic cells. The induction of β-hexosaminidase release, chemotaxis, and the increase in the intracellular free Ca2+ concentration by hMCT-2 were completely suppressed by pertussis toxin, indicating the involvement of Gi- or Go-type G proteins in the signaling pathways. Moreover, MCT-2 and hMCT-2 also stimulated β-hexosaminidase secretion in human neutrophils isolated from peripheral blood in a concentration-dependent manner. Additionally, these peptides partially competed with [3H]formyl-Met-Leu-Phe binding to HL-60 cells differentiated into neutrophilic/granulocytic cells, presenting the possibility that the receptor for MCT-2 and hMCT-2 is one of the formyl peptide receptors. These results demonstrate that MCT-2 and its human homolog hMCT-2 are cryptides that activate neutrophils, thus suggesting the presence of regulatory mechanisms involving such mitocryptides in innate immunity.

show abstract

Section: Extraction and Purificationmentioning

confidence: 99%

Mitocryptide-2: Purification, Identification, and Characterization of a Novel Cryptide That Activates Neutrophils

Mukai

Seki²,

Nakano³

et al. 2009

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…More appropriate nomenclature for these peptides is the neurokinin family, since these are synthesized and stored in nervous structures and act as neurotransmitters. The neurokinins include the physalaemin-like compound, neurokinin A (NKA, also called neuromedin L or substance K) and neurokinin B (NKB, also called neuromedin K) (Kimura et al, 1983;Kangawa et al, 1983;Minamino et al, 1984). The occurrence of several neurokinins in mammalian tissues suggests the existence of different types of neurokinin receptors.…”

Section: Introductionmentioning

confidence: 99%

Specific neurokinin receptors mediate plasma extravasation in the rat knee joint

Lam

Ferrell

1991

British J Pharmacology

View full text Add to dashboard Cite

1 Plasma extravasation in the rat knee joint was induced by intra-articular injection of neurokinins and specific neurokinin receptor agonists.2 Pronounced plasma extravasation was produced by substance P (SP, 4-185,gM) 4 Injections of SP or NKA into the synovial cavity of the rat knee were equally effective in producing marked plasma extravasation in remote sites such as the forelimb and hindlimb paws. 5 Co-administration experiments showed that the effects of SP were synergistic with NKA or the NK1 receptor agonist, but not with CGRP or the NK2 receptor agonist. 6 The rank order of potency was NK1 agonist > SP > NKB > NK2 agonist suggesting that NK1 receptors mediate plasma extravasation in the rat knee joint.

show abstract

“…Recently, tachykinins other than SP, namely neurokinin A and B (NKA and NKB), have been demonstrated in mammalian tissues (Kimura et al, 1983;Kangawa et al, 1983;Minamino et al, 1984) and immunocytochemical studies have demonstrated the coexistence of SP-and NKA-like peptides in certain sensory nerve fibres (Dalsgaard et al, 1985;Hua et al, 1985;Sundler et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Substance P, neurokinin A and neurokinin B in the ocular response to injury in the rabbit

Beding-Barnekow¹,

Brodin²,

Håkanson³

1988

British J Pharmacology

View full text Add to dashboard Cite

1 Substance P (SP) and neurokinin A-(NKA)/neurokinin B (NKB)-like immunoreactivity (LI) were measured by radioimmunoassay in extracts of the rabbit uvea. The iris-ciliary body complex contained 3-4 times more NKA/NKB-LI than SP-LI. Tachykinins are thought to mediate many of the responses to ocular injury in the rabbit. Their possible role in the miosis and breakdown of the blood-aqueous barrier (BAB) was studied in vitro and in vivo. 2 In vitro, NKA had a more short-lasting contractile effect on the sphincter pupillae muscle than either SP or NKB, but SP was more potent than the other two. The tachykinin antagonist, spantide, dose-dependently suppressed the response to electrical stimulation (by 90% at 10-4M) and to the three tachykinins. An antiserum against SP (no cross-reaction with NKA or NKB) greatly suppressed the response to SP (by 90%) as well as to electrical field stimulation (by 40%). The responses to NKA and NKB were unaffected. 3 In vivo studies revealed that SP was more potent than NKA and NKB as a miotic. SP evoked a moderate breakdown of the BAB at high doses while NKA and NKB were virtually inactive. 4 We conclude that besides SP other tachykinins might play a role in the mediation of miosis in the rabbit eye but, of the three peptides investigated, only SP can be of importance for the breakdown of the BAB.

show abstract

Neuromedin K: A novel mammalian tachykinin identified in porcine spinal cord

Cited by 406 publications

References 9 publications

Mitocryptide-2: Purification, Identification, and Characterization of a Novel Cryptide That Activates Neutrophils

Mitocryptide-2: Purification, Identification, and Characterization of a Novel Cryptide That Activates Neutrophils

Specific neurokinin receptors mediate plasma extravasation in the rat knee joint

Substance P, neurokinin A and neurokinin B in the ocular response to injury in the rabbit

Contact Info

Product

Resources

About