Neuroglobin and cytoglobin overexpression protects human SH-SY5Y neuroblastoma cells against oxidative stress-induced cell death

Fordel, Elke; Thijs, Liesbet; Martinet, Wim; Lenjou, Marc; Laufs, Tilmann; Bockstaele, Dirk Van; Moëns, Luc; Dewilde, Sylvia

doi:10.1016/j.neulet.2006.09.027

Cited by 148 publications

(140 citation statements)

References 19 publications

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“…Knockdown of Ngb rendered these cultures more vulnerable to hypoxia, whereas forced overexpression of Ngb in a cell line derived from hippocampal neurons conferred relative protection from hypoxia. In another study, cultured human neuroblastoma cells transfected with a Ngb-(or Cgb-) expressing plasmid were resistant to oxidative injury induced by H 2 O 2 (Fordel et al, 2006). In vivo studies yielded similar results.…”

Section: Introductionmentioning

confidence: 78%

A neuroglobin-overexpressing transgenic mouse

Khan¹,

Sun²,

Jin³

et al. 2007

Gene

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Neuroglobin (Ngb) is a recently discovered vertebrate globin expressed primarily in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons from these insults. However, its normal physiological role and the mechanism underlying its neuroprotective action are uncertain. We report production of a transgenic mouse in which Ngb is overexpressed under the control of the chicken β-actin promoter. This mouse should prove helpful for studying Ngb-mediated effects in vitro and in vivo.

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Section: Introductionmentioning

confidence: 78%

A neuroglobin-overexpressing transgenic mouse

Khan¹,

Sun²,

Jin³

et al. 2007

Gene

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“…Recent studies of neuronal cancer cells support the finding of the antioxidative stress effects of Cygb. 10,11 In addition to Cygb, recent studies reported that hemoglobin 28 and myoglobin 45 also scavenge radicals, which emphasizes a role for globin proteins as antioxidants. Collectively, these in vitro studies suggest a novel antioxidative role for globins in addition to their known conventional facilitation of oxygen transport, and support the finding that Cygb serves as a defensive mechanism against oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Cytoglobin, a Novel Member of the Globin Family, Protects Kidney Fibroblasts against Oxidative Stress under Ischemic Conditions

Nishi

Inagi

Kawada

et al. 2011

The American Journal of Pathology

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“…In addition, Ngb-overexpressing transgenic mice are resistant to cerebral infarction from occlusion of the middle cerebral artery [10,11]. Ngb also reduces the toxicity of H 2 O 2 [5], paraquat [5] and β-amyloid [13] in vitro. However, the mechanisms that underlie neuroprotection by Ngb are unknown.…”

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confidence: 99%

Neuroglobin protects against nitric oxide toxicity

Jin

Mao

Xie

et al. 2008

Neuroscience Letters

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Neuroglobin (Ngb) is a novel vertebrate globin expressed principally in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons against these insults. The mechanism of Ngb's protective action is unknown, but its ability to bind NO suggests that NO scavenging might be involved. To test this hypothesis, we treated wild type and Ngb-transfected HN33 (mouse hippocampal neuron × N18TG2 neuroblastoma) cells with NO donors and compared their sensitivity to NO-induced cell death. Ngb overexpression shifted concentration-toxicity curves to the right, indicating reduced susceptibility to NO or is metabolites. The results suggest that the ability of Ngb to neutralize the neurotoxic effects of reactive nitrogen species may be an important contributor to its neuroprotective properties. KeywordsNeuroglobin; nitric oxide; hypoxia Neuroglobin (Ngb) is a monomeric globin that is distantly related to hemoglobin and myoglobin and is expressed predominantly in brain neurons [3]. Ngb expression is induced by neuronal hypoxia and cerebral ischemia [21] and Ngb, in turn, appears to modulate hypoxicischemic brain injury. Thus, enhancing Ngb expression reduces-and knocking down Ngb expression increases-hypoxic neuronal injury in vitro [21] and ischemic cerebral injury in vivo [22]. In addition, Ngb-overexpressing transgenic mice are resistant to cerebral infarction from occlusion of the middle cerebral artery [10,11]. Ngb also reduces the toxicity of H 2 O 2 [5], paraquat [5] and β-amyloid [13] in vitro. However, the mechanisms that underlie neuroprotection by Ngb are unknown.Ancestral globins served primarily as NO oxygenases, converting NO to nitrate, whereas their O 2 -transporting function evolved later [20]. This primordial oxygenase activity is still apparent in the capacity of hemoglobin [6] and myoglobin [4] to scavenge NO, and appears to have pathophysiological significance, since overexpression of inducible NO synthase (NOS) causes heart failure in myoglobin-knockout mice [7]. Because Ngb binds NO [23], its neuroprotective action may also relate to NO scavenging [1,8].Several lines of evidence point to a connection between Ngb and NO. In addition to the ability of Ngb to bind NO [23], some studies have shown parallelism between the distribution of Ngb and of neuronal NOS (nNOS) [15], and NOS expression is increased in several tissues of Ngboverexpressing transgenic mice [11]. Autooxidation of Ngb yields NgbO 2 , which is thought to react rapidly with NO to form a peroxynitrite (ONOO − )-bound intermediate that decays to * To whom correspondence should be addressed: dgreenberg@buckinstitute.org Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered ...

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Neuroglobin and cytoglobin overexpression protects human SH-SY5Y neuroblastoma cells against oxidative stress-induced cell death

Cited by 148 publications

References 19 publications

A neuroglobin-overexpressing transgenic mouse

A neuroglobin-overexpressing transgenic mouse

Cytoglobin, a Novel Member of the Globin Family, Protects Kidney Fibroblasts against Oxidative Stress under Ischemic Conditions

Neuroglobin protects against nitric oxide toxicity

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