Neurohypophyseal peptide inhibition of adenylate cyclase activity in fish gills The effect of environmental salinity

Guibbolini, Marielle; Lahlou, B.

doi:10.1016/0014-5793(87)80883-9

Cited by 30 publications

(15 citation statements)

References 21 publications

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“…The concentrations used in our work are within the range of the circulating peptide levels reported in fish (Henderson et al 1986, Perrott et al 1991, Warne et al 1994, Pierson et al 1995. As for the gills of SW-adapted rainbow trout, Guibbolini & Lahlou (1987) have shown that AVT and IT produced a maximal inhibition of membrane adenylate cyclase activity at 10 11 to 10 10 M. Sainsbury & Balment (1991) confirmed, on the same material, a similar maximal inhibitory effect of AVT at 10 10 to 10 9 M on cellular cAMP content. The present results obtained with sea bass gill cells showed a significant stimulation of I SC starting at 5 10 9 M of AVT and 5 10 10 M of IT, which compare approximately with the doses producing a maximal inhibitory effect in rainbow trout gill preparations.…”

Section: Avt and Itsupporting

confidence: 52%

“…In vitro studies showed that labelled AVT binds to specific receptors on eel gill cells (Guibbolini et al 1988). In rainbow trout gill plasma membranes, the AVT or IT binding was followed by a strong inhibition of the adenylate cyclase activity (in the absence of Ca 2+ ) mediated by a Gi protein sensitive to pertussis toxin and guanine nucleotides (Guibbolini & Lahlou 1987. The use of specific neurohypophysial analogues indicated that the effects of AVT and IT were mediated by a new type of receptor, functionally similar to the mammalian neurohypophysial V1a type (Guibbolini & Lahlou 1990).…”

Section: Introductionmentioning

confidence: 99%

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Neurohypophysial hormone regulation of Cl- secretion: physiological evidence for V1-type receptors in sea bass gill respiratory cells in culture

Guibbolini¹,

Avella²

2003

Journal of Endocrinology

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Neurohypophysial hormone receptors were studied in primary cultures of sea bass (Dicentrarchus labrax) gill respiratory-like cells grown on permeable supports. This preparation was previously shown to provide a functional model for investigating the hormonal regulation of Cl secretion.Under control conditions, the cultured monolayered epithelium had a short-circuit current (I SC ) of 3·5 1·1 µA cm 2 . This current had previously been identified as an active Cl secretion. The addition of increasing concentrations of the fish neurohypophysial hormones, arginine vasotocin (AVT) or isotocin (IT), elicited a concentration-dependent stimulation of the I SC . Maximal increases of 60·9 12·1% and 117·7 28·0% above the basal I SC value were obtained for 10 7 M AVT and IT respectively. Half-maximal effects were obtained for 3·1 10 9 M AVT and for 1·4 10 9 M IT. Mucosal application of 1·0 mM diphenylalamine-2-carboxylic acid (a specific blocker of Cl channels) after serosal addition of 5 10 8 M AVT or IT inhibited not only the basal but also the stimulated current, revealing a correlation with a hormone-dependent Cl transport.Specific V1 or V2 receptor analogues of vasopressin (mammalian hormone) were used to characterize the type of neurohypophysial hormone receptors pharmaco- ]-vasopressin used at the same concentration had no significant effect. In contrast, similar experiments carried out in the presence of 10 8 M IT showed that both antagonists significantly reduced the IT-stimulated Cl secretion, with the efficiency of the V1 receptor antagonist being significantly greater than that of the V2.This study provides evidence for neurohypophysial hormone control of Cl secretion in fish cultured gill respiratory cells. It suggests that on a physiological basis the hormonal effect is shared by the two peptides present in fish neurohypophysis (AVT and IT), acting by means of two distinct, although pharmacologically similar, V1-type receptors (according to the mammalian classification). These specific receptors are expected to play an important role in controlling ion homeostasis in seawater fish.

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Section: Avt and Itsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Neurohypophysial hormone regulation of Cl- secretion: physiological evidence for V1-type receptors in sea bass gill respiratory cells in culture

Guibbolini¹,

Avella²

2003

Journal of Endocrinology

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“…In our study, not only did the neurohypophysial peptides strongly inhibit the stimulatory effect, but the basal activity ( Fig. 1) was also reduced by AVT (50% inhibition), a fact only observed in a few cases: 15-25% inhibition in human platelets (Vanderwel et al 1983), 40% in trout gill plasma membranes (Guibbolini & Lahlou 1987). Secondly, in our model, not only did AVT and IT decrease cAMP production but they also behaved (n=6 for the V 1 antagonist and n=5 for the V 2 antagonist) and are expressed as percentages of maximal effect.…”

Section: Discussionsupporting

confidence: 49%

“…also exist in fish, namely in eel liver (Moon & Mommsen 1990) and in trout kidney (Perrott et al 1993), although the concentrations of hormone used in their experiments were high (D max between 10 -6 -10 -5 M). Our previous work on peripheral vasotocin receptors in lower vertebrates emphasized the 'V 1 ' feature of these receptors in such distinct organs as trout pituitary and gill (Guibbolini & Lahlou 1987, Guibbolini & Lahlou 1990, Pierson et al 1996. Recently, in the teleost fish Catostomus commersoni, the molecular structure of the vasotocin receptor was established by Mahlmann et al (1994) and that of the isotocin receptor by Hausmann et al (1995).…”

Section: Discussionmentioning

confidence: 99%

“…The final volume was 200 µl containing 100 µl equilibrated cell preparation (on average: 3·5 10 6 cells or 0·6 mg protein/100 µl cell preparation) in HMEM and 20 µl hormone(s). Because inhibitions are often larger or better revealed when the target system is stimulated by another hormone (Guibbolini & Lahlou 1987, 5 10 -8 M glucagon were added to the incubation medium. When hormones and analogues were considered together in the assay, they were added simultaneously in the incubation medium.…”

Section: Incubation Procedures and Camp Determinationmentioning

confidence: 99%

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Neurohypophysial hormone receptors and second messengers in trout hepatocytes

Guibbolini

Pierson²,

Lahlou³

2000

Journal of Endocrinology

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Neurohypophysial hormone receptors and second messengers were studied in trout (Oncorhynchus mykiss) hepatocytes. Arginine vasotocin (AVT) and isotocin (IT) elicited a concentration-dependent inhibition of cAMP accumulation in the presence of 5 10 -8 M glucagon (maximal effect for 4·5 10 -7 M and 1·4 10 -7 M, half-maximal effect for 2·1 10 -8 M and 0·7 10 -8 M, AVT and IT respectively). The effect of glucagon was inhibited up to 90% by AVT and 80% by IT. While AVT inhibited (up to 50%) the basal cAMP production, IT had no such action.Specific V 1 or V 2 analogues (with reference to vasopressin in mammals) were used for pharmacological characterization of the type of neurohypophysial hormone receptor involved in this inhibition. The ]-vasopressin had no such effect. In this particular case, maximal and half-maximal effects of the V 1 antagonist were obtained for 2·3 10 -6 M and 1·2 10 -6 M respectively.Changes in intracellular calcium content were measured using the fluorescent probe FURA-2/AM. AVT and IT elicited a concentration-dependent increase in Ca 2+ accumulation. The comparison of the effect of 10 -8 M agonists versus AVT showed the following order of potency: AVT=IT>V 1 agonist>V 2 agonist. The V 1 antagonist reversed the AVT-induced Ca 2+ accumulation whereas the V 2 antagonist had no such effect.These results are taken as evidence for the presence in trout hepatocytes of neurohypophysial hormone receptors functionally close to the V 1a -type linked to cAMP production and Ca 2+ mobilization.

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Cell signaling and ion transport across the fish gill epithelium

Evans

2002

J. Exp. Zool.

142

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A large array of circulating and local signaling agents modulate transport of ions across the gill epithelium of fishes by either affecting transport directly or by altering the size and distribution of transporting cells in the epithelium. In some cases, these transport effects are in addition to cardiovascular effects of the same agents, which may affect the perfusion pathways in the gill vasculature and, in turn, affect epithelial transport indirectly. Prolactin is generally considered to function in freshwater, because it is the only agent that allows survival of some hypophysectomized fish species in freshwater. It appears to function by either reducing branchial permeability, Na,K-activated ATPase activity, or reducing the density of chloride cells. Cortisol was initially considered to produce virtually opposite effects (e.g., stimulation of Na,K-activated ATPase and of chloride cell size and density), but more recent studies have found that this steroid stimulates ionic uptake in freshwater fishes, as well as the activity of H-ATPase, an enzyme thought to be central to ionic uptake. Thus, cortisol may function in both high and low salinities. Growth hormone and insulin-like growth factor appear to act synergistically to affect ion regulation in seawater fishes, stimulating both Na,K-activated ATPase and Na-K-2Cl co-transporter activity, and chloride cell size, independent of their effects on growth. Some of the effects of the GH-IGF axis may be via stimulation of the number of cortisol receptors. Thyroid hormones appear to affect seawater ion regulation indirectly, by stimulating the GH-IGF axis. Natriuretic peptides were initially thought to stimulate gill ionic extrusion, but recent studies have not corroborated this finding, so it appears that the major mode of action of these peptides may be reduction of salt loading by inhibition of oral ingestion and intestinal ionic uptake. Receptors for both arginine vasotocin and angiotensin have been described in the gill epithelium, but their respective roles and importance in fish ion regulation remains unknown. The gill epithelium may be affected by both circulating and local adrenergic agents, and a variety of studies have demonstrated that stimulation of alpha-adrenergic versus beta-adrenergic receptors produces inhibition or stimulation of active salt extrusion, respectively. Local effectors, such as prostaglandins, nitric oxide, and endothelin, may affect active salt extrusion as well as gill perfusion. Recent studies have suggested that the endothelin inhibition of salt extrusion is actually mediated by the release of both NO and prostaglandins. It is hoped that modern molecular techniques, combined with physiological measurements, will allow the dissection of the relative roles in ion transport across the fish gill epithelium of this surprisingly large array of putative signaling agents.

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Neurohypophyseal peptide inhibition of adenylate cyclase activity in fish gills The effect of environmental salinity

Cited by 30 publications

References 21 publications

Neurohypophysial hormone regulation of Cl- secretion: physiological evidence for V1-type receptors in sea bass gill respiratory cells in culture

Neurohypophysial hormone regulation of Cl- secretion: physiological evidence for V1-type receptors in sea bass gill respiratory cells in culture

Neurohypophysial hormone receptors and second messengers in trout hepatocytes

Cell signaling and ion transport across the fish gill epithelium

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